Antimicrobial resistance patterns and biofilm formation of coagulase-negative Staphylococcus species isolated from subclinical mastitis cow milk samples submitted to the Onderstepoort Milk Laboratory
Abstract Background Increased prevalence of antimicrobial resistance, treatment failure, and financial losses have been reported in dairy cows with coagulase-negative Staphylococcus (CoNS) clinical mastitis, however, studies on CoNS infections are limited in South Africa. Therefore, the objectives o...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2019-11-01
|
| Series: | BMC Veterinary Research |
| Online Access: | http://link.springer.com/article/10.1186/s12917-019-2175-3 |
| _version_ | 1818228798911414272 |
|---|---|
| author | Lufuno Phophi Inge-Marie Petzer Daniel Nenene Qekwana |
| author_facet | Lufuno Phophi Inge-Marie Petzer Daniel Nenene Qekwana |
| author_sort | Lufuno Phophi |
| collection | DOAJ |
| description | Abstract Background Increased prevalence of antimicrobial resistance, treatment failure, and financial losses have been reported in dairy cows with coagulase-negative Staphylococcus (CoNS) clinical mastitis, however, studies on CoNS infections are limited in South Africa. Therefore, the objectives of this study were to investigate the antimicrobial resistance patterns and biofilm formation in CoNS isolated from cow milk samples submitted to the Onderstepoort Milk Laboratory. Results A total of 142 confirmed CoNS isolates were used for this study. Biofilm formation was identified in 18% of CoNS tested. Staphylococcus chromogenes (11%) had the highest proportion of biofilm formation followed by S. haemolyticus (4.0%), S. epidermidis, S. hominis, S. xylosus, and S. simulans with 1% respectively. Ninety percent (90%) of CoNS were resistant to at least one antimicrobial (AMR) and 51% were multidrug-resistant (MDR). Resistance among CoNS was the highest to ampicillin (90%) and penicillin (89%), few isolates resistant to cefoxitin and vancomycin, 9% respectively. Similarly, MDR-S. haemolyticus (44%), MDR-S. epidermidis (65%), and MDR-S. chromogenes (52%) were mainly resistant to penicillins. The most common resistance patterns observed were resistance to penicillin-ampicillin (16%) and penicillin-ampicillin-erythromycin (10%). Only 42% of biofilm positive CoNS were MDR. Conclusion The majority of CoNS in this study were resistance to penicillins. In addition, most isolates were β-lactam resistant and MDR. Biofilm formation among the CoNS in this study was uncommon and there was no significant difference in the proportion of MDR-CoNS based on the ability to form a biofilm. |
| first_indexed | 2024-12-12T10:08:26Z |
| format | Article |
| id | doaj.art-527f578b294c442ba084739595337100 |
| institution | Directory Open Access Journal |
| issn | 1746-6148 |
| language | English |
| last_indexed | 2024-12-12T10:08:26Z |
| publishDate | 2019-11-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Veterinary Research |
| spelling | doaj.art-527f578b294c442ba0847395953371002022-12-22T00:27:52ZengBMCBMC Veterinary Research1746-61482019-11-011511910.1186/s12917-019-2175-3Antimicrobial resistance patterns and biofilm formation of coagulase-negative Staphylococcus species isolated from subclinical mastitis cow milk samples submitted to the Onderstepoort Milk LaboratoryLufuno Phophi0Inge-Marie Petzer1Daniel Nenene Qekwana2Section Veterinary Public Health, Department of Paraclinical Sciences, Faculty of Veterinary Science, University of PretoriaDepartment of Production Animal Studies, Faculty of Veterinary Science, University of PretoriaSection Veterinary Public Health, Department of Paraclinical Sciences, Faculty of Veterinary Science, University of PretoriaAbstract Background Increased prevalence of antimicrobial resistance, treatment failure, and financial losses have been reported in dairy cows with coagulase-negative Staphylococcus (CoNS) clinical mastitis, however, studies on CoNS infections are limited in South Africa. Therefore, the objectives of this study were to investigate the antimicrobial resistance patterns and biofilm formation in CoNS isolated from cow milk samples submitted to the Onderstepoort Milk Laboratory. Results A total of 142 confirmed CoNS isolates were used for this study. Biofilm formation was identified in 18% of CoNS tested. Staphylococcus chromogenes (11%) had the highest proportion of biofilm formation followed by S. haemolyticus (4.0%), S. epidermidis, S. hominis, S. xylosus, and S. simulans with 1% respectively. Ninety percent (90%) of CoNS were resistant to at least one antimicrobial (AMR) and 51% were multidrug-resistant (MDR). Resistance among CoNS was the highest to ampicillin (90%) and penicillin (89%), few isolates resistant to cefoxitin and vancomycin, 9% respectively. Similarly, MDR-S. haemolyticus (44%), MDR-S. epidermidis (65%), and MDR-S. chromogenes (52%) were mainly resistant to penicillins. The most common resistance patterns observed were resistance to penicillin-ampicillin (16%) and penicillin-ampicillin-erythromycin (10%). Only 42% of biofilm positive CoNS were MDR. Conclusion The majority of CoNS in this study were resistance to penicillins. In addition, most isolates were β-lactam resistant and MDR. Biofilm formation among the CoNS in this study was uncommon and there was no significant difference in the proportion of MDR-CoNS based on the ability to form a biofilm.http://link.springer.com/article/10.1186/s12917-019-2175-3 |
| spellingShingle | Lufuno Phophi Inge-Marie Petzer Daniel Nenene Qekwana Antimicrobial resistance patterns and biofilm formation of coagulase-negative Staphylococcus species isolated from subclinical mastitis cow milk samples submitted to the Onderstepoort Milk Laboratory BMC Veterinary Research |
| title | Antimicrobial resistance patterns and biofilm formation of coagulase-negative Staphylococcus species isolated from subclinical mastitis cow milk samples submitted to the Onderstepoort Milk Laboratory |
| title_full | Antimicrobial resistance patterns and biofilm formation of coagulase-negative Staphylococcus species isolated from subclinical mastitis cow milk samples submitted to the Onderstepoort Milk Laboratory |
| title_fullStr | Antimicrobial resistance patterns and biofilm formation of coagulase-negative Staphylococcus species isolated from subclinical mastitis cow milk samples submitted to the Onderstepoort Milk Laboratory |
| title_full_unstemmed | Antimicrobial resistance patterns and biofilm formation of coagulase-negative Staphylococcus species isolated from subclinical mastitis cow milk samples submitted to the Onderstepoort Milk Laboratory |
| title_short | Antimicrobial resistance patterns and biofilm formation of coagulase-negative Staphylococcus species isolated from subclinical mastitis cow milk samples submitted to the Onderstepoort Milk Laboratory |
| title_sort | antimicrobial resistance patterns and biofilm formation of coagulase negative staphylococcus species isolated from subclinical mastitis cow milk samples submitted to the onderstepoort milk laboratory |
| url | http://link.springer.com/article/10.1186/s12917-019-2175-3 |
| work_keys_str_mv | AT lufunophophi antimicrobialresistancepatternsandbiofilmformationofcoagulasenegativestaphylococcusspeciesisolatedfromsubclinicalmastitiscowmilksamplessubmittedtotheonderstepoortmilklaboratory AT ingemariepetzer antimicrobialresistancepatternsandbiofilmformationofcoagulasenegativestaphylococcusspeciesisolatedfromsubclinicalmastitiscowmilksamplessubmittedtotheonderstepoortmilklaboratory AT danielneneneqekwana antimicrobialresistancepatternsandbiofilmformationofcoagulasenegativestaphylococcusspeciesisolatedfromsubclinicalmastitiscowmilksamplessubmittedtotheonderstepoortmilklaboratory |