High IFN-γ Release and Impaired Capacity of Multi-Cytokine Secretion in IGRA Supernatants Are Associated with Active Tuberculosis.
Interferon gamma (IFN-γ) release assays (IGRAs) detect Mycobacterium tuberculosis (Mtb) infection regardless of the active (ATB) or latent (LTBI) forms of tuberculosis (TB). In this study, Mtb-specific T cell response against region of deletion 1 (RD1) antigens were explored by a microbead multiplex...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2016-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC5014470?pdf=render |
| _version_ | 1828398385047011328 |
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| author | Séverine Carrère-Kremer Pierre-Alain Rubbo Amandine Pisoni Sophie Bendriss Grégory Marin Marianne Peries Karine Bolloré Dominique Terru Sylvain Godreuil Arnaud Bourdin Philippe Van de Perre Edouard Tuaillon |
| author_facet | Séverine Carrère-Kremer Pierre-Alain Rubbo Amandine Pisoni Sophie Bendriss Grégory Marin Marianne Peries Karine Bolloré Dominique Terru Sylvain Godreuil Arnaud Bourdin Philippe Van de Perre Edouard Tuaillon |
| author_sort | Séverine Carrère-Kremer |
| collection | DOAJ |
| description | Interferon gamma (IFN-γ) release assays (IGRAs) detect Mycobacterium tuberculosis (Mtb) infection regardless of the active (ATB) or latent (LTBI) forms of tuberculosis (TB). In this study, Mtb-specific T cell response against region of deletion 1 (RD1) antigens were explored by a microbead multiplex assay performed in T-SPOT TB assay (T-SPOT) supernatants from 35 patients with ATB and 115 patients with LTBI. T-SPOT is positive when over 7 IFN-γ secreting cells (SC)/250 000 peripheral blood mononuclear cells (PBMC) are enumerated. However, over 100 IFN-γ SC /250 000 PBMC were more frequently observed in the ATB group compared to the LTBI group. By contrast, lower cytokine concentrations and lower cytokine productions relative to IFN-γ secretion were observed for IL 4, IL-12, TNF-α, GM-CSF, Eotaxin and IFN-α when compared to LTBI. Thus, high IFN-γ release and low cytokine secretions in relation with IFN-γ production appeared as signatures of ATB, corroborating that multicytokine Mtb-specific response against RD1 antigens reflects host capacity to contain TB reactivation. In this way, testing cytokine profile in IGRA supernatants would be helpful to improve ATB screening strategy including immunologic tests. |
| first_indexed | 2024-12-10T09:00:39Z |
| format | Article |
| id | doaj.art-526cc992613f48c8b5f13aaa6e8d248b |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-10T09:00:39Z |
| publishDate | 2016-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-526cc992613f48c8b5f13aaa6e8d248b2022-12-22T01:55:18ZengPublic Library of Science (PLoS)PLoS ONE1932-62032016-01-01119e016213710.1371/journal.pone.0162137High IFN-γ Release and Impaired Capacity of Multi-Cytokine Secretion in IGRA Supernatants Are Associated with Active Tuberculosis.Séverine Carrère-KremerPierre-Alain RubboAmandine PisoniSophie BendrissGrégory MarinMarianne PeriesKarine BolloréDominique TerruSylvain GodreuilArnaud BourdinPhilippe Van de PerreEdouard TuaillonInterferon gamma (IFN-γ) release assays (IGRAs) detect Mycobacterium tuberculosis (Mtb) infection regardless of the active (ATB) or latent (LTBI) forms of tuberculosis (TB). In this study, Mtb-specific T cell response against region of deletion 1 (RD1) antigens were explored by a microbead multiplex assay performed in T-SPOT TB assay (T-SPOT) supernatants from 35 patients with ATB and 115 patients with LTBI. T-SPOT is positive when over 7 IFN-γ secreting cells (SC)/250 000 peripheral blood mononuclear cells (PBMC) are enumerated. However, over 100 IFN-γ SC /250 000 PBMC were more frequently observed in the ATB group compared to the LTBI group. By contrast, lower cytokine concentrations and lower cytokine productions relative to IFN-γ secretion were observed for IL 4, IL-12, TNF-α, GM-CSF, Eotaxin and IFN-α when compared to LTBI. Thus, high IFN-γ release and low cytokine secretions in relation with IFN-γ production appeared as signatures of ATB, corroborating that multicytokine Mtb-specific response against RD1 antigens reflects host capacity to contain TB reactivation. In this way, testing cytokine profile in IGRA supernatants would be helpful to improve ATB screening strategy including immunologic tests.http://europepmc.org/articles/PMC5014470?pdf=render |
| spellingShingle | Séverine Carrère-Kremer Pierre-Alain Rubbo Amandine Pisoni Sophie Bendriss Grégory Marin Marianne Peries Karine Bolloré Dominique Terru Sylvain Godreuil Arnaud Bourdin Philippe Van de Perre Edouard Tuaillon High IFN-γ Release and Impaired Capacity of Multi-Cytokine Secretion in IGRA Supernatants Are Associated with Active Tuberculosis. PLoS ONE |
| title | High IFN-γ Release and Impaired Capacity of Multi-Cytokine Secretion in IGRA Supernatants Are Associated with Active Tuberculosis. |
| title_full | High IFN-γ Release and Impaired Capacity of Multi-Cytokine Secretion in IGRA Supernatants Are Associated with Active Tuberculosis. |
| title_fullStr | High IFN-γ Release and Impaired Capacity of Multi-Cytokine Secretion in IGRA Supernatants Are Associated with Active Tuberculosis. |
| title_full_unstemmed | High IFN-γ Release and Impaired Capacity of Multi-Cytokine Secretion in IGRA Supernatants Are Associated with Active Tuberculosis. |
| title_short | High IFN-γ Release and Impaired Capacity of Multi-Cytokine Secretion in IGRA Supernatants Are Associated with Active Tuberculosis. |
| title_sort | high ifn γ release and impaired capacity of multi cytokine secretion in igra supernatants are associated with active tuberculosis |
| url | http://europepmc.org/articles/PMC5014470?pdf=render |
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