Coelenterazine sulfotransferase from Renilla muelleri.
The luciferin sulfokinase (coelenterazine sulfotransferase) of Renilla was previously reported to activate the storage form, luciferyl sulfate (coelenterazine sulfate) to luciferin (coelenterazine), the substrate for the luciferase bioluminescence reaction. The gene coding for the coelenterazine sul...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2022-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0276315 |
| _version_ | 1828140970081779712 |
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| author | George Tzertzinis Brenda Baker Jack Benner Elizabeth Brown Ivan R Corrêa Laurence Ettwiller Colleen McClung Ira Schildkraut |
| author_facet | George Tzertzinis Brenda Baker Jack Benner Elizabeth Brown Ivan R Corrêa Laurence Ettwiller Colleen McClung Ira Schildkraut |
| author_sort | George Tzertzinis |
| collection | DOAJ |
| description | The luciferin sulfokinase (coelenterazine sulfotransferase) of Renilla was previously reported to activate the storage form, luciferyl sulfate (coelenterazine sulfate) to luciferin (coelenterazine), the substrate for the luciferase bioluminescence reaction. The gene coding for the coelenterazine sulfotransferase has not been identified. Here we used a combined proteomic/transcriptomic approach to identify and clone the sulfotransferase cDNA. Multiple isoforms of coelenterazine sulfotransferase were identified from the anthozoan Renilla muelleri by intersecting its transcriptome with the LC-MS/MS derived peptide sequences of coelenterazine sulfotransferase purified from Renilla. Two of the isoforms were expressed in E. coli, purified, and partially characterized. The encoded enzymes display sulfotransferase activity that is comparable to that of the native sulfotransferase isolated from Renilla reniformis that was reported in 1970. The bioluminescent assay for sensitive detection of 3'-phosphoadenosine 5'-phosphate (PAP) using the recombinant sulfotransferase is demonstrated. |
| first_indexed | 2024-04-11T19:17:45Z |
| format | Article |
| id | doaj.art-5290c965638b477199470b5ee873664e |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-11T19:17:45Z |
| publishDate | 2022-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-5290c965638b477199470b5ee873664e2022-12-22T04:07:24ZengPublic Library of Science (PLoS)PLoS ONE1932-62032022-01-011710e027631510.1371/journal.pone.0276315Coelenterazine sulfotransferase from Renilla muelleri.George TzertzinisBrenda BakerJack BennerElizabeth BrownIvan R CorrêaLaurence EttwillerColleen McClungIra SchildkrautThe luciferin sulfokinase (coelenterazine sulfotransferase) of Renilla was previously reported to activate the storage form, luciferyl sulfate (coelenterazine sulfate) to luciferin (coelenterazine), the substrate for the luciferase bioluminescence reaction. The gene coding for the coelenterazine sulfotransferase has not been identified. Here we used a combined proteomic/transcriptomic approach to identify and clone the sulfotransferase cDNA. Multiple isoforms of coelenterazine sulfotransferase were identified from the anthozoan Renilla muelleri by intersecting its transcriptome with the LC-MS/MS derived peptide sequences of coelenterazine sulfotransferase purified from Renilla. Two of the isoforms were expressed in E. coli, purified, and partially characterized. The encoded enzymes display sulfotransferase activity that is comparable to that of the native sulfotransferase isolated from Renilla reniformis that was reported in 1970. The bioluminescent assay for sensitive detection of 3'-phosphoadenosine 5'-phosphate (PAP) using the recombinant sulfotransferase is demonstrated.https://doi.org/10.1371/journal.pone.0276315 |
| spellingShingle | George Tzertzinis Brenda Baker Jack Benner Elizabeth Brown Ivan R Corrêa Laurence Ettwiller Colleen McClung Ira Schildkraut Coelenterazine sulfotransferase from Renilla muelleri. PLoS ONE |
| title | Coelenterazine sulfotransferase from Renilla muelleri. |
| title_full | Coelenterazine sulfotransferase from Renilla muelleri. |
| title_fullStr | Coelenterazine sulfotransferase from Renilla muelleri. |
| title_full_unstemmed | Coelenterazine sulfotransferase from Renilla muelleri. |
| title_short | Coelenterazine sulfotransferase from Renilla muelleri. |
| title_sort | coelenterazine sulfotransferase from renilla muelleri |
| url | https://doi.org/10.1371/journal.pone.0276315 |
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