Antifertility properties of Centella asiatica ethanolic extract as a contraceptive agent: Preliminary study of sperm proteomic
Objective: To investigate the antifertility properties of Centella asiatica L. ethanolic extract and identify the sperm proteomic changes in treated male rats.Methods: A total of 32 male Sprague-Dawley rats were divided into two groups: control group (n=16) received distilled water and treatment gro...
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2017-01-01
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| Series: | Asian Pacific Journal of Reproduction |
| Subjects: | |
| Online Access: | http://www.apjr.net/article.asp?issn=2305-0500;year=2017;volume=6;issue=5;spage=212;epage=216;aulast=Yunianto |
| _version_ | 1828472865475788800 |
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| author | Irfan Yunianto Nurul Ain Kamar Bashah Mahanem Mat Noor |
| author_facet | Irfan Yunianto Nurul Ain Kamar Bashah Mahanem Mat Noor |
| author_sort | Irfan Yunianto |
| collection | DOAJ |
| description | Objective: To investigate the antifertility properties of Centella asiatica L. ethanolic extract and identify the sperm proteomic changes in treated male rats.Methods: A total of 32 male Sprague-Dawley rats were divided into two groups: control group (n=16) received distilled water and treatment group (n=16) which received 300 mg/kg of Centella asiatica L. extract for 42 d. After the treatment period, the number of implantation sites was recorded and the sperm proteomic changes were analysed by 2D gel electrophoresis. In addition, the expression of protein spots was quantified by MALDI-TOF analysis.Results: Centella asiatica L. extract resulted in low number of implantation sites in the treatment group (100.00±2.82) compared to the control group (183.00±2.14). The percentage of infertile male rats in the treatment group was higher (43.75%) compared to the control group (18.75%). Proteomic analysis showed the expression of protein spots identified in the treatment group decreased with 234 spots compared to the control group with 282 spots.Conclusions: The results revealed that there was antifertility activity in the male rats with the administration of Centella asiatica L. ethanol extract and the identified proteins could provide understanding on the adverse effect of Centella asiatica in male reproductive system. |
| first_indexed | 2024-12-11T05:36:06Z |
| format | Article |
| id | doaj.art-526a3add86744ad19d65d39a3822c95d |
| institution | Directory Open Access Journal |
| issn | 2305-0500 2305-0519 |
| language | English |
| last_indexed | 2024-12-11T05:36:06Z |
| publishDate | 2017-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Asian Pacific Journal of Reproduction |
| spelling | doaj.art-526a3add86744ad19d65d39a3822c95d2022-12-22T01:19:17ZengWolters Kluwer Medknow PublicationsAsian Pacific Journal of Reproduction2305-05002305-05192017-01-016521221610.4103/2305-0500.215931Antifertility properties of Centella asiatica ethanolic extract as a contraceptive agent: Preliminary study of sperm proteomicIrfan YuniantoNurul Ain Kamar BashahMahanem Mat NoorObjective: To investigate the antifertility properties of Centella asiatica L. ethanolic extract and identify the sperm proteomic changes in treated male rats.Methods: A total of 32 male Sprague-Dawley rats were divided into two groups: control group (n=16) received distilled water and treatment group (n=16) which received 300 mg/kg of Centella asiatica L. extract for 42 d. After the treatment period, the number of implantation sites was recorded and the sperm proteomic changes were analysed by 2D gel electrophoresis. In addition, the expression of protein spots was quantified by MALDI-TOF analysis.Results: Centella asiatica L. extract resulted in low number of implantation sites in the treatment group (100.00±2.82) compared to the control group (183.00±2.14). The percentage of infertile male rats in the treatment group was higher (43.75%) compared to the control group (18.75%). Proteomic analysis showed the expression of protein spots identified in the treatment group decreased with 234 spots compared to the control group with 282 spots.Conclusions: The results revealed that there was antifertility activity in the male rats with the administration of Centella asiatica L. ethanol extract and the identified proteins could provide understanding on the adverse effect of Centella asiatica in male reproductive system.http://www.apjr.net/article.asp?issn=2305-0500;year=2017;volume=6;issue=5;spage=212;epage=216;aulast=YuniantoCentella asiatica LAntifertilityMALDI-TOFSperm proteomic |
| spellingShingle | Irfan Yunianto Nurul Ain Kamar Bashah Mahanem Mat Noor Antifertility properties of Centella asiatica ethanolic extract as a contraceptive agent: Preliminary study of sperm proteomic Asian Pacific Journal of Reproduction Centella asiatica L Antifertility MALDI-TOF Sperm proteomic |
| title | Antifertility properties of Centella asiatica ethanolic extract as a contraceptive agent: Preliminary study of sperm proteomic |
| title_full | Antifertility properties of Centella asiatica ethanolic extract as a contraceptive agent: Preliminary study of sperm proteomic |
| title_fullStr | Antifertility properties of Centella asiatica ethanolic extract as a contraceptive agent: Preliminary study of sperm proteomic |
| title_full_unstemmed | Antifertility properties of Centella asiatica ethanolic extract as a contraceptive agent: Preliminary study of sperm proteomic |
| title_short | Antifertility properties of Centella asiatica ethanolic extract as a contraceptive agent: Preliminary study of sperm proteomic |
| title_sort | antifertility properties of centella asiatica ethanolic extract as a contraceptive agent preliminary study of sperm proteomic |
| topic | Centella asiatica L Antifertility MALDI-TOF Sperm proteomic |
| url | http://www.apjr.net/article.asp?issn=2305-0500;year=2017;volume=6;issue=5;spage=212;epage=216;aulast=Yunianto |
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