Longitudinal section cell morphology of Chinese fir roots and the relationship between root structure and function
IntroductionThe longitudinal section cell morphology of Chinese fir roots was studied to better understand the relationship between root structure and root function.MaterialsIn this study, the adjusted microwave paraffin section method and the selected two sample transparency methods were used to pr...
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Frontiers Media S.A.
2023-02-01
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| Series: | Frontiers in Ecology and Evolution |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fevo.2023.1122860/full |
| _version_ | 1811170408540405760 |
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| author | Linxin Li Linxin Li Ting Zhang Ting Zhang Jingquan Lin Xiaoqian Lian Xiaoqian Lian Xianhua Zou Xiangqing Ma Xiangqing Ma Pengfei Wu Pengfei Wu |
| author_facet | Linxin Li Linxin Li Ting Zhang Ting Zhang Jingquan Lin Xiaoqian Lian Xiaoqian Lian Xianhua Zou Xiangqing Ma Xiangqing Ma Pengfei Wu Pengfei Wu |
| author_sort | Linxin Li |
| collection | DOAJ |
| description | IntroductionThe longitudinal section cell morphology of Chinese fir roots was studied to better understand the relationship between root structure and root function.MaterialsIn this study, the adjusted microwave paraffin section method and the selected two sample transparency methods were used to process the Chinese fir roots and combined with the laser scanning confocal microscopy (LSCM) technique, the morphology of Chinese fir roots longitudinal section can be clearly observed in a short time. At the same time, the observation effect of the longitudinal section cell morphology of the LSCM image of the thick section of the Chinese fir roots and the ordinary optical imaging of the thin section was analyzed and compared.Results and DiscussionThe results showed that: (1) There were apparent differences in the observation effect of cell morphology in longitudinal sections of Chinese fir roots obtained using various treatment methods. Under LSCM, the section with a thickness of 20 μm generated by the microwave paraffin section technique displayed complete cell morphology and clear structure in the root cap, meristem zone, and elongation zone. The overall imaging effect was good; the thickness was 0.42–1.01, 0.64–1.57, and 0.95–2.71 mm, respectively. The cell arrangement in maturation zone cells was more regular. (2) Compared to the ordinary optical imaging of thin sections, the thick sections of roots made by the microwave paraffin section method shortened the time to obtain high-quality sections to ensure the observation effect. Therefore, adopting the microwave paraffin cutting approach to produce thicker root sections under LSCM allows for rapid observation of the cell morphology in longitudinal sections of Chinese fir roots. The current study provides the efficient operation procedure for the microscopic observation technology of the longitudinal section of Chinese fir roots, which is not only beneficial to reveal the relationship between the root structure and function from the microscopic point of view but also provides a technical reference for the anatomical study of other organs and the observation of the longitudinal section cell morphology of plant roots with similar structural characteristics. |
| first_indexed | 2024-04-10T16:56:16Z |
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| id | doaj.art-5276e80872d842a2ab46acb010699190 |
| institution | Directory Open Access Journal |
| issn | 2296-701X |
| language | English |
| last_indexed | 2024-04-10T16:56:16Z |
| publishDate | 2023-02-01 |
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| series | Frontiers in Ecology and Evolution |
| spelling | doaj.art-5276e80872d842a2ab46acb0106991902023-02-07T06:05:23ZengFrontiers Media S.A.Frontiers in Ecology and Evolution2296-701X2023-02-011110.3389/fevo.2023.11228601122860Longitudinal section cell morphology of Chinese fir roots and the relationship between root structure and functionLinxin Li0Linxin Li1Ting Zhang2Ting Zhang3Jingquan Lin4Xiaoqian Lian5Xiaoqian Lian6Xianhua Zou7Xiangqing Ma8Xiangqing Ma9Pengfei Wu10Pengfei Wu11College of Forestry, Fujian Agriculture and Forestry University, Fuzhou, ChinaChinese Fir Engineering Technology Research Center of the State Forestry and Grassland Administration, Fujian Agriculture and Forestry University, Fuzhou, Fujian, ChinaCollege of Forestry, Fujian Agriculture and Forestry University, Fuzhou, ChinaChinese Fir Engineering Technology Research Center of the State Forestry and Grassland Administration, Fujian Agriculture and Forestry University, Fuzhou, Fujian, ChinaFujian Shanghang Baisha Forestry Farm, Shanghang, Fujian, ChinaCollege of Forestry, Fujian Agriculture and Forestry University, Fuzhou, ChinaChinese Fir Engineering Technology Research Center of the State Forestry and Grassland Administration, Fujian Agriculture and Forestry University, Fuzhou, Fujian, ChinaChinese Fir Engineering Technology Research Center of the State Forestry and Grassland Administration, Fujian Agriculture and Forestry University, Fuzhou, Fujian, ChinaCollege of Forestry, Fujian Agriculture and Forestry University, Fuzhou, ChinaChinese Fir Engineering Technology Research Center of the State Forestry and Grassland Administration, Fujian Agriculture and Forestry University, Fuzhou, Fujian, ChinaCollege of Forestry, Fujian Agriculture and Forestry University, Fuzhou, ChinaChinese Fir Engineering Technology Research Center of the State Forestry and Grassland Administration, Fujian Agriculture and Forestry University, Fuzhou, Fujian, ChinaIntroductionThe longitudinal section cell morphology of Chinese fir roots was studied to better understand the relationship between root structure and root function.MaterialsIn this study, the adjusted microwave paraffin section method and the selected two sample transparency methods were used to process the Chinese fir roots and combined with the laser scanning confocal microscopy (LSCM) technique, the morphology of Chinese fir roots longitudinal section can be clearly observed in a short time. At the same time, the observation effect of the longitudinal section cell morphology of the LSCM image of the thick section of the Chinese fir roots and the ordinary optical imaging of the thin section was analyzed and compared.Results and DiscussionThe results showed that: (1) There were apparent differences in the observation effect of cell morphology in longitudinal sections of Chinese fir roots obtained using various treatment methods. Under LSCM, the section with a thickness of 20 μm generated by the microwave paraffin section technique displayed complete cell morphology and clear structure in the root cap, meristem zone, and elongation zone. The overall imaging effect was good; the thickness was 0.42–1.01, 0.64–1.57, and 0.95–2.71 mm, respectively. The cell arrangement in maturation zone cells was more regular. (2) Compared to the ordinary optical imaging of thin sections, the thick sections of roots made by the microwave paraffin section method shortened the time to obtain high-quality sections to ensure the observation effect. Therefore, adopting the microwave paraffin cutting approach to produce thicker root sections under LSCM allows for rapid observation of the cell morphology in longitudinal sections of Chinese fir roots. The current study provides the efficient operation procedure for the microscopic observation technology of the longitudinal section of Chinese fir roots, which is not only beneficial to reveal the relationship between the root structure and function from the microscopic point of view but also provides a technical reference for the anatomical study of other organs and the observation of the longitudinal section cell morphology of plant roots with similar structural characteristics.https://www.frontiersin.org/articles/10.3389/fevo.2023.1122860/fullCunninghamia lanceolataroot tissuecell morphologymicrowave paraffin sectionslaser scanning confocal microscope |
| spellingShingle | Linxin Li Linxin Li Ting Zhang Ting Zhang Jingquan Lin Xiaoqian Lian Xiaoqian Lian Xianhua Zou Xiangqing Ma Xiangqing Ma Pengfei Wu Pengfei Wu Longitudinal section cell morphology of Chinese fir roots and the relationship between root structure and function Frontiers in Ecology and Evolution Cunninghamia lanceolata root tissue cell morphology microwave paraffin sections laser scanning confocal microscope |
| title | Longitudinal section cell morphology of Chinese fir roots and the relationship between root structure and function |
| title_full | Longitudinal section cell morphology of Chinese fir roots and the relationship between root structure and function |
| title_fullStr | Longitudinal section cell morphology of Chinese fir roots and the relationship between root structure and function |
| title_full_unstemmed | Longitudinal section cell morphology of Chinese fir roots and the relationship between root structure and function |
| title_short | Longitudinal section cell morphology of Chinese fir roots and the relationship between root structure and function |
| title_sort | longitudinal section cell morphology of chinese fir roots and the relationship between root structure and function |
| topic | Cunninghamia lanceolata root tissue cell morphology microwave paraffin sections laser scanning confocal microscope |
| url | https://www.frontiersin.org/articles/10.3389/fevo.2023.1122860/full |
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