Metabolic network changes during skotomorphogenesis in Arabidopsis thaliana mutant (atdfb‐3)
Abstract The metabolic networks underlying skotomorphogenesis in seedlings remain relatively unknown. On the basis of our previous study on the folate metabolism in seedlings grown in darkness, the plastidial folylpolyglutamate synthetase gene (AtDFB) T‐DNA insertion Arabidopsis thaliana mutant (atd...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2022-11-01
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| Series: | Plant Direct |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/pld3.467 |
| _version_ | 1811215703549673472 |
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| author | Xingjuan Li Hongyan Meng Liqing Liu Cuiyun Hong Chunyi Zhang |
| author_facet | Xingjuan Li Hongyan Meng Liqing Liu Cuiyun Hong Chunyi Zhang |
| author_sort | Xingjuan Li |
| collection | DOAJ |
| description | Abstract The metabolic networks underlying skotomorphogenesis in seedlings remain relatively unknown. On the basis of our previous study on the folate metabolism in seedlings grown in darkness, the plastidial folylpolyglutamate synthetase gene (AtDFB) T‐DNA insertion Arabidopsis thaliana mutant (atdfb‐3) was examined. Under the nitrate‐sufficient condition, the mutant exhibited deficient folate metabolism and hypocotyl elongation, which affected skotomorphogenesis. Further analyses revealed changes to multiple intermediate metabolites related to carbon and nitrogen metabolism in the etiolated atdfb‐3 seedlings. Specifically, the sugar, polyol, and fatty acid contents decreased in the atdfb‐3 mutant under the nitrate‐sufficient condition, whereas the abundance of various organic acids and amino acids increased. In response to nitrate‐limited stress, multiple metabolites, including sugars, polyols, fatty acids, organic acids, and amino acids, accumulated more in the mutant than in the wild‐type control. The differences in the contents of multiple metabolites between the atdfb‐3 and wild‐type seedlings decreased following the addition of exogenous 5‐F‐THF under both nitrogen conditions. Additionally, the mutant accumulated high levels of one‐carbon metabolites, such as Cys, S‐adenosylmethionine, and S‐adenosylhomocysteine, under both nitrogen conditions. Thus, our data demonstrated that the perturbed folate metabolism in the atdfb‐3 seedlings, which was caused by the loss‐of‐function mutation to AtDFB, probably altered carbon and nitrogen metabolism, thereby modulating skotomorphogenesis. Furthermore, the study findings provide new evidence of the links among folate metabolism, metabolic networks, and skotomorphogenesis. |
| first_indexed | 2024-04-12T06:28:02Z |
| format | Article |
| id | doaj.art-52701a43b60f4d5998284781beb30864 |
| institution | Directory Open Access Journal |
| issn | 2475-4455 |
| language | English |
| last_indexed | 2024-04-12T06:28:02Z |
| publishDate | 2022-11-01 |
| publisher | Wiley |
| record_format | Article |
| series | Plant Direct |
| spelling | doaj.art-52701a43b60f4d5998284781beb308642022-12-22T03:44:06ZengWileyPlant Direct2475-44552022-11-01611n/an/a10.1002/pld3.467Metabolic network changes during skotomorphogenesis in Arabidopsis thaliana mutant (atdfb‐3)Xingjuan Li0Hongyan Meng1Liqing Liu2Cuiyun Hong3Chunyi Zhang4College of Bioengineering Beijing Polytechnic Beijing ChinaFujian Provincial Key Laboratory of Subtropical Plant Physiology and Biochemistry Fujian Institute of Subtropical Botany Xiamen ChinaFujian Provincial Key Laboratory of Subtropical Plant Physiology and Biochemistry Fujian Institute of Subtropical Botany Xiamen ChinaFujian Provincial Key Laboratory of Subtropical Plant Physiology and Biochemistry Fujian Institute of Subtropical Botany Xiamen ChinaBiotechnology Research Institute Chinese Academy of Agricultural Sciences Beijing ChinaAbstract The metabolic networks underlying skotomorphogenesis in seedlings remain relatively unknown. On the basis of our previous study on the folate metabolism in seedlings grown in darkness, the plastidial folylpolyglutamate synthetase gene (AtDFB) T‐DNA insertion Arabidopsis thaliana mutant (atdfb‐3) was examined. Under the nitrate‐sufficient condition, the mutant exhibited deficient folate metabolism and hypocotyl elongation, which affected skotomorphogenesis. Further analyses revealed changes to multiple intermediate metabolites related to carbon and nitrogen metabolism in the etiolated atdfb‐3 seedlings. Specifically, the sugar, polyol, and fatty acid contents decreased in the atdfb‐3 mutant under the nitrate‐sufficient condition, whereas the abundance of various organic acids and amino acids increased. In response to nitrate‐limited stress, multiple metabolites, including sugars, polyols, fatty acids, organic acids, and amino acids, accumulated more in the mutant than in the wild‐type control. The differences in the contents of multiple metabolites between the atdfb‐3 and wild‐type seedlings decreased following the addition of exogenous 5‐F‐THF under both nitrogen conditions. Additionally, the mutant accumulated high levels of one‐carbon metabolites, such as Cys, S‐adenosylmethionine, and S‐adenosylhomocysteine, under both nitrogen conditions. Thus, our data demonstrated that the perturbed folate metabolism in the atdfb‐3 seedlings, which was caused by the loss‐of‐function mutation to AtDFB, probably altered carbon and nitrogen metabolism, thereby modulating skotomorphogenesis. Furthermore, the study findings provide new evidence of the links among folate metabolism, metabolic networks, and skotomorphogenesis.https://doi.org/10.1002/pld3.467Arabidopsisfolatemetabolic networksskotomorphogenesis |
| spellingShingle | Xingjuan Li Hongyan Meng Liqing Liu Cuiyun Hong Chunyi Zhang Metabolic network changes during skotomorphogenesis in Arabidopsis thaliana mutant (atdfb‐3) Plant Direct Arabidopsis folate metabolic networks skotomorphogenesis |
| title | Metabolic network changes during skotomorphogenesis in Arabidopsis thaliana mutant (atdfb‐3) |
| title_full | Metabolic network changes during skotomorphogenesis in Arabidopsis thaliana mutant (atdfb‐3) |
| title_fullStr | Metabolic network changes during skotomorphogenesis in Arabidopsis thaliana mutant (atdfb‐3) |
| title_full_unstemmed | Metabolic network changes during skotomorphogenesis in Arabidopsis thaliana mutant (atdfb‐3) |
| title_short | Metabolic network changes during skotomorphogenesis in Arabidopsis thaliana mutant (atdfb‐3) |
| title_sort | metabolic network changes during skotomorphogenesis in arabidopsis thaliana mutant atdfb 3 |
| topic | Arabidopsis folate metabolic networks skotomorphogenesis |
| url | https://doi.org/10.1002/pld3.467 |
| work_keys_str_mv | AT xingjuanli metabolicnetworkchangesduringskotomorphogenesisinarabidopsisthalianamutantatdfb3 AT hongyanmeng metabolicnetworkchangesduringskotomorphogenesisinarabidopsisthalianamutantatdfb3 AT liqingliu metabolicnetworkchangesduringskotomorphogenesisinarabidopsisthalianamutantatdfb3 AT cuiyunhong metabolicnetworkchangesduringskotomorphogenesisinarabidopsisthalianamutantatdfb3 AT chunyizhang metabolicnetworkchangesduringskotomorphogenesisinarabidopsisthalianamutantatdfb3 |