The Anticancer Effect of Arctium lappa and Glycyrrhiza glabra on HT-29 Colon Cancer and MCF-7 Breast Cancer Cell Lines
Objectives: Breast and colon cancer are the most common malignancy in the world and the common causes of mortality in Western societies. The aim of the current study was to examine the effectiveness of Glycyrrhiza glabra and Arctium extracts on breast cancer (MCF7) and gastrointestinal cancer (HT 29...
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| Format: | Article |
| Language: | English |
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Aras Part Medical International Press
2018-04-01
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| Series: | Crescent Journal of Medical and Biological Sciences |
| Subjects: | |
| Online Access: | http://cjmb.org/uploads/pdf/pdf_CJMB_177.pdf |
| _version_ | 1818758674905038848 |
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| author | Shiva Alizadeh Nazmi Alireza Nourazarian Reza Bahhaj Fatemeh Khakikhatibi |
| author_facet | Shiva Alizadeh Nazmi Alireza Nourazarian Reza Bahhaj Fatemeh Khakikhatibi |
| author_sort | Shiva Alizadeh Nazmi |
| collection | DOAJ |
| description | Objectives: Breast and colon cancer are the most common malignancy in the world and the common causes of mortality in Western societies. The aim of the current study was to examine the effectiveness of Glycyrrhiza glabra and Arctium extracts on breast cancer (MCF7) and gastrointestinal cancer (HT 29) cell lines, with regard to their naturalness, low cost and easy public access.
Materials and Methods: In this study, dose and time were considered as variables in order to determine the optimal effects of the drug. Two MCF7- and HT29 cell lines were cultured on a single layer in RPMI 1640 culture medium and MTT method and flow cytometric method were used to examine apoptosis.
Results: The study of cell survival using MTT showed that the aqueous extract of G. glabra at a dose of 2000 μg/mL and incubation for 24 hours had the most inhibitory effect on the MCF7- cell line. In addition, a dose of 2000 μg/mL of G. glabra aqueous extract with -24hour incubation represents apoptosis by flow cytometric method.
Conclusions: The results of this study confirm the effectualness of this herbal supplement for the treatment of breast cancer, and the inclusion of this herbal supplement in the diet may be effective in treating breast cancer, given the naturalness of the product, its low cost and public availability. |
| first_indexed | 2024-12-18T06:30:35Z |
| format | Article |
| id | doaj.art-5279942dc9844668b66c3967d0f5c922 |
| institution | Directory Open Access Journal |
| issn | 2148-9696 2148-9696 |
| language | English |
| last_indexed | 2024-12-18T06:30:35Z |
| publishDate | 2018-04-01 |
| publisher | Aras Part Medical International Press |
| record_format | Article |
| series | Crescent Journal of Medical and Biological Sciences |
| spelling | doaj.art-5279942dc9844668b66c3967d0f5c9222022-12-21T21:17:54ZengAras Part Medical International PressCrescent Journal of Medical and Biological Sciences2148-96962148-96962018-04-0152133137The Anticancer Effect of Arctium lappa and Glycyrrhiza glabra on HT-29 Colon Cancer and MCF-7 Breast Cancer Cell LinesShiva Alizadeh Nazmi0 Alireza Nourazarian1Reza Bahhaj2Fatemeh Khakikhatibi3Department of Toxicology, Ahar Branch, Islamic Azad University, Ahar, IranDepartment of Biochemistry and Clinical Laboratories, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iranepartment of Parasitology and Mycology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, IranDrug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, IranObjectives: Breast and colon cancer are the most common malignancy in the world and the common causes of mortality in Western societies. The aim of the current study was to examine the effectiveness of Glycyrrhiza glabra and Arctium extracts on breast cancer (MCF7) and gastrointestinal cancer (HT 29) cell lines, with regard to their naturalness, low cost and easy public access. Materials and Methods: In this study, dose and time were considered as variables in order to determine the optimal effects of the drug. Two MCF7- and HT29 cell lines were cultured on a single layer in RPMI 1640 culture medium and MTT method and flow cytometric method were used to examine apoptosis. Results: The study of cell survival using MTT showed that the aqueous extract of G. glabra at a dose of 2000 μg/mL and incubation for 24 hours had the most inhibitory effect on the MCF7- cell line. In addition, a dose of 2000 μg/mL of G. glabra aqueous extract with -24hour incubation represents apoptosis by flow cytometric method. Conclusions: The results of this study confirm the effectualness of this herbal supplement for the treatment of breast cancer, and the inclusion of this herbal supplement in the diet may be effective in treating breast cancer, given the naturalness of the product, its low cost and public availability.http://cjmb.org/uploads/pdf/pdf_CJMB_177.pdfArctiumGlycyrrhizaColonic NeoplasmBreast Neoplasm |
| spellingShingle | Shiva Alizadeh Nazmi Alireza Nourazarian Reza Bahhaj Fatemeh Khakikhatibi The Anticancer Effect of Arctium lappa and Glycyrrhiza glabra on HT-29 Colon Cancer and MCF-7 Breast Cancer Cell Lines Crescent Journal of Medical and Biological Sciences Arctium Glycyrrhiza Colonic Neoplasm Breast Neoplasm |
| title | The Anticancer Effect of Arctium lappa and Glycyrrhiza glabra on HT-29 Colon Cancer and MCF-7 Breast Cancer Cell Lines |
| title_full | The Anticancer Effect of Arctium lappa and Glycyrrhiza glabra on HT-29 Colon Cancer and MCF-7 Breast Cancer Cell Lines |
| title_fullStr | The Anticancer Effect of Arctium lappa and Glycyrrhiza glabra on HT-29 Colon Cancer and MCF-7 Breast Cancer Cell Lines |
| title_full_unstemmed | The Anticancer Effect of Arctium lappa and Glycyrrhiza glabra on HT-29 Colon Cancer and MCF-7 Breast Cancer Cell Lines |
| title_short | The Anticancer Effect of Arctium lappa and Glycyrrhiza glabra on HT-29 Colon Cancer and MCF-7 Breast Cancer Cell Lines |
| title_sort | anticancer effect of arctium lappa and glycyrrhiza glabra on ht 29 colon cancer and mcf 7 breast cancer cell lines |
| topic | Arctium Glycyrrhiza Colonic Neoplasm Breast Neoplasm |
| url | http://cjmb.org/uploads/pdf/pdf_CJMB_177.pdf |
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