An Effective Method for Decellularization of Human Foreskin: Implications for Skin Regeneration in Small Wounds
Objective: Acellular matrices of different allogeneic or xenogeneic origins are widely used as structural scaffolds in regenerative medicine. The main goal of this research was to optimize a method for decellularization of foreskin for skinregeneration in small wounds.Materials and Methods: In this...
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| Format: | Article |
| Language: | English |
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Royan Institute (ACECR), Tehran
2022-09-01
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| Series: | Cell Journal |
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| Online Access: | https://www.celljournal.org/article_253340_56753af0c2d573773de649ea58be0aa5.pdf |
| _version_ | 1828111640367726592 |
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| author | Shima Rahmati Ali Jalili Mehdi Dehkordi Michelle Przedborski |
| author_facet | Shima Rahmati Ali Jalili Mehdi Dehkordi Michelle Przedborski |
| author_sort | Shima Rahmati |
| collection | DOAJ |
| description | Objective: Acellular matrices of different allogeneic or xenogeneic origins are widely used as structural scaffolds in regenerative medicine. The main goal of this research was to optimize a method for decellularization of foreskin for skinregeneration in small wounds.Materials and Methods: In this experimental study, the dermal layers of foreskin were divided into two sections and subjected to two different decellularization methods: the sodium dodecyl sulfate method (SDS-M), and our optimizedforeskin decellularization method (OFD-M). A combination of non-ionic detergents and SDS were used to decellularize the foreskin in OFD-M. The histological, morphological, and biomechanical properties of both methods were compared. In addition, human umbilical cord mesenchymal stem cells (hucMSCs) were isolated, and the biocompatibility andrecellularization of both scaffolds by hucMSC were subsequently determined.Results: We observed that OFD-M is an appropriate approach for successful removal of cellular components from the foreskin tissue, without physical disturbance to the acellular matrix. In comparison to SDS-M, this new bioscaffold possesses a fine network containing a high amount of collagen fibers and glycosaminoglycans (GAG) (P≤0.03), is biocompatible and harmless for hucMSC (viability 91.7%), and exhibits a relatively high tensile strength.Conclusion: We found that the extracellular matrix (ECM) structural integrity, the main ECM components, and themechanical properties of the foreskin are well maintained after applying the OFD-M decellularization technique, indicating that the resulting scaffold would be a suitable platform for culturing MSC for skin grafting in small wounds |
| first_indexed | 2024-04-11T11:38:49Z |
| format | Article |
| id | doaj.art-529126c0f2e54ae1a4577a0e3607ca48 |
| institution | Directory Open Access Journal |
| issn | 2228-5806 2228-5814 |
| language | English |
| last_indexed | 2024-04-11T11:38:49Z |
| publishDate | 2022-09-01 |
| publisher | Royan Institute (ACECR), Tehran |
| record_format | Article |
| series | Cell Journal |
| spelling | doaj.art-529126c0f2e54ae1a4577a0e3607ca482022-12-22T04:25:53ZengRoyan Institute (ACECR), TehranCell Journal2228-58062228-58142022-09-0124950651410.22074/cellj.2022.8005253340An Effective Method for Decellularization of Human Foreskin: Implications for Skin Regeneration in Small WoundsShima Rahmati0Ali Jalili1Mehdi Dehkordi2Michelle Przedborski3Cancer and Immunology Research Center, Research Institute for Health Development, Kurdistan University of Medical Sciences, Sanandaj, IranCancer and Immunology Research Center, Research Institute for Health Development, Kurdistan University of Medical Sciences, Sanandaj, IranDepartment of Molecular Medicine, School of Advanced Technologies, Shahrekord University of Medical Sciences, Shahrekord, IranDepartment of Applied Mathematics, University of Waterloo, Waterloo, ON, CanadaObjective: Acellular matrices of different allogeneic or xenogeneic origins are widely used as structural scaffolds in regenerative medicine. The main goal of this research was to optimize a method for decellularization of foreskin for skinregeneration in small wounds.Materials and Methods: In this experimental study, the dermal layers of foreskin were divided into two sections and subjected to two different decellularization methods: the sodium dodecyl sulfate method (SDS-M), and our optimizedforeskin decellularization method (OFD-M). A combination of non-ionic detergents and SDS were used to decellularize the foreskin in OFD-M. The histological, morphological, and biomechanical properties of both methods were compared. In addition, human umbilical cord mesenchymal stem cells (hucMSCs) were isolated, and the biocompatibility andrecellularization of both scaffolds by hucMSC were subsequently determined.Results: We observed that OFD-M is an appropriate approach for successful removal of cellular components from the foreskin tissue, without physical disturbance to the acellular matrix. In comparison to SDS-M, this new bioscaffold possesses a fine network containing a high amount of collagen fibers and glycosaminoglycans (GAG) (P≤0.03), is biocompatible and harmless for hucMSC (viability 91.7%), and exhibits a relatively high tensile strength.Conclusion: We found that the extracellular matrix (ECM) structural integrity, the main ECM components, and themechanical properties of the foreskin are well maintained after applying the OFD-M decellularization technique, indicating that the resulting scaffold would be a suitable platform for culturing MSC for skin grafting in small woundshttps://www.celljournal.org/article_253340_56753af0c2d573773de649ea58be0aa5.pdfdecellularized scaffoldsforeskinmesenchymal stem cell |
| spellingShingle | Shima Rahmati Ali Jalili Mehdi Dehkordi Michelle Przedborski An Effective Method for Decellularization of Human Foreskin: Implications for Skin Regeneration in Small Wounds Cell Journal decellularized scaffolds foreskin mesenchymal stem cell |
| title | An Effective Method for Decellularization of Human Foreskin: Implications for Skin Regeneration in Small Wounds |
| title_full | An Effective Method for Decellularization of Human Foreskin: Implications for Skin Regeneration in Small Wounds |
| title_fullStr | An Effective Method for Decellularization of Human Foreskin: Implications for Skin Regeneration in Small Wounds |
| title_full_unstemmed | An Effective Method for Decellularization of Human Foreskin: Implications for Skin Regeneration in Small Wounds |
| title_short | An Effective Method for Decellularization of Human Foreskin: Implications for Skin Regeneration in Small Wounds |
| title_sort | effective method for decellularization of human foreskin implications for skin regeneration in small wounds |
| topic | decellularized scaffolds foreskin mesenchymal stem cell |
| url | https://www.celljournal.org/article_253340_56753af0c2d573773de649ea58be0aa5.pdf |
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