Differential expression of osteopontin, and osteoprotegerin mRNA in epicardial adipose tissue between patients with severe coronary artery disease and aortic valvular stenosis: association with HDL subclasses
Abstract Background Previous studies suggest a relationship of the epicardial adipose tissue (EAT) with progression and calcification of the atherosclerotic plaque; however, it is unknown if this tissue expresses genes that may participate on these processes and if the expression of these genes is r...
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| Format: | Article |
| Language: | English |
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BMC
2017-08-01
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| Series: | Lipids in Health and Disease |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12944-017-0550-2 |
| _version_ | 1811200879498362880 |
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| author | María Luna-Luna David Cruz-Robles Nydia Ávila-Vanzzini Valentín Herrera-Alarcón Jesús Martínez-Reding Sergio Criales-Vera Julio Sandoval-Zárate Jesús Vargas-Barrón Carlos Martínez-Sánchez Armando Roberto Tovar-Palacio José Manuel Fragoso Elizabeth Carreón-Torres Gilberto Vargas-Alarcón Óscar Pérez-Méndez |
| author_facet | María Luna-Luna David Cruz-Robles Nydia Ávila-Vanzzini Valentín Herrera-Alarcón Jesús Martínez-Reding Sergio Criales-Vera Julio Sandoval-Zárate Jesús Vargas-Barrón Carlos Martínez-Sánchez Armando Roberto Tovar-Palacio José Manuel Fragoso Elizabeth Carreón-Torres Gilberto Vargas-Alarcón Óscar Pérez-Méndez |
| author_sort | María Luna-Luna |
| collection | DOAJ |
| description | Abstract Background Previous studies suggest a relationship of the epicardial adipose tissue (EAT) with progression and calcification of the atherosclerotic plaque; however, it is unknown if this tissue expresses genes that may participate on these processes and if the expression of these genes is regulated by high-density lipoprotein (HDL) subclasses. Methods To explore this possibility, we determined the mRNA expression by qPCR of a pro-calcifying gene (osteopontin (OPN)), and two anti-calcifying genes (osteoprotegerin (OPG) and osteonectin (ON)), in biopsies of EAT obtained from 15 patients with coronary artery disease (CAD) determined by angiography, and 15 patients with diagnostic of aortic valve stenosis but without CAD as control group. We determined the distribution and composition of HDL subclasses by electrophoresis and their statistical relationship with the gene expression in EAT. Results EAT from CAD patients showed a higher expression level of OPN and OPG than control group, whereas ON expression was similar between groups. Large HDL subclasses were cholesterol-poor in CAD patients as estimated by the cholesterol-to-phospholipid ratio. A linear regression model showed an independent association of OPN expression with HDL3a-cholesterol, and OPG expression with the relative proportion of HDL3b protein. Logistic analysis determined that OPN expression was positively associated with the presence of atherosclerotic plaque Conclusion OPN, ON, and OPG genes are transcribed in EAT; to the exception of ON, the level of expression was different in CAD patients and control group, and correlated with some HDL subclasses, suggesting a new role of these lipoproteins. |
| first_indexed | 2024-04-12T02:12:21Z |
| format | Article |
| id | doaj.art-5290c393333f4e3fa162bc5d1e349ee0 |
| institution | Directory Open Access Journal |
| issn | 1476-511X |
| language | English |
| last_indexed | 2024-04-12T02:12:21Z |
| publishDate | 2017-08-01 |
| publisher | BMC |
| record_format | Article |
| series | Lipids in Health and Disease |
| spelling | doaj.art-5290c393333f4e3fa162bc5d1e349ee02022-12-22T03:52:22ZengBMCLipids in Health and Disease1476-511X2017-08-011611810.1186/s12944-017-0550-2Differential expression of osteopontin, and osteoprotegerin mRNA in epicardial adipose tissue between patients with severe coronary artery disease and aortic valvular stenosis: association with HDL subclassesMaría Luna-Luna0David Cruz-Robles1Nydia Ávila-Vanzzini2Valentín Herrera-Alarcón3Jesús Martínez-Reding4Sergio Criales-Vera5Julio Sandoval-Zárate6Jesús Vargas-Barrón7Carlos Martínez-Sánchez8Armando Roberto Tovar-Palacio9José Manuel Fragoso10Elizabeth Carreón-Torres11Gilberto Vargas-Alarcón12Óscar Pérez-Méndez13Department of Molecular Biology, Instituto Nacional de Cardiología “Ignacio Chávez”Department of Molecular Biology, Instituto Nacional de Cardiología “Ignacio Chávez”Department of Echocardiography, Instituto Nacional de Cardiología “Ignacio Chávez”Department of Cardiothoracic Surgery, Instituto Nacional de Cardiología “Ignacio Chávez”Department of Adult Cardiology, Instituto Nacional de Cardiología “Ignacio Chávez”Department of Radiology, Instituto Nacional de Cardiología “Ignacio Chávez”Department of Cardiopulmonary, Instituto Nacional de Cardiología “Ignacio Chávez”Department of Echocardiography, Instituto Nacional de Cardiología “Ignacio Chávez”Department of Emergency, Instituto Nacional de Cardiología “Ignacio Chávez”Department of Physiology of Nutrition, Instituto Nacional de Ciencias Médicas y Nutrición “Salvador Zubirán”Department of Molecular Biology, Instituto Nacional de Cardiología “Ignacio Chávez”Department of Molecular Biology, Instituto Nacional de Cardiología “Ignacio Chávez”Department of Molecular Biology, Instituto Nacional de Cardiología “Ignacio Chávez”Department of Molecular Biology, Instituto Nacional de Cardiología “Ignacio Chávez”Abstract Background Previous studies suggest a relationship of the epicardial adipose tissue (EAT) with progression and calcification of the atherosclerotic plaque; however, it is unknown if this tissue expresses genes that may participate on these processes and if the expression of these genes is regulated by high-density lipoprotein (HDL) subclasses. Methods To explore this possibility, we determined the mRNA expression by qPCR of a pro-calcifying gene (osteopontin (OPN)), and two anti-calcifying genes (osteoprotegerin (OPG) and osteonectin (ON)), in biopsies of EAT obtained from 15 patients with coronary artery disease (CAD) determined by angiography, and 15 patients with diagnostic of aortic valve stenosis but without CAD as control group. We determined the distribution and composition of HDL subclasses by electrophoresis and their statistical relationship with the gene expression in EAT. Results EAT from CAD patients showed a higher expression level of OPN and OPG than control group, whereas ON expression was similar between groups. Large HDL subclasses were cholesterol-poor in CAD patients as estimated by the cholesterol-to-phospholipid ratio. A linear regression model showed an independent association of OPN expression with HDL3a-cholesterol, and OPG expression with the relative proportion of HDL3b protein. Logistic analysis determined that OPN expression was positively associated with the presence of atherosclerotic plaque Conclusion OPN, ON, and OPG genes are transcribed in EAT; to the exception of ON, the level of expression was different in CAD patients and control group, and correlated with some HDL subclasses, suggesting a new role of these lipoproteins.http://link.springer.com/article/10.1186/s12944-017-0550-2Adipose tissueHigh-density lipoproteinOsteopontinOsteonectinOsteoprotegerin |
| spellingShingle | María Luna-Luna David Cruz-Robles Nydia Ávila-Vanzzini Valentín Herrera-Alarcón Jesús Martínez-Reding Sergio Criales-Vera Julio Sandoval-Zárate Jesús Vargas-Barrón Carlos Martínez-Sánchez Armando Roberto Tovar-Palacio José Manuel Fragoso Elizabeth Carreón-Torres Gilberto Vargas-Alarcón Óscar Pérez-Méndez Differential expression of osteopontin, and osteoprotegerin mRNA in epicardial adipose tissue between patients with severe coronary artery disease and aortic valvular stenosis: association with HDL subclasses Lipids in Health and Disease Adipose tissue High-density lipoprotein Osteopontin Osteonectin Osteoprotegerin |
| title | Differential expression of osteopontin, and osteoprotegerin mRNA in epicardial adipose tissue between patients with severe coronary artery disease and aortic valvular stenosis: association with HDL subclasses |
| title_full | Differential expression of osteopontin, and osteoprotegerin mRNA in epicardial adipose tissue between patients with severe coronary artery disease and aortic valvular stenosis: association with HDL subclasses |
| title_fullStr | Differential expression of osteopontin, and osteoprotegerin mRNA in epicardial adipose tissue between patients with severe coronary artery disease and aortic valvular stenosis: association with HDL subclasses |
| title_full_unstemmed | Differential expression of osteopontin, and osteoprotegerin mRNA in epicardial adipose tissue between patients with severe coronary artery disease and aortic valvular stenosis: association with HDL subclasses |
| title_short | Differential expression of osteopontin, and osteoprotegerin mRNA in epicardial adipose tissue between patients with severe coronary artery disease and aortic valvular stenosis: association with HDL subclasses |
| title_sort | differential expression of osteopontin and osteoprotegerin mrna in epicardial adipose tissue between patients with severe coronary artery disease and aortic valvular stenosis association with hdl subclasses |
| topic | Adipose tissue High-density lipoprotein Osteopontin Osteonectin Osteoprotegerin |
| url | http://link.springer.com/article/10.1186/s12944-017-0550-2 |
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