Inhibition of α-Glucosidase Activity and The Toxicity of Tristaniopsis merguensis Griff. Leaf Extract
Abstract : This study aims to determine the antidiabetic activity and toxicity of the acetone extract of Tristaniopsis merguensis Griff leaf. The antidiabetic test was the α-glucosidase inhibition method, while the toxicity test used the Brine Shrimp Lethality Test (BSLT) method. The acetone extract...
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| Format: | Article |
| Language: | English |
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Faculty of Science and Technology Universitas Islam Negeri Ar-Raniry Banda Aceh
2020-06-01
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| Series: | Elkawnie: Journal of Islamic Science and Technology |
| Subjects: | |
| Online Access: | https://jurnal.ar-raniry.ac.id/index.php/elkawnie/article/view/5732 |
| _version_ | 1811225683739803648 |
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| author | Robby Gus Mahardika Occa Roanisca Fajar Indah Puspita Sari |
| author_facet | Robby Gus Mahardika Occa Roanisca Fajar Indah Puspita Sari |
| author_sort | Robby Gus Mahardika |
| collection | DOAJ |
| description | Abstract : This study aims to determine the antidiabetic activity and toxicity of the acetone extract of Tristaniopsis merguensis Griff leaf. The antidiabetic test was the α-glucosidase inhibition method, while the toxicity test used the Brine Shrimp Lethality Test (BSLT) method. The acetone extract possessed antidiabetic activity with an IC50 value of 8.83 ± 0.31 (μg/mL). This value is not much different from the positive control of quercetin which has an IC50 value of 6.04 ± 0.14 (μg/mL). The characteristics of the FT-IR spectrum of acetone extract showed that Tristaniopsis merguensis leaf has the groups Ar-OH (phenolic), -OH (hydroxyl), C=O (ketone) and C=C (aromatic). Based on the toxicity test, the Tristaniopsis merguensis leaf acetone extract has an LC50 value of 959.25 ppm which means that the acetone extract is toxic. Therefore, the acetone extract of Tristaniopsis merguensis might be the potential agent of antidiabetic.
Abstrak : Penelitian ini bertujuan untuk mengetahui aktivitas antidiabetes dan toksisitas dari ekstrak aseton daun Tristaniopsis merguensis Griff. Uji aktivitas antidiabetes ditentukan berdasarkan metode inhibisi enzim α-glucosidase, sedangkan toksisitas ditentukan berdasarkan metode Brine Shrimp Lethality Test (BSLT). Ekstrak aseton memiliki aktivitas antidiabetes dengan nilai IC50 8,83 ± 0,31 (μg/mL). Nilai ini tidak jauh berbeda dengan kontrol positif quersetin yang memiliki nilai IC50 6,04 ± 0,14 (μg/mL). Karakteristik spektrum FT-IR ekstrak aseton menunjukkan bahwa daun Tristaniopsis merguensis memiliki gugus fungsi Ar-OH (fenolik), -OH (hidroksil), C=O (keton) dan C=C (aromatik). Berdasarkan uji toksisitas, ekstrak aseton daun Tristaniopsis merguensis memiliki nilai LC50 sebesar 959,25 ppm yang berarti bahwa ekstrak aseton bersifat toksik. Oleh karena itu, ekstrak aseton dari Tristaniopsis merguensis berpotensi untuk dijadikan agen antidiabetes. |
| first_indexed | 2024-04-12T09:11:30Z |
| format | Article |
| id | doaj.art-52932347285b478e820ad3538b5be78a |
| institution | Directory Open Access Journal |
| issn | 2460-8920 2460-8912 |
| language | English |
| last_indexed | 2024-04-12T09:11:30Z |
| publishDate | 2020-06-01 |
| publisher | Faculty of Science and Technology Universitas Islam Negeri Ar-Raniry Banda Aceh |
| record_format | Article |
| series | Elkawnie: Journal of Islamic Science and Technology |
| spelling | doaj.art-52932347285b478e820ad3538b5be78a2022-12-22T03:38:57ZengFaculty of Science and Technology Universitas Islam Negeri Ar-Raniry Banda AcehElkawnie: Journal of Islamic Science and Technology2460-89202460-89122020-06-0161677610.22373/ekw.v6i1.57324147Inhibition of α-Glucosidase Activity and The Toxicity of Tristaniopsis merguensis Griff. Leaf ExtractRobby Gus Mahardika0Occa Roanisca1Fajar Indah Puspita Sari2Department of Chemistry, Universitas of Bangka BelitungDepartment of Chemistry, Faculty of Engineering, University of Bangka Belitung, Bangka Belitung, IndonesiaDepartment of Chemistry, Faculty of Engineering, University of Bangka Belitung, Bangka Belitung, Indonesia, fipuspitas@gmail.comAbstract : This study aims to determine the antidiabetic activity and toxicity of the acetone extract of Tristaniopsis merguensis Griff leaf. The antidiabetic test was the α-glucosidase inhibition method, while the toxicity test used the Brine Shrimp Lethality Test (BSLT) method. The acetone extract possessed antidiabetic activity with an IC50 value of 8.83 ± 0.31 (μg/mL). This value is not much different from the positive control of quercetin which has an IC50 value of 6.04 ± 0.14 (μg/mL). The characteristics of the FT-IR spectrum of acetone extract showed that Tristaniopsis merguensis leaf has the groups Ar-OH (phenolic), -OH (hydroxyl), C=O (ketone) and C=C (aromatic). Based on the toxicity test, the Tristaniopsis merguensis leaf acetone extract has an LC50 value of 959.25 ppm which means that the acetone extract is toxic. Therefore, the acetone extract of Tristaniopsis merguensis might be the potential agent of antidiabetic. Abstrak : Penelitian ini bertujuan untuk mengetahui aktivitas antidiabetes dan toksisitas dari ekstrak aseton daun Tristaniopsis merguensis Griff. Uji aktivitas antidiabetes ditentukan berdasarkan metode inhibisi enzim α-glucosidase, sedangkan toksisitas ditentukan berdasarkan metode Brine Shrimp Lethality Test (BSLT). Ekstrak aseton memiliki aktivitas antidiabetes dengan nilai IC50 8,83 ± 0,31 (μg/mL). Nilai ini tidak jauh berbeda dengan kontrol positif quersetin yang memiliki nilai IC50 6,04 ± 0,14 (μg/mL). Karakteristik spektrum FT-IR ekstrak aseton menunjukkan bahwa daun Tristaniopsis merguensis memiliki gugus fungsi Ar-OH (fenolik), -OH (hidroksil), C=O (keton) dan C=C (aromatik). Berdasarkan uji toksisitas, ekstrak aseton daun Tristaniopsis merguensis memiliki nilai LC50 sebesar 959,25 ppm yang berarti bahwa ekstrak aseton bersifat toksik. Oleh karena itu, ekstrak aseton dari Tristaniopsis merguensis berpotensi untuk dijadikan agen antidiabetes.https://jurnal.ar-raniry.ac.id/index.php/elkawnie/article/view/5732inhibition of α-glucosidaseacetone extracttristaniopsis merguensis griff.brine shrimp lethality testbslt |
| spellingShingle | Robby Gus Mahardika Occa Roanisca Fajar Indah Puspita Sari Inhibition of α-Glucosidase Activity and The Toxicity of Tristaniopsis merguensis Griff. Leaf Extract Elkawnie: Journal of Islamic Science and Technology inhibition of α-glucosidase acetone extract tristaniopsis merguensis griff. brine shrimp lethality test bslt |
| title | Inhibition of α-Glucosidase Activity and The Toxicity of Tristaniopsis merguensis Griff. Leaf Extract |
| title_full | Inhibition of α-Glucosidase Activity and The Toxicity of Tristaniopsis merguensis Griff. Leaf Extract |
| title_fullStr | Inhibition of α-Glucosidase Activity and The Toxicity of Tristaniopsis merguensis Griff. Leaf Extract |
| title_full_unstemmed | Inhibition of α-Glucosidase Activity and The Toxicity of Tristaniopsis merguensis Griff. Leaf Extract |
| title_short | Inhibition of α-Glucosidase Activity and The Toxicity of Tristaniopsis merguensis Griff. Leaf Extract |
| title_sort | inhibition of α glucosidase activity and the toxicity of tristaniopsis merguensis griff leaf extract |
| topic | inhibition of α-glucosidase acetone extract tristaniopsis merguensis griff. brine shrimp lethality test bslt |
| url | https://jurnal.ar-raniry.ac.id/index.php/elkawnie/article/view/5732 |
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