The impact of local assembly rules on RNA packaging in a T = 1 satellite plant virus.
The vast majority of viruses consist of a nucleic acid surrounded by a protective icosahedral protein shell called the capsid. During viral infection of a host cell, the timing and efficiency of the assembly process is important for ensuring the production of infectious new progeny virus particles....
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2021-08-01
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| Series: | PLoS Computational Biology |
| Online Access: | https://doi.org/10.1371/journal.pcbi.1009306 |
| _version_ | 1797860344252596224 |
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| author | Sam R Hill Reidun Twarock Eric C Dykeman |
| author_facet | Sam R Hill Reidun Twarock Eric C Dykeman |
| author_sort | Sam R Hill |
| collection | DOAJ |
| description | The vast majority of viruses consist of a nucleic acid surrounded by a protective icosahedral protein shell called the capsid. During viral infection of a host cell, the timing and efficiency of the assembly process is important for ensuring the production of infectious new progeny virus particles. In the class of single-stranded RNA (ssRNA) viruses, the assembly of the capsid takes place in tandem with packaging of the ssRNA genome in a highly cooperative co-assembly process. In simple ssRNA viruses such as the bacteriophage MS2 and small RNA plant viruses such as STNV, this cooperative process results from multiple interactions between the protein shell and sites in the RNA genome which have been termed packaging signals. Using a stochastic assembly algorithm which includes cooperative interactions between the protein shell and packaging signals in the RNA genome, we demonstrate that highly efficient assembly of STNV capsids arises from a set of simple local rules. Altering the local assembly rules results in different nucleation scenarios with varying assembly efficiencies, which in some cases depend strongly on interactions with RNA packaging signals. Our results provide a potential simple explanation based on local assembly rules for the ability of some ssRNA viruses to spontaneously assemble around charged polymers and other non-viral RNAs in vitro. |
| first_indexed | 2024-04-09T21:44:21Z |
| format | Article |
| id | doaj.art-52807203e35a4339a203ffa68379a6ab |
| institution | Directory Open Access Journal |
| issn | 1553-734X 1553-7358 |
| language | English |
| last_indexed | 2024-04-09T21:44:21Z |
| publishDate | 2021-08-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Computational Biology |
| spelling | doaj.art-52807203e35a4339a203ffa68379a6ab2023-03-25T05:31:00ZengPublic Library of Science (PLoS)PLoS Computational Biology1553-734X1553-73582021-08-01178e100930610.1371/journal.pcbi.1009306The impact of local assembly rules on RNA packaging in a T = 1 satellite plant virus.Sam R HillReidun TwarockEric C DykemanThe vast majority of viruses consist of a nucleic acid surrounded by a protective icosahedral protein shell called the capsid. During viral infection of a host cell, the timing and efficiency of the assembly process is important for ensuring the production of infectious new progeny virus particles. In the class of single-stranded RNA (ssRNA) viruses, the assembly of the capsid takes place in tandem with packaging of the ssRNA genome in a highly cooperative co-assembly process. In simple ssRNA viruses such as the bacteriophage MS2 and small RNA plant viruses such as STNV, this cooperative process results from multiple interactions between the protein shell and sites in the RNA genome which have been termed packaging signals. Using a stochastic assembly algorithm which includes cooperative interactions between the protein shell and packaging signals in the RNA genome, we demonstrate that highly efficient assembly of STNV capsids arises from a set of simple local rules. Altering the local assembly rules results in different nucleation scenarios with varying assembly efficiencies, which in some cases depend strongly on interactions with RNA packaging signals. Our results provide a potential simple explanation based on local assembly rules for the ability of some ssRNA viruses to spontaneously assemble around charged polymers and other non-viral RNAs in vitro.https://doi.org/10.1371/journal.pcbi.1009306 |
| spellingShingle | Sam R Hill Reidun Twarock Eric C Dykeman The impact of local assembly rules on RNA packaging in a T = 1 satellite plant virus. PLoS Computational Biology |
| title | The impact of local assembly rules on RNA packaging in a T = 1 satellite plant virus. |
| title_full | The impact of local assembly rules on RNA packaging in a T = 1 satellite plant virus. |
| title_fullStr | The impact of local assembly rules on RNA packaging in a T = 1 satellite plant virus. |
| title_full_unstemmed | The impact of local assembly rules on RNA packaging in a T = 1 satellite plant virus. |
| title_short | The impact of local assembly rules on RNA packaging in a T = 1 satellite plant virus. |
| title_sort | impact of local assembly rules on rna packaging in a t 1 satellite plant virus |
| url | https://doi.org/10.1371/journal.pcbi.1009306 |
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