Determination of Multi-Class Antibiotics Residues in Farmed Fish and Shrimp from Sri Lanka by Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry (UPLC-MS/MS)
Antibiotics have been used to control the aquatic environment in both therapeutic and prophylactic ways. Antibiotics are particularly difficult to extract due to their strong interactions with biological matrices. In this study, UPLC-MS/MS method was developed and validated for quantitative confirma...
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MDPI AG
2023-03-01
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| Series: | Fishes |
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| author | G. D. T. M. Jayasinghe Joanna Szpunar Ryszard Lobinski E. M. R. K. B. Edirisinghe |
| author_facet | G. D. T. M. Jayasinghe Joanna Szpunar Ryszard Lobinski E. M. R. K. B. Edirisinghe |
| author_sort | G. D. T. M. Jayasinghe |
| collection | DOAJ |
| description | Antibiotics have been used to control the aquatic environment in both therapeutic and prophylactic ways. Antibiotics are particularly difficult to extract due to their strong interactions with biological matrices. In this study, UPLC-MS/MS method was developed and validated for quantitative confirmatory analysis of multi-class antibiotics residues in fish and shrimp. Fourteen antibiotics belonging to sulphonamides, β-lactams, quinolones, sulfones and macrolides were determined within one chromatographic run. The samples were suspended in 0.1 M HCl, and the analytes were extracted into ethyl acetate. The extracts were defatted with cyclohexane. The limits of quantification (LOQ) ranged from 0.24 to 1.32 µg kg<sup>−1</sup> for fish and 0.42–1.62 µg kg<sup>−1</sup> for shrimp samples. The recoveries ranged from 75 to 105%. The method was applied to the analysis of farmed freshwater Tilapia fish (<i>Oreochromis niloticus</i>) and shrimp (<i>Penaeus monodon</i>) collected in Sri Lanka. Sulfacetamide (4.31 ± 0.70 µg kg<sup>−1</sup>) and sulfamethoxypyridazine (0.75 ± 0.15 µg kg<sup>−1</sup>) were detected in the fish, and sulfapyridine (0.21–0.56 µg kg<sup>−1</sup>) and sulfadoxine (0.35–1.44 µg kg<sup>−1</sup>) were detected in the shrimp samples. The concentrations complied with the EU regulation limits for veterinary drug residues in seafood and did not pose a risk in terms of food safety. |
| first_indexed | 2024-03-11T06:32:53Z |
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| language | English |
| last_indexed | 2024-03-11T06:32:53Z |
| publishDate | 2023-03-01 |
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| series | Fishes |
| spelling | doaj.art-52966fda3b284d87aebac5cac1d15d522023-11-17T11:04:21ZengMDPI AGFishes2410-38882023-03-018315410.3390/fishes8030154Determination of Multi-Class Antibiotics Residues in Farmed Fish and Shrimp from Sri Lanka by Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry (UPLC-MS/MS)G. D. T. M. Jayasinghe0Joanna Szpunar1Ryszard Lobinski2E. M. R. K. B. Edirisinghe3Universite de Pau et des Pays de l’Adour, E2S UPPA, CNRS, IPREM UMR 5254, Hélioparc, 64053 Pau, FranceUniversite de Pau et des Pays de l’Adour, E2S UPPA, CNRS, IPREM UMR 5254, Hélioparc, 64053 Pau, FranceUniversite de Pau et des Pays de l’Adour, E2S UPPA, CNRS, IPREM UMR 5254, Hélioparc, 64053 Pau, FranceDepartment of Chemical Sciences, Faculty of Applied Sciences, Rajarata University of SriLanka, Mihintale 50300, Sri LankaAntibiotics have been used to control the aquatic environment in both therapeutic and prophylactic ways. Antibiotics are particularly difficult to extract due to their strong interactions with biological matrices. In this study, UPLC-MS/MS method was developed and validated for quantitative confirmatory analysis of multi-class antibiotics residues in fish and shrimp. Fourteen antibiotics belonging to sulphonamides, β-lactams, quinolones, sulfones and macrolides were determined within one chromatographic run. The samples were suspended in 0.1 M HCl, and the analytes were extracted into ethyl acetate. The extracts were defatted with cyclohexane. The limits of quantification (LOQ) ranged from 0.24 to 1.32 µg kg<sup>−1</sup> for fish and 0.42–1.62 µg kg<sup>−1</sup> for shrimp samples. The recoveries ranged from 75 to 105%. The method was applied to the analysis of farmed freshwater Tilapia fish (<i>Oreochromis niloticus</i>) and shrimp (<i>Penaeus monodon</i>) collected in Sri Lanka. Sulfacetamide (4.31 ± 0.70 µg kg<sup>−1</sup>) and sulfamethoxypyridazine (0.75 ± 0.15 µg kg<sup>−1</sup>) were detected in the fish, and sulfapyridine (0.21–0.56 µg kg<sup>−1</sup>) and sulfadoxine (0.35–1.44 µg kg<sup>−1</sup>) were detected in the shrimp samples. The concentrations complied with the EU regulation limits for veterinary drug residues in seafood and did not pose a risk in terms of food safety.https://www.mdpi.com/2410-3888/8/3/154antibioticsaquaculturefood safetyLC-MS/MS |
| spellingShingle | G. D. T. M. Jayasinghe Joanna Szpunar Ryszard Lobinski E. M. R. K. B. Edirisinghe Determination of Multi-Class Antibiotics Residues in Farmed Fish and Shrimp from Sri Lanka by Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry (UPLC-MS/MS) Fishes antibiotics aquaculture food safety LC-MS/MS |
| title | Determination of Multi-Class Antibiotics Residues in Farmed Fish and Shrimp from Sri Lanka by Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry (UPLC-MS/MS) |
| title_full | Determination of Multi-Class Antibiotics Residues in Farmed Fish and Shrimp from Sri Lanka by Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry (UPLC-MS/MS) |
| title_fullStr | Determination of Multi-Class Antibiotics Residues in Farmed Fish and Shrimp from Sri Lanka by Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry (UPLC-MS/MS) |
| title_full_unstemmed | Determination of Multi-Class Antibiotics Residues in Farmed Fish and Shrimp from Sri Lanka by Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry (UPLC-MS/MS) |
| title_short | Determination of Multi-Class Antibiotics Residues in Farmed Fish and Shrimp from Sri Lanka by Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry (UPLC-MS/MS) |
| title_sort | determination of multi class antibiotics residues in farmed fish and shrimp from sri lanka by ultra performance liquid chromatography tandem mass spectrometry uplc ms ms |
| topic | antibiotics aquaculture food safety LC-MS/MS |
| url | https://www.mdpi.com/2410-3888/8/3/154 |
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