Detection of Biofilm Production and Antibiotic Resistance Pattern in Clinical Isolates from Orthopaedic Implants Associated Infections
Introduction: Orthopaedic Implant associated infection is the major problem which leads to implant failure and economic burden to the patient. Implant-associated infections are due to the formation of biofilm at the implantation site leading to multidrug resistant organism. Aim: This study...
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| Format: | Article |
| Language: | English |
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ADICHUNCHANAGIRI INSTITUTE OF MEDICAL SCIENCES
2023-10-01
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| Series: | Journal of Medical Sciences and Health |
| Online Access: | https://jmsh.ac.in/articles/detection-of-biofilm-production-and-antibiotic-resistance-pattern-in-clinical-isolates-from-orthopaedic-implants-associated-infections |
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| author | R Shakthi D Venkatesha |
| author_facet | R Shakthi D Venkatesha |
| author_sort | R Shakthi |
| collection | DOAJ |
| description | Introduction: Orthopaedic Implant associated infection is the major problem which leads to implant failure and economic burden to the patient. Implant-associated infections are due to the formation of biofilm at the implantation site leading to multidrug resistant organism. Aim: This study was done to evaluate the causative organisms, their antibiotic sensitivity pattern and their ability to form biofilm over the Implants used in Orthopaedic surgeries. Material and methods: This study was carried out in the department of Microbiology, Adichunchanagiri institute of Medical sciences from September 2019 to August 2022. Swabs from 100 patients who had undergone orthopaedic implant or prosthetic surgery and presented with signs and symptoms of infection were collected and processed as per standard procedures. All the isolates were subjected to detect biofilm production by Congo red agar method, MRSA detection by Cefoxitin disc diffusion test, vancomycin resistant Enterococcus by using Vancomycin (Minimum inhibitory concentration) MIC strips and Extended spectrum β lactamases (ESBL) production by phenotypic confirmatory combined-disc test. Results: Out of the 100 samples processed, culture positivity was observed in 72 specimens. Among them, Staphylococcus aureus 19(26%) was the predominant isolate and 44 (61%) are biofilm producers. 17 (89%) were MRSA (Methicillin resistance Staphylococcus aureus) strains and 15(37%) were ESBL producing strains. Conclusion: The appropriate pre- and post- operative care should be taken to prevent orthopaedic implant associated infections. Staphylococcus spp was the commonest isolate and its ability to produce biofilm indicates the need for an appropriate antibiotic policy and screening for MRSA carriers to reduce the infection. Keywords: Orthopaedic implant, Infection, Biofilm, MRSA, ESBL |
| first_indexed | 2024-03-11T14:34:13Z |
| format | Article |
| id | doaj.art-526e43b4b267446e9636af946795f054 |
| institution | Directory Open Access Journal |
| issn | 2394-9481 2394-949X |
| language | English |
| last_indexed | 2024-03-11T14:34:13Z |
| publishDate | 2023-10-01 |
| publisher | ADICHUNCHANAGIRI INSTITUTE OF MEDICAL SCIENCES |
| record_format | Article |
| series | Journal of Medical Sciences and Health |
| spelling | doaj.art-526e43b4b267446e9636af946795f0542023-10-31T05:20:41ZengADICHUNCHANAGIRI INSTITUTE OF MEDICAL SCIENCESJournal of Medical Sciences and Health2394-94812394-949X2023-10-019215716210.46347/jmsh.v9i2.23.5Detection of Biofilm Production and Antibiotic Resistance Pattern in Clinical Isolates from Orthopaedic Implants Associated InfectionsR ShakthiD Venkatesha Introduction: Orthopaedic Implant associated infection is the major problem which leads to implant failure and economic burden to the patient. Implant-associated infections are due to the formation of biofilm at the implantation site leading to multidrug resistant organism. Aim: This study was done to evaluate the causative organisms, their antibiotic sensitivity pattern and their ability to form biofilm over the Implants used in Orthopaedic surgeries. Material and methods: This study was carried out in the department of Microbiology, Adichunchanagiri institute of Medical sciences from September 2019 to August 2022. Swabs from 100 patients who had undergone orthopaedic implant or prosthetic surgery and presented with signs and symptoms of infection were collected and processed as per standard procedures. All the isolates were subjected to detect biofilm production by Congo red agar method, MRSA detection by Cefoxitin disc diffusion test, vancomycin resistant Enterococcus by using Vancomycin (Minimum inhibitory concentration) MIC strips and Extended spectrum β lactamases (ESBL) production by phenotypic confirmatory combined-disc test. Results: Out of the 100 samples processed, culture positivity was observed in 72 specimens. Among them, Staphylococcus aureus 19(26%) was the predominant isolate and 44 (61%) are biofilm producers. 17 (89%) were MRSA (Methicillin resistance Staphylococcus aureus) strains and 15(37%) were ESBL producing strains. Conclusion: The appropriate pre- and post- operative care should be taken to prevent orthopaedic implant associated infections. Staphylococcus spp was the commonest isolate and its ability to produce biofilm indicates the need for an appropriate antibiotic policy and screening for MRSA carriers to reduce the infection. Keywords: Orthopaedic implant, Infection, Biofilm, MRSA, ESBLhttps://jmsh.ac.in/articles/detection-of-biofilm-production-and-antibiotic-resistance-pattern-in-clinical-isolates-from-orthopaedic-implants-associated-infections |
| spellingShingle | R Shakthi D Venkatesha Detection of Biofilm Production and Antibiotic Resistance Pattern in Clinical Isolates from Orthopaedic Implants Associated Infections Journal of Medical Sciences and Health |
| title | Detection of Biofilm Production and Antibiotic Resistance Pattern in Clinical Isolates from Orthopaedic Implants Associated Infections |
| title_full | Detection of Biofilm Production and Antibiotic Resistance Pattern in Clinical Isolates from Orthopaedic Implants Associated Infections |
| title_fullStr | Detection of Biofilm Production and Antibiotic Resistance Pattern in Clinical Isolates from Orthopaedic Implants Associated Infections |
| title_full_unstemmed | Detection of Biofilm Production and Antibiotic Resistance Pattern in Clinical Isolates from Orthopaedic Implants Associated Infections |
| title_short | Detection of Biofilm Production and Antibiotic Resistance Pattern in Clinical Isolates from Orthopaedic Implants Associated Infections |
| title_sort | detection of biofilm production and antibiotic resistance pattern in clinical isolates from orthopaedic implants associated infections |
| url | https://jmsh.ac.in/articles/detection-of-biofilm-production-and-antibiotic-resistance-pattern-in-clinical-isolates-from-orthopaedic-implants-associated-infections |
| work_keys_str_mv | AT rshakthi detectionofbiofilmproductionandantibioticresistancepatterninclinicalisolatesfromorthopaedicimplantsassociatedinfections AT dvenkatesha detectionofbiofilmproductionandantibioticresistancepatterninclinicalisolatesfromorthopaedicimplantsassociatedinfections |