Transcriptome dynamics in early in vivo developing and in vitro produced porcine embryos
Abstract Background The transcriptional changes around the time of embryonic genome activation in pre-implantation embryos indicate that this process is highly dynamic. In vitro produced porcine blastocysts are known to be less competent than in vivo developed blastocysts. To understand the conditio...
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| Format: | Article |
| Language: | English |
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BMC
2021-02-01
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| Series: | BMC Genomics |
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| Online Access: | https://doi.org/10.1186/s12864-021-07430-7 |
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| author | Vera A. van der Weijden Meret Schmidhauser Mayuko Kurome Johannes Knubben Veronika L. Flöter Eckhard Wolf Susanne E. Ulbrich |
| author_facet | Vera A. van der Weijden Meret Schmidhauser Mayuko Kurome Johannes Knubben Veronika L. Flöter Eckhard Wolf Susanne E. Ulbrich |
| author_sort | Vera A. van der Weijden |
| collection | DOAJ |
| description | Abstract Background The transcriptional changes around the time of embryonic genome activation in pre-implantation embryos indicate that this process is highly dynamic. In vitro produced porcine blastocysts are known to be less competent than in vivo developed blastocysts. To understand the conditions that compromise developmental competence of in vitro embryos, it is crucial to evaluate the transcriptional profile of porcine embryos during pre-implantation stages. In this study, we investigated the transcriptome dynamics in in vivo developed and in vitro produced 4-cell embryos, morulae and hatched blastocysts. Results In vivo developed and in vitro produced embryos displayed largely similar transcriptome profiles during development. Enriched canonical pathways from the 4-cell to the morula transition that were shared between in vivo developed and in vitro produced embryos included oxidative phosphorylation and EIF2 signaling. The shared canonical pathways from the morula to the hatched blastocyst transition were 14–3-3-mediated signaling, xenobiotic metabolism general signaling pathway, and NRF2-mediated oxidative stress response. The in vivo developed and in vitro produced hatched blastocysts further were compared to identify molecular signaling pathways indicative of lower developmental competence of in vitro produced hatched blastocysts. A higher metabolic rate and expression of the arginine transporter SLC7A1 were found in in vitro produced hatched blastocysts. Conclusions Our findings suggest that embryos with compromised developmental potential are arrested at an early stage of development, while embryos developing to the hatched blastocyst stage display largely similar transcriptome profiles, irrespective of the embryo source. The hatched blastocysts derived from the in vitro fertilization-pipeline showed an enrichment in molecular signaling pathways associated with lower developmental competence, compared to the in vivo developed embryos. |
| first_indexed | 2024-12-16T16:33:07Z |
| format | Article |
| id | doaj.art-5295d79420fb4702a468bf015dd29e9b |
| institution | Directory Open Access Journal |
| issn | 1471-2164 |
| language | English |
| last_indexed | 2024-12-16T16:33:07Z |
| publishDate | 2021-02-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Genomics |
| spelling | doaj.art-5295d79420fb4702a468bf015dd29e9b2022-12-21T22:24:32ZengBMCBMC Genomics1471-21642021-02-0122111310.1186/s12864-021-07430-7Transcriptome dynamics in early in vivo developing and in vitro produced porcine embryosVera A. van der Weijden0Meret Schmidhauser1Mayuko Kurome2Johannes Knubben3Veronika L. Flöter4Eckhard Wolf5Susanne E. Ulbrich6ETH Zurich, Animal Physiology, Institute of Agricultural SciencesETH Zurich, Animal Physiology, Institute of Agricultural SciencesChair for Molecular Animal Breeding and Biotechnology, and Center for Innovative Medical Models (CiMM), LMU MunichPhysiology Weihenstephan, Technical University MunichETH Zurich, Animal Physiology, Institute of Agricultural SciencesChair for Molecular Animal Breeding and Biotechnology, and Center for Innovative Medical Models (CiMM), LMU MunichETH Zurich, Animal Physiology, Institute of Agricultural SciencesAbstract Background The transcriptional changes around the time of embryonic genome activation in pre-implantation embryos indicate that this process is highly dynamic. In vitro produced porcine blastocysts are known to be less competent than in vivo developed blastocysts. To understand the conditions that compromise developmental competence of in vitro embryos, it is crucial to evaluate the transcriptional profile of porcine embryos during pre-implantation stages. In this study, we investigated the transcriptome dynamics in in vivo developed and in vitro produced 4-cell embryos, morulae and hatched blastocysts. Results In vivo developed and in vitro produced embryos displayed largely similar transcriptome profiles during development. Enriched canonical pathways from the 4-cell to the morula transition that were shared between in vivo developed and in vitro produced embryos included oxidative phosphorylation and EIF2 signaling. The shared canonical pathways from the morula to the hatched blastocyst transition were 14–3-3-mediated signaling, xenobiotic metabolism general signaling pathway, and NRF2-mediated oxidative stress response. The in vivo developed and in vitro produced hatched blastocysts further were compared to identify molecular signaling pathways indicative of lower developmental competence of in vitro produced hatched blastocysts. A higher metabolic rate and expression of the arginine transporter SLC7A1 were found in in vitro produced hatched blastocysts. Conclusions Our findings suggest that embryos with compromised developmental potential are arrested at an early stage of development, while embryos developing to the hatched blastocyst stage display largely similar transcriptome profiles, irrespective of the embryo source. The hatched blastocysts derived from the in vitro fertilization-pipeline showed an enrichment in molecular signaling pathways associated with lower developmental competence, compared to the in vivo developed embryos.https://doi.org/10.1186/s12864-021-07430-7TranscriptomicsPorcineEmbryo developmentIn vivo embryo developmentin vitro fertilization |
| spellingShingle | Vera A. van der Weijden Meret Schmidhauser Mayuko Kurome Johannes Knubben Veronika L. Flöter Eckhard Wolf Susanne E. Ulbrich Transcriptome dynamics in early in vivo developing and in vitro produced porcine embryos BMC Genomics Transcriptomics Porcine Embryo development In vivo embryo development in vitro fertilization |
| title | Transcriptome dynamics in early in vivo developing and in vitro produced porcine embryos |
| title_full | Transcriptome dynamics in early in vivo developing and in vitro produced porcine embryos |
| title_fullStr | Transcriptome dynamics in early in vivo developing and in vitro produced porcine embryos |
| title_full_unstemmed | Transcriptome dynamics in early in vivo developing and in vitro produced porcine embryos |
| title_short | Transcriptome dynamics in early in vivo developing and in vitro produced porcine embryos |
| title_sort | transcriptome dynamics in early in vivo developing and in vitro produced porcine embryos |
| topic | Transcriptomics Porcine Embryo development In vivo embryo development in vitro fertilization |
| url | https://doi.org/10.1186/s12864-021-07430-7 |
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