Spermidine Induces Expression of Stress Associated Proteins (SAPs) Genes and Protects Rice Seed from Heat Stress-Induced Damage during Grain-Filling
Heat stress during seed maturation significantly reduced seed size and quality. Polyamines, especially spermidine (Spd), were reported to be closely related to seed development and plant heat tolerance. Stress-associated proteins (SAPs) also played a critical role in plant heat resistance, but the r...
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MDPI AG
2021-09-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/10/10/1544 |
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| author | Min Chen Yuying Fu Qingshan Mou Jianyu An Xiaobo Zhu Temoor Ahmed Sheng Zhang Farwa Basit Jin Hu Yajing Guan |
| author_facet | Min Chen Yuying Fu Qingshan Mou Jianyu An Xiaobo Zhu Temoor Ahmed Sheng Zhang Farwa Basit Jin Hu Yajing Guan |
| author_sort | Min Chen |
| collection | DOAJ |
| description | Heat stress during seed maturation significantly reduced seed size and quality. Polyamines, especially spermidine (Spd), were reported to be closely related to seed development and plant heat tolerance. Stress-associated proteins (SAPs) also played a critical role in plant heat resistance, but the relationship between Spd and SAPs in improving rice tolerance to heat stress during grain filling has not been reported. Our results showed that the external spraying Spd (1.5 mM) significantly increased seed germination rate, germination index, vigor index and 1000-grain weight, significantly increased endogenous Spd, spermine (Spm) content and peroxidase activity; significantly reduced MDA content; and greatly alleviated the impact of heat stress on rice seed quality during grain filling stage as compared with high temperature control. <i>OsSAP5</i> was the most upregulated expression induced by Spd, and may be mainly involved in the Spd-mediated enhancement of high-temperature resistance during rice seed development. Overexpression of <i>OsSAP5</i> in <i>Arabidopsis</i> enhanced 1000-grain weight and seed heat resistance. Exogenous Spd alleviated the survival rate and seedling length, reduced MDA content, and upregulated the expression levels of <i>SPDS</i> and <i>SPMS</i> in <i>Atsap4</i> mutant under high temperature during seed germination. In all, exogenous Spd alleviated the heat damage on seed quality during the grain filling stage and seed germination stage by improving endogenous Spd and Spm. <i>OsSAP5</i>, a key gene induced by Spd, might be involved in the rice heat resistance and seed quality in coordination with Spd and Spm. |
| first_indexed | 2024-03-10T06:46:11Z |
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| language | English |
| last_indexed | 2024-03-10T06:46:11Z |
| publishDate | 2021-09-01 |
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| series | Antioxidants |
| spelling | doaj.art-527064b3afe042b3b6510e1e2002b2692023-11-22T17:15:36ZengMDPI AGAntioxidants2076-39212021-09-011010154410.3390/antiox10101544Spermidine Induces Expression of Stress Associated Proteins (SAPs) Genes and Protects Rice Seed from Heat Stress-Induced Damage during Grain-FillingMin Chen0Yuying Fu1Qingshan Mou2Jianyu An3Xiaobo Zhu4Temoor Ahmed5Sheng Zhang6Farwa Basit7Jin Hu8Yajing Guan9Institute of Crop Sciences, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, ChinaInstitute of Horticulture, Anhui Academy of Agricultural Sciences, Hefei 230041, ChinaInstitute of Crop Sciences, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, ChinaInstitute of Crop Sciences, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, ChinaHainan Research Institute, Zhejiang University, Sanya 572025, ChinaState Key Laboratory of Rice Biology, Institute of Biotechnology, Zhejiang University, Hangzhou 310058, ChinaTaizhou Agricultural Technology Extension Center, Taizhou 318000, ChinaInstitute of Crop Sciences, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, ChinaInstitute of Crop Sciences, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, ChinaInstitute of Crop Sciences, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, ChinaHeat stress during seed maturation significantly reduced seed size and quality. Polyamines, especially spermidine (Spd), were reported to be closely related to seed development and plant heat tolerance. Stress-associated proteins (SAPs) also played a critical role in plant heat resistance, but the relationship between Spd and SAPs in improving rice tolerance to heat stress during grain filling has not been reported. Our results showed that the external spraying Spd (1.5 mM) significantly increased seed germination rate, germination index, vigor index and 1000-grain weight, significantly increased endogenous Spd, spermine (Spm) content and peroxidase activity; significantly reduced MDA content; and greatly alleviated the impact of heat stress on rice seed quality during grain filling stage as compared with high temperature control. <i>OsSAP5</i> was the most upregulated expression induced by Spd, and may be mainly involved in the Spd-mediated enhancement of high-temperature resistance during rice seed development. Overexpression of <i>OsSAP5</i> in <i>Arabidopsis</i> enhanced 1000-grain weight and seed heat resistance. Exogenous Spd alleviated the survival rate and seedling length, reduced MDA content, and upregulated the expression levels of <i>SPDS</i> and <i>SPMS</i> in <i>Atsap4</i> mutant under high temperature during seed germination. In all, exogenous Spd alleviated the heat damage on seed quality during the grain filling stage and seed germination stage by improving endogenous Spd and Spm. <i>OsSAP5</i>, a key gene induced by Spd, might be involved in the rice heat resistance and seed quality in coordination with Spd and Spm.https://www.mdpi.com/2076-3921/10/10/1544high-temperaturespermidineseed qualitystress-associated proteinsthermotolerance |
| spellingShingle | Min Chen Yuying Fu Qingshan Mou Jianyu An Xiaobo Zhu Temoor Ahmed Sheng Zhang Farwa Basit Jin Hu Yajing Guan Spermidine Induces Expression of Stress Associated Proteins (SAPs) Genes and Protects Rice Seed from Heat Stress-Induced Damage during Grain-Filling Antioxidants high-temperature spermidine seed quality stress-associated proteins thermotolerance |
| title | Spermidine Induces Expression of Stress Associated Proteins (SAPs) Genes and Protects Rice Seed from Heat Stress-Induced Damage during Grain-Filling |
| title_full | Spermidine Induces Expression of Stress Associated Proteins (SAPs) Genes and Protects Rice Seed from Heat Stress-Induced Damage during Grain-Filling |
| title_fullStr | Spermidine Induces Expression of Stress Associated Proteins (SAPs) Genes and Protects Rice Seed from Heat Stress-Induced Damage during Grain-Filling |
| title_full_unstemmed | Spermidine Induces Expression of Stress Associated Proteins (SAPs) Genes and Protects Rice Seed from Heat Stress-Induced Damage during Grain-Filling |
| title_short | Spermidine Induces Expression of Stress Associated Proteins (SAPs) Genes and Protects Rice Seed from Heat Stress-Induced Damage during Grain-Filling |
| title_sort | spermidine induces expression of stress associated proteins saps genes and protects rice seed from heat stress induced damage during grain filling |
| topic | high-temperature spermidine seed quality stress-associated proteins thermotolerance |
| url | https://www.mdpi.com/2076-3921/10/10/1544 |
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