Surfactant uptake dynamics in mammalian cells elucidated with quantitative coherent anti-stokes Raman scattering microspectroscopy.
The mechanism of surfactant-induced cell lysis has been studied with quantitative coherent anti-Stokes Raman scattering (CARS) microspectroscopy. The dynamics of surfactant molecules as well as intracellular biomolecules in living Chinese Hamster Lung (CHL) cells has been examined for a low surfacta...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2014-01-01
|
| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3977816?pdf=render |
| _version_ | 1818543382816882688 |
|---|---|
| author | Masanari Okuno Hideaki Kano Kenkichi Fujii Kotatsu Bito Satoru Naito Philippe Leproux Vincent Couderc Hiro-o Hamaguchi |
| author_facet | Masanari Okuno Hideaki Kano Kenkichi Fujii Kotatsu Bito Satoru Naito Philippe Leproux Vincent Couderc Hiro-o Hamaguchi |
| author_sort | Masanari Okuno |
| collection | DOAJ |
| description | The mechanism of surfactant-induced cell lysis has been studied with quantitative coherent anti-Stokes Raman scattering (CARS) microspectroscopy. The dynamics of surfactant molecules as well as intracellular biomolecules in living Chinese Hamster Lung (CHL) cells has been examined for a low surfactant concentration (0.01 w%). By using an isotope labeled surfactant having CD bonds, surfactant uptake dynamics in living cells has been traced in detail. The simultaneous CARS imaging of the cell itself and the internalized surfactant has shown that the surfactant molecules is first accumulated inside a CHL cell followed by a sudden leak of cytosolic components such as proteins to the outside of the cell. This finding indicates that surfactant uptake occurs prior to the cell lysis, contrary to what has been believed: surface adsorption of surfactant molecules has been thought to occur first with subsequent disruption of cell membranes. Quantitative CARS microspectroscopy enables us to determine the molecular concentration of the surfactant molecules accumulated in a cell. We have also investigated the effect of a drug, nocodazole, on the surfactant uptake dynamics. As a result of the inhibition of tubulin polymerization by nocodazole, the surfactant uptake rate is significantly lowered. This fact suggests that intracellular membrane trafficking contributes to the surfactant uptake mechanism. |
| first_indexed | 2024-12-11T22:34:39Z |
| format | Article |
| id | doaj.art-526fa63e3fff4b899fda00af7163e301 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-11T22:34:39Z |
| publishDate | 2014-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-526fa63e3fff4b899fda00af7163e3012022-12-22T00:48:01ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-0194e9340110.1371/journal.pone.0093401Surfactant uptake dynamics in mammalian cells elucidated with quantitative coherent anti-stokes Raman scattering microspectroscopy.Masanari OkunoHideaki KanoKenkichi FujiiKotatsu BitoSatoru NaitoPhilippe LeprouxVincent CoudercHiro-o HamaguchiThe mechanism of surfactant-induced cell lysis has been studied with quantitative coherent anti-Stokes Raman scattering (CARS) microspectroscopy. The dynamics of surfactant molecules as well as intracellular biomolecules in living Chinese Hamster Lung (CHL) cells has been examined for a low surfactant concentration (0.01 w%). By using an isotope labeled surfactant having CD bonds, surfactant uptake dynamics in living cells has been traced in detail. The simultaneous CARS imaging of the cell itself and the internalized surfactant has shown that the surfactant molecules is first accumulated inside a CHL cell followed by a sudden leak of cytosolic components such as proteins to the outside of the cell. This finding indicates that surfactant uptake occurs prior to the cell lysis, contrary to what has been believed: surface adsorption of surfactant molecules has been thought to occur first with subsequent disruption of cell membranes. Quantitative CARS microspectroscopy enables us to determine the molecular concentration of the surfactant molecules accumulated in a cell. We have also investigated the effect of a drug, nocodazole, on the surfactant uptake dynamics. As a result of the inhibition of tubulin polymerization by nocodazole, the surfactant uptake rate is significantly lowered. This fact suggests that intracellular membrane trafficking contributes to the surfactant uptake mechanism.http://europepmc.org/articles/PMC3977816?pdf=render |
| spellingShingle | Masanari Okuno Hideaki Kano Kenkichi Fujii Kotatsu Bito Satoru Naito Philippe Leproux Vincent Couderc Hiro-o Hamaguchi Surfactant uptake dynamics in mammalian cells elucidated with quantitative coherent anti-stokes Raman scattering microspectroscopy. PLoS ONE |
| title | Surfactant uptake dynamics in mammalian cells elucidated with quantitative coherent anti-stokes Raman scattering microspectroscopy. |
| title_full | Surfactant uptake dynamics in mammalian cells elucidated with quantitative coherent anti-stokes Raman scattering microspectroscopy. |
| title_fullStr | Surfactant uptake dynamics in mammalian cells elucidated with quantitative coherent anti-stokes Raman scattering microspectroscopy. |
| title_full_unstemmed | Surfactant uptake dynamics in mammalian cells elucidated with quantitative coherent anti-stokes Raman scattering microspectroscopy. |
| title_short | Surfactant uptake dynamics in mammalian cells elucidated with quantitative coherent anti-stokes Raman scattering microspectroscopy. |
| title_sort | surfactant uptake dynamics in mammalian cells elucidated with quantitative coherent anti stokes raman scattering microspectroscopy |
| url | http://europepmc.org/articles/PMC3977816?pdf=render |
| work_keys_str_mv | AT masanariokuno surfactantuptakedynamicsinmammaliancellselucidatedwithquantitativecoherentantistokesramanscatteringmicrospectroscopy AT hideakikano surfactantuptakedynamicsinmammaliancellselucidatedwithquantitativecoherentantistokesramanscatteringmicrospectroscopy AT kenkichifujii surfactantuptakedynamicsinmammaliancellselucidatedwithquantitativecoherentantistokesramanscatteringmicrospectroscopy AT kotatsubito surfactantuptakedynamicsinmammaliancellselucidatedwithquantitativecoherentantistokesramanscatteringmicrospectroscopy AT satorunaito surfactantuptakedynamicsinmammaliancellselucidatedwithquantitativecoherentantistokesramanscatteringmicrospectroscopy AT philippeleproux surfactantuptakedynamicsinmammaliancellselucidatedwithquantitativecoherentantistokesramanscatteringmicrospectroscopy AT vincentcouderc surfactantuptakedynamicsinmammaliancellselucidatedwithquantitativecoherentantistokesramanscatteringmicrospectroscopy AT hiroohamaguchi surfactantuptakedynamicsinmammaliancellselucidatedwithquantitativecoherentantistokesramanscatteringmicrospectroscopy |