Clinico-pathological and Immunological Changes in Chickens Infected with Chicken Anemia Virus
Chicken infectious anemia virus (CIAV) is an immunosuppressive viral disease causing high economic losses in poultry industry. In this study, 20 farms were represented for a prevalence study of the disease in Ismailia governorate, Egypt.ON532690.1 and ON532691.1 Isolates from bone marrow, thymus lo...
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| Format: | Article |
| Language: | English |
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Assiut University
2023-06-01
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| Series: | Journal of Advanced Veterinary Research |
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| Online Access: | https://advetresearch.com/index.php/AVR/article/view/1231 |
| _version_ | 1797657043677478912 |
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| author | Neven M Ramzy Farida H Mohamed Hala N Ibrahim Asmaa M Badawy Mohamed Fawzy |
| author_facet | Neven M Ramzy Farida H Mohamed Hala N Ibrahim Asmaa M Badawy Mohamed Fawzy |
| author_sort | Neven M Ramzy |
| collection | DOAJ |
| description |
Chicken infectious anemia virus (CIAV) is an immunosuppressive viral disease causing high economic losses in poultry industry. In this study, 20 farms were represented for a prevalence study of the disease in Ismailia governorate, Egypt.ON532690.1 and ON532691.1 Isolates from bone marrow, thymus loops, liver, and spleen of broiler farms exhibiting some clinical and postmortem signs were used for reverse transcription polymerase chain reaction assay. RT-PCR was used to amplify a 418bp product of the CIAV VP1 gene. Three farms out of 20 (15%) were positive. Phylogenetic tree of partial vp1 amino acids were classified into three groups according to change in H/22/N-Q amino acid indicated that there are three CIAV different strains circulating in Egypt. Hematological investigation revealed significant decrease in RBCs count, hemoglobin concentration, and packed cell volume declared normocytic normochromic anemia.The immunological studies revealed a significant decrease in serum lysozyme, nitric oxide (NO), antioxidants (CAT and GSH), total protein,and in the majority of serum protein fractions in infected chickens (G2) compared to apparently healthy (G1) while there were marked increase in G2 than G1 in A: Gratio. This result guides to review the vaccination programs against CIAV in Egypt forimproving the immune response against the infection.
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| first_indexed | 2024-03-11T17:38:41Z |
| format | Article |
| id | doaj.art-527ddf580d7c49a5a49d0bcf231db906 |
| institution | Directory Open Access Journal |
| issn | 2090-6269 2090-6277 |
| language | English |
| last_indexed | 2024-03-11T17:38:41Z |
| publishDate | 2023-06-01 |
| publisher | Assiut University |
| record_format | Article |
| series | Journal of Advanced Veterinary Research |
| spelling | doaj.art-527ddf580d7c49a5a49d0bcf231db9062023-10-18T14:14:31ZengAssiut UniversityJournal of Advanced Veterinary Research2090-62692090-62772023-06-01133Clinico-pathological and Immunological Changes in Chickens Infected with Chicken Anemia VirusNeven M Ramzy0Farida H Mohamed1Hala N Ibrahim2Asmaa M Badawy3Mohamed Fawzy4Virology Department, Animal Health Research Institute, Agriculture Research Center, Ismailia Branch, Ismailia, Egypt.Clinical Pathology Department, Animal Health Research Institute, Agriculture Research Center, Ismailia Branch, Ismailia, Egypt.Immunology Department, Animal Health Research Institute, Agriculture Research Center, A.R.C., Dokki, Egypt.Clinical Pathology Department, Animal Health Research Institute, Agriculture Research Center, Ismailia Branch, Ismailia, Egypt.Virology Department, Faculty of Veterinary Medicine, Suez Canal University, Ismailia, Egypt. Chicken infectious anemia virus (CIAV) is an immunosuppressive viral disease causing high economic losses in poultry industry. In this study, 20 farms were represented for a prevalence study of the disease in Ismailia governorate, Egypt.ON532690.1 and ON532691.1 Isolates from bone marrow, thymus loops, liver, and spleen of broiler farms exhibiting some clinical and postmortem signs were used for reverse transcription polymerase chain reaction assay. RT-PCR was used to amplify a 418bp product of the CIAV VP1 gene. Three farms out of 20 (15%) were positive. Phylogenetic tree of partial vp1 amino acids were classified into three groups according to change in H/22/N-Q amino acid indicated that there are three CIAV different strains circulating in Egypt. Hematological investigation revealed significant decrease in RBCs count, hemoglobin concentration, and packed cell volume declared normocytic normochromic anemia.The immunological studies revealed a significant decrease in serum lysozyme, nitric oxide (NO), antioxidants (CAT and GSH), total protein,and in the majority of serum protein fractions in infected chickens (G2) compared to apparently healthy (G1) while there were marked increase in G2 than G1 in A: Gratio. This result guides to review the vaccination programs against CIAV in Egypt forimproving the immune response against the infection. https://advetresearch.com/index.php/AVR/article/view/1231 BroilerCAVPCRImmunity Serum Hematology Buffalo Lactating Reference values |
| spellingShingle | Neven M Ramzy Farida H Mohamed Hala N Ibrahim Asmaa M Badawy Mohamed Fawzy Clinico-pathological and Immunological Changes in Chickens Infected with Chicken Anemia Virus Journal of Advanced Veterinary Research Broiler CAV PCR Immunity Serum Hematology Buffalo Lactating Reference values |
| title | Clinico-pathological and Immunological Changes in Chickens Infected with Chicken Anemia Virus |
| title_full | Clinico-pathological and Immunological Changes in Chickens Infected with Chicken Anemia Virus |
| title_fullStr | Clinico-pathological and Immunological Changes in Chickens Infected with Chicken Anemia Virus |
| title_full_unstemmed | Clinico-pathological and Immunological Changes in Chickens Infected with Chicken Anemia Virus |
| title_short | Clinico-pathological and Immunological Changes in Chickens Infected with Chicken Anemia Virus |
| title_sort | clinico pathological and immunological changes in chickens infected with chicken anemia virus |
| topic | Broiler CAV PCR Immunity Serum Hematology Buffalo Lactating Reference values |
| url | https://advetresearch.com/index.php/AVR/article/view/1231 |
| work_keys_str_mv | AT nevenmramzy clinicopathologicalandimmunologicalchangesinchickensinfectedwithchickenanemiavirus AT faridahmohamed clinicopathologicalandimmunologicalchangesinchickensinfectedwithchickenanemiavirus AT halanibrahim clinicopathologicalandimmunologicalchangesinchickensinfectedwithchickenanemiavirus AT asmaambadawy clinicopathologicalandimmunologicalchangesinchickensinfectedwithchickenanemiavirus AT mohamedfawzy clinicopathologicalandimmunologicalchangesinchickensinfectedwithchickenanemiavirus |