Photoacoustic based evaluation of viscoelastic properties of Gram-negative and Gram-positive bacterial colonies
Abstract Mechanical properties of bacterial colonies are crucial considering both addressing their pathogenic effects and exploring their potential applications. Viscoelasticity is a key mechanical property with major impacts on the cell shapes and functions, which reflects the information about the...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2023-09-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-41663-8 |
| _version_ | 1797452910731198464 |
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| author | Zahra Hosseindokht Mohammadreza Kolahdouz Bahareh Hajikhani Pezhman Sasanpour |
| author_facet | Zahra Hosseindokht Mohammadreza Kolahdouz Bahareh Hajikhani Pezhman Sasanpour |
| author_sort | Zahra Hosseindokht |
| collection | DOAJ |
| description | Abstract Mechanical properties of bacterial colonies are crucial considering both addressing their pathogenic effects and exploring their potential applications. Viscoelasticity is a key mechanical property with major impacts on the cell shapes and functions, which reflects the information about the cell envelope constituents. Hereby, we have proposed the application of photoacoustic viscoelasticity (PAVE) for studying the rheological properties of bacterial colonies. In this regard, we employed an intensity-modulated laser beam as the excitation source followed by the phase delay measurement between the generated PA signal and the reference for the characterization of colonies of two different types of Gram-positive and Gram-negative bacteria. The results of our study show that the colony of Staphylococcus aureus as Gram-positive bacteria has a significantly higher viscoelasticity ratio compared to that value for Acinetobacter baumannii as Gram-negative bacteria (77% difference). This may be due to the differing cell envelope structure between the two species, but we cannot rule out effects of biofilm formation in the colonies. Furthermore, a lumped model has been provided for the mechanical properties of bacterial colonies. |
| first_indexed | 2024-03-09T15:15:30Z |
| format | Article |
| id | doaj.art-5280d7cebb824412bf75f0892b170ced |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-09T15:15:30Z |
| publishDate | 2023-09-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-5280d7cebb824412bf75f0892b170ced2023-11-26T13:05:50ZengNature PortfolioScientific Reports2045-23222023-09-011311810.1038/s41598-023-41663-8Photoacoustic based evaluation of viscoelastic properties of Gram-negative and Gram-positive bacterial coloniesZahra Hosseindokht0Mohammadreza Kolahdouz1Bahareh Hajikhani2Pezhman Sasanpour3School of Electrical and Computer Engineering, College of Engineering, University of TehranSchool of Electrical and Computer Engineering, College of Engineering, University of TehranDepartment of Microbiology, School of Medicine, Shahid Beheshti University of Medical SciencesDepartment of Medical Physics and Biomedical Engineering, School of Medicine, Shahid Beheshti University of Medical SciencesAbstract Mechanical properties of bacterial colonies are crucial considering both addressing their pathogenic effects and exploring their potential applications. Viscoelasticity is a key mechanical property with major impacts on the cell shapes and functions, which reflects the information about the cell envelope constituents. Hereby, we have proposed the application of photoacoustic viscoelasticity (PAVE) for studying the rheological properties of bacterial colonies. In this regard, we employed an intensity-modulated laser beam as the excitation source followed by the phase delay measurement between the generated PA signal and the reference for the characterization of colonies of two different types of Gram-positive and Gram-negative bacteria. The results of our study show that the colony of Staphylococcus aureus as Gram-positive bacteria has a significantly higher viscoelasticity ratio compared to that value for Acinetobacter baumannii as Gram-negative bacteria (77% difference). This may be due to the differing cell envelope structure between the two species, but we cannot rule out effects of biofilm formation in the colonies. Furthermore, a lumped model has been provided for the mechanical properties of bacterial colonies.https://doi.org/10.1038/s41598-023-41663-8 |
| spellingShingle | Zahra Hosseindokht Mohammadreza Kolahdouz Bahareh Hajikhani Pezhman Sasanpour Photoacoustic based evaluation of viscoelastic properties of Gram-negative and Gram-positive bacterial colonies Scientific Reports |
| title | Photoacoustic based evaluation of viscoelastic properties of Gram-negative and Gram-positive bacterial colonies |
| title_full | Photoacoustic based evaluation of viscoelastic properties of Gram-negative and Gram-positive bacterial colonies |
| title_fullStr | Photoacoustic based evaluation of viscoelastic properties of Gram-negative and Gram-positive bacterial colonies |
| title_full_unstemmed | Photoacoustic based evaluation of viscoelastic properties of Gram-negative and Gram-positive bacterial colonies |
| title_short | Photoacoustic based evaluation of viscoelastic properties of Gram-negative and Gram-positive bacterial colonies |
| title_sort | photoacoustic based evaluation of viscoelastic properties of gram negative and gram positive bacterial colonies |
| url | https://doi.org/10.1038/s41598-023-41663-8 |
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