A comparison of radiochemical purity testing of 99m Tc-Pentetate (DTPA) between paper and thin layer chromatographic techniques
Introduction: 99mTc-Pentetate or 99mTc-DTPA (99mTc-diethylenetriaminepentaacetic acid) is radiopharmaceutical material for renal function study in nuclear medicine. To ensure the quality of image, radiochemical purity of radiopharmaceutical material has to be tested before administration to patient....
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| Format: | Article |
| Language: | English |
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Chaing Mai University
2016-09-01
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| Series: | Journal of Associated Medical Sciences |
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| Online Access: | https://www.tci-thaijo.org/index.php/bulletinAMS/article/view/68085 |
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| author | Montree Tungjai Thitima Yingyai Tarika Thumvijit |
| author_facet | Montree Tungjai Thitima Yingyai Tarika Thumvijit |
| author_sort | Montree Tungjai |
| collection | DOAJ |
| description | Introduction: 99mTc-Pentetate or 99mTc-DTPA (99mTc-diethylenetriaminepentaacetic acid) is radiopharmaceutical material for renal function study in nuclear medicine. To ensure the quality of image, radiochemical purity of radiopharmaceutical material has to be tested before administration to patient. Thin layer chromatographic technique is widely used in radiochemical purity testing. Recently, paper chromatography is also recommended because it is cheaper than thin layer chromatography.
Objective: To compare paper chromatographic and thin layer chromatographic techniques on the radiochemical purity testing of 99mTc-DTPA.
Materials and methods: The labeling time for preparing of 99mTc-DTPA were 5, 10 and 30 minutes. Radiochemical purity of 99mTc-DTPA was determined by using two chromatographic techniques. For paper chromatographic technique, a Whatman No.1 paper strip was used as stationary phase, normal saline and acetone as mobile phases. For thin layer chromatographic technique, a thin layer chromatography silica gel (TLC-SG) strip was used as a stationary phase, normal saline and acetone as a mobile phases.
Results: Percentage of radiochemical purity by paper chromatographic technique was 75.8±8.3, 88.3±3.7 and 92.0±3.4 at 5, 10 and 30 minutes of labeling times, respectively. Percentage of radiochemical purity by using thin layer chromatographic technique was 71.1±7.6, 91.2±0.9 and 90.5±0.3 at 5, 10 and 30 minutes of labeling times, respectively. From the study, it was found that percentage of radiochemical purity by using two techniques was not statistically significant difference (p>0.05).
Conclusion: A paper chromatographic and thin layer chromatographic technique was similarly in the radiochemical purity testing of 99mTc-DTPA.
Bull Chiang Mai Assoc Med Sci 2016; 49(2): 307-311. Doi: 10.14456/jams.2016.38 |
| first_indexed | 2024-04-13T23:57:27Z |
| format | Article |
| id | doaj.art-52864f21e35c4544a1fae9290ec448cd |
| institution | Directory Open Access Journal |
| issn | 2539-6056 2539-6056 |
| language | English |
| last_indexed | 2024-04-13T23:57:27Z |
| publishDate | 2016-09-01 |
| publisher | Chaing Mai University |
| record_format | Article |
| series | Journal of Associated Medical Sciences |
| spelling | doaj.art-52864f21e35c4544a1fae9290ec448cd2022-12-22T02:23:49ZengChaing Mai UniversityJournal of Associated Medical Sciences2539-60562539-60562016-09-0149330730768085A comparison of radiochemical purity testing of 99m Tc-Pentetate (DTPA) between paper and thin layer chromatographic techniquesMontree Tungjai0Thitima Yingyai1Tarika Thumvijit2Department of Radiological Technology, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai Province, ThailandDepartment of Radiological Technology, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai Province, ThailandDepartment of Radiological Technology, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai Province, ThailandIntroduction: 99mTc-Pentetate or 99mTc-DTPA (99mTc-diethylenetriaminepentaacetic acid) is radiopharmaceutical material for renal function study in nuclear medicine. To ensure the quality of image, radiochemical purity of radiopharmaceutical material has to be tested before administration to patient. Thin layer chromatographic technique is widely used in radiochemical purity testing. Recently, paper chromatography is also recommended because it is cheaper than thin layer chromatography. Objective: To compare paper chromatographic and thin layer chromatographic techniques on the radiochemical purity testing of 99mTc-DTPA. Materials and methods: The labeling time for preparing of 99mTc-DTPA were 5, 10 and 30 minutes. Radiochemical purity of 99mTc-DTPA was determined by using two chromatographic techniques. For paper chromatographic technique, a Whatman No.1 paper strip was used as stationary phase, normal saline and acetone as mobile phases. For thin layer chromatographic technique, a thin layer chromatography silica gel (TLC-SG) strip was used as a stationary phase, normal saline and acetone as a mobile phases. Results: Percentage of radiochemical purity by paper chromatographic technique was 75.8±8.3, 88.3±3.7 and 92.0±3.4 at 5, 10 and 30 minutes of labeling times, respectively. Percentage of radiochemical purity by using thin layer chromatographic technique was 71.1±7.6, 91.2±0.9 and 90.5±0.3 at 5, 10 and 30 minutes of labeling times, respectively. From the study, it was found that percentage of radiochemical purity by using two techniques was not statistically significant difference (p>0.05). Conclusion: A paper chromatographic and thin layer chromatographic technique was similarly in the radiochemical purity testing of 99mTc-DTPA. Bull Chiang Mai Assoc Med Sci 2016; 49(2): 307-311. Doi: 10.14456/jams.2016.38https://www.tci-thaijo.org/index.php/bulletinAMS/article/view/68085Radiochemical purity99mTc-DTPAchromatography |
| spellingShingle | Montree Tungjai Thitima Yingyai Tarika Thumvijit A comparison of radiochemical purity testing of 99m Tc-Pentetate (DTPA) between paper and thin layer chromatographic techniques Journal of Associated Medical Sciences Radiochemical purity 99mTc-DTPA chromatography |
| title | A comparison of radiochemical purity testing of 99m Tc-Pentetate (DTPA) between paper and thin layer chromatographic techniques |
| title_full | A comparison of radiochemical purity testing of 99m Tc-Pentetate (DTPA) between paper and thin layer chromatographic techniques |
| title_fullStr | A comparison of radiochemical purity testing of 99m Tc-Pentetate (DTPA) between paper and thin layer chromatographic techniques |
| title_full_unstemmed | A comparison of radiochemical purity testing of 99m Tc-Pentetate (DTPA) between paper and thin layer chromatographic techniques |
| title_short | A comparison of radiochemical purity testing of 99m Tc-Pentetate (DTPA) between paper and thin layer chromatographic techniques |
| title_sort | comparison of radiochemical purity testing of 99m tc pentetate dtpa between paper and thin layer chromatographic techniques |
| topic | Radiochemical purity 99mTc-DTPA chromatography |
| url | https://www.tci-thaijo.org/index.php/bulletinAMS/article/view/68085 |
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