Analysis of reference genes stability and histidine kinase expression under cold stress in Cordyceps militaris.
The development of fungal fruiting bodies from a hyphal thallus is inducible under low temperature (cold stress). The molecular mechanism has been subject to surprisingly few studies. Analysis of gene expression level has become an important means to study gene function and its regulation mechanism....
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2020-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0236898 |
| _version_ | 1818933455393652736 |
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| author | Yong-Nan Liu Bi-Yang Liu You-Chu Ma Hai-Long Yang Gao-Qiang Liu |
| author_facet | Yong-Nan Liu Bi-Yang Liu You-Chu Ma Hai-Long Yang Gao-Qiang Liu |
| author_sort | Yong-Nan Liu |
| collection | DOAJ |
| description | The development of fungal fruiting bodies from a hyphal thallus is inducible under low temperature (cold stress). The molecular mechanism has been subject to surprisingly few studies. Analysis of gene expression level has become an important means to study gene function and its regulation mechanism. But identification of reference genes (RGs) stability under cold stress have not been reported in famous medicinal mushroom-forming fungi Cordyceps militaris. Herein, 12 candidate RGs had been systematically validated under cold stress in C. militaris. Three different algorithms, geNorm, NormFinder and BestKeeper were applied to evaluate the expression stability of the RGs. Our results showed that UBC and UBQ were the most stable RGs for cold treatments in short and long periods, respectively. 2 RGs (UBC and PP2A) and 3 RGs (UBQ, TUB and CYP) were the suitable RGs for cold treatments in short and long periods, respectively. Moreover, target genes, two-component-system histidine kinase genes, were selected to validate the most and least stable RGs under cold treatment, which indicated that use of unstable expressed genes as RGs leads to biased results. Our results provide a good starting point for accurate reverse transcriptase quantitative polymerase chain reaction normalization by using UBC and UBQ in C. militaris under cold stress and better support for understanding the mechanism of response to cold stress and fruiting body formation in C. militaris and other mushroom-forming fungi in future research. |
| first_indexed | 2024-12-20T04:48:39Z |
| format | Article |
| id | doaj.art-527b904facee4291b2d303516b9d18fb |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-20T04:48:39Z |
| publishDate | 2020-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-527b904facee4291b2d303516b9d18fb2022-12-21T19:52:54ZengPublic Library of Science (PLoS)PLoS ONE1932-62032020-01-01158e023689810.1371/journal.pone.0236898Analysis of reference genes stability and histidine kinase expression under cold stress in Cordyceps militaris.Yong-Nan LiuBi-Yang LiuYou-Chu MaHai-Long YangGao-Qiang LiuThe development of fungal fruiting bodies from a hyphal thallus is inducible under low temperature (cold stress). The molecular mechanism has been subject to surprisingly few studies. Analysis of gene expression level has become an important means to study gene function and its regulation mechanism. But identification of reference genes (RGs) stability under cold stress have not been reported in famous medicinal mushroom-forming fungi Cordyceps militaris. Herein, 12 candidate RGs had been systematically validated under cold stress in C. militaris. Three different algorithms, geNorm, NormFinder and BestKeeper were applied to evaluate the expression stability of the RGs. Our results showed that UBC and UBQ were the most stable RGs for cold treatments in short and long periods, respectively. 2 RGs (UBC and PP2A) and 3 RGs (UBQ, TUB and CYP) were the suitable RGs for cold treatments in short and long periods, respectively. Moreover, target genes, two-component-system histidine kinase genes, were selected to validate the most and least stable RGs under cold treatment, which indicated that use of unstable expressed genes as RGs leads to biased results. Our results provide a good starting point for accurate reverse transcriptase quantitative polymerase chain reaction normalization by using UBC and UBQ in C. militaris under cold stress and better support for understanding the mechanism of response to cold stress and fruiting body formation in C. militaris and other mushroom-forming fungi in future research.https://doi.org/10.1371/journal.pone.0236898 |
| spellingShingle | Yong-Nan Liu Bi-Yang Liu You-Chu Ma Hai-Long Yang Gao-Qiang Liu Analysis of reference genes stability and histidine kinase expression under cold stress in Cordyceps militaris. PLoS ONE |
| title | Analysis of reference genes stability and histidine kinase expression under cold stress in Cordyceps militaris. |
| title_full | Analysis of reference genes stability and histidine kinase expression under cold stress in Cordyceps militaris. |
| title_fullStr | Analysis of reference genes stability and histidine kinase expression under cold stress in Cordyceps militaris. |
| title_full_unstemmed | Analysis of reference genes stability and histidine kinase expression under cold stress in Cordyceps militaris. |
| title_short | Analysis of reference genes stability and histidine kinase expression under cold stress in Cordyceps militaris. |
| title_sort | analysis of reference genes stability and histidine kinase expression under cold stress in cordyceps militaris |
| url | https://doi.org/10.1371/journal.pone.0236898 |
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