Application of d-SPE before SPE and HPLC-FLD to Analyze Bisphenols in Human Breast Milk Samples
In this study, we propose a simple, cost-effective, and sensitive high-performance liquid chromatography with fluorescence detection (HPLC-FLD) for the simultaneous determination of seven bisphenols (bisphenol F (BPF), bisphenol E (BPE), bisphenol B (BPB), BADGE (bisphenol A diglycidyl ether), BADGE...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-08-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/26/16/4930 |
| _version_ | 1797522698429005824 |
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| author | Tomasz Tuzimski Szymon Szubartowski |
| author_facet | Tomasz Tuzimski Szymon Szubartowski |
| author_sort | Tomasz Tuzimski |
| collection | DOAJ |
| description | In this study, we propose a simple, cost-effective, and sensitive high-performance liquid chromatography with fluorescence detection (HPLC-FLD) for the simultaneous determination of seven bisphenols (bisphenol F (BPF), bisphenol E (BPE), bisphenol B (BPB), BADGE (bisphenol A diglycidyl ether), BADGE∙2H<sub>2</sub>O, BADGE∙H<sub>2</sub>O, BADGE∙2HCl) in human breast milk samples. The dispersive solid phase extraction (d-SPE) coupled with solid phase extraction (SPE) procedure performed well for the majority of the analytes with recoveries in the range 57–88% and relative standard deviations (RSD%) of less than 9.4%. During the d-SPE stage, no significant matrix effect was observed thanks to the application of different pairs of salts such as zirconium-dioxide-based sorbents (Z-Sep or Z-Sep +) and primary secondary amine (PSA) or QuEChERS Enhanced Matrix Removal-Lipid (EMR-Lipid) and PSA. The method limits of quantification (mLOQs) for all investigated analytes were set at satisfactory low values in the range 171.89–235.11 ng mL<sup>−1</sup>. Analyte concentrations were determined as the average value from human breast milk matrix samples. The results show that the d-SPE/SPE procedure, especially with the application of EMR-Lipid and PSA, could be used for further bisphenol analyses in human breast milk samples. |
| first_indexed | 2024-03-10T08:33:07Z |
| format | Article |
| id | doaj.art-5265e150d97b4af5ba28762671d082ef |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T08:33:07Z |
| publishDate | 2021-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-5265e150d97b4af5ba28762671d082ef2023-11-22T08:54:05ZengMDPI AGMolecules1420-30492021-08-012616493010.3390/molecules26164930Application of d-SPE before SPE and HPLC-FLD to Analyze Bisphenols in Human Breast Milk SamplesTomasz Tuzimski0Szymon Szubartowski1Department of Physical Chemistry, Medical University of Lublin, Chodźki 4a, 20-093 Lublin, PolandDepartment of Physical Chemistry, Medical University of Lublin, Chodźki 4a, 20-093 Lublin, PolandIn this study, we propose a simple, cost-effective, and sensitive high-performance liquid chromatography with fluorescence detection (HPLC-FLD) for the simultaneous determination of seven bisphenols (bisphenol F (BPF), bisphenol E (BPE), bisphenol B (BPB), BADGE (bisphenol A diglycidyl ether), BADGE∙2H<sub>2</sub>O, BADGE∙H<sub>2</sub>O, BADGE∙2HCl) in human breast milk samples. The dispersive solid phase extraction (d-SPE) coupled with solid phase extraction (SPE) procedure performed well for the majority of the analytes with recoveries in the range 57–88% and relative standard deviations (RSD%) of less than 9.4%. During the d-SPE stage, no significant matrix effect was observed thanks to the application of different pairs of salts such as zirconium-dioxide-based sorbents (Z-Sep or Z-Sep +) and primary secondary amine (PSA) or QuEChERS Enhanced Matrix Removal-Lipid (EMR-Lipid) and PSA. The method limits of quantification (mLOQs) for all investigated analytes were set at satisfactory low values in the range 171.89–235.11 ng mL<sup>−1</sup>. Analyte concentrations were determined as the average value from human breast milk matrix samples. The results show that the d-SPE/SPE procedure, especially with the application of EMR-Lipid and PSA, could be used for further bisphenol analyses in human breast milk samples.https://www.mdpi.com/1420-3049/26/16/4930bisphenolshuman breast milk sampleshigh-performance liquid chromatography (HPLC)fluorescence detector (FLD)dispersive solid phase extraction (d-SPE)/solid phase extraction (SPE)BADGE (bisphenol A diglycidyl ether) and its derivatives |
| spellingShingle | Tomasz Tuzimski Szymon Szubartowski Application of d-SPE before SPE and HPLC-FLD to Analyze Bisphenols in Human Breast Milk Samples Molecules bisphenols human breast milk samples high-performance liquid chromatography (HPLC) fluorescence detector (FLD) dispersive solid phase extraction (d-SPE)/solid phase extraction (SPE) BADGE (bisphenol A diglycidyl ether) and its derivatives |
| title | Application of d-SPE before SPE and HPLC-FLD to Analyze Bisphenols in Human Breast Milk Samples |
| title_full | Application of d-SPE before SPE and HPLC-FLD to Analyze Bisphenols in Human Breast Milk Samples |
| title_fullStr | Application of d-SPE before SPE and HPLC-FLD to Analyze Bisphenols in Human Breast Milk Samples |
| title_full_unstemmed | Application of d-SPE before SPE and HPLC-FLD to Analyze Bisphenols in Human Breast Milk Samples |
| title_short | Application of d-SPE before SPE and HPLC-FLD to Analyze Bisphenols in Human Breast Milk Samples |
| title_sort | application of d spe before spe and hplc fld to analyze bisphenols in human breast milk samples |
| topic | bisphenols human breast milk samples high-performance liquid chromatography (HPLC) fluorescence detector (FLD) dispersive solid phase extraction (d-SPE)/solid phase extraction (SPE) BADGE (bisphenol A diglycidyl ether) and its derivatives |
| url | https://www.mdpi.com/1420-3049/26/16/4930 |
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