Nematicidal effect of Raphasatin from Raphanus sativus against Meloidogyne incognita
The nematicidal activity of radish bulb (Raphanus sativus) methanol (RME) and aqueous extracts (RAE) was tested against the second stage (J2) root knot nematode Meloidogyne incognita model. The EC50 of RME after 3 d of J2 immersion in test solutions was 312 ± 65 μg/ml. However, no activity was noted...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Sciendo
2022-11-01
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| Series: | Journal of Nematology |
| Subjects: | |
| Online Access: | https://doi.org/10.2478/jofnem-2022-0050 |
| _version_ | 1797405104160112640 |
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| author | Aissani Nadhem Sebai Hichem |
| author_facet | Aissani Nadhem Sebai Hichem |
| author_sort | Aissani Nadhem |
| collection | DOAJ |
| description | The nematicidal activity of radish bulb (Raphanus sativus) methanol (RME) and aqueous extracts (RAE) was tested against the second stage (J2) root knot nematode Meloidogyne incognita model. The EC50 of RME after 3 d of J2 immersion in test solutions was 312 ± 65 μg/ml. However, no activity was noted for RAE (EC50 > 1,000 μg/ml). The chemical composition analysis of the methanol extract carried out by the GC–MS technique showed that 4-methylthio-3-butenyl isothiocyanate (raphasatin) was the most abundant compound at 20%. This pure compound strongly induced J2 paralysis with an EC50 of 1.3 ± 0.2 μg/ml after 24 hr. Comparison with other 11 selected isothiocyanates with structure similarity showed that the double bond at position 3 of the alkyl side chain is important for nematicidal activity, followed by the sulfur group at position 5 and the benzyl group at position 2. In addition, raphasatin showed the highest nematicidal activity with the corresponding lowest antioxidant activity of about 92 ± 18 μg/ml. In conclusion, the results of this investigation reveal that R. sativus and its major compound raphasatin can be integrated into the pest management system. |
| first_indexed | 2024-03-09T03:04:54Z |
| format | Article |
| id | doaj.art-5296be167c8844a1a3c9d0bdbb170da1 |
| institution | Directory Open Access Journal |
| issn | 2640-396X |
| language | English |
| last_indexed | 2024-03-09T03:04:54Z |
| publishDate | 2022-11-01 |
| publisher | Sciendo |
| record_format | Article |
| series | Journal of Nematology |
| spelling | doaj.art-5296be167c8844a1a3c9d0bdbb170da12023-12-04T08:04:12ZengSciendoJournal of Nematology2640-396X2022-11-015411934194710.2478/jofnem-2022-0050Nematicidal effect of Raphasatin from Raphanus sativus against Meloidogyne incognitaAissani Nadhem0Sebai Hichem1Laboratory of Functional Physiology and Valorization of Bioressources, Higher Institute of Biotechnology of Beja, University of Jendouba, JendoubaTunisiaLaboratory of Functional Physiology and Valorization of Bioressources, Higher Institute of Biotechnology of Beja, University of Jendouba, JendoubaTunisiaThe nematicidal activity of radish bulb (Raphanus sativus) methanol (RME) and aqueous extracts (RAE) was tested against the second stage (J2) root knot nematode Meloidogyne incognita model. The EC50 of RME after 3 d of J2 immersion in test solutions was 312 ± 65 μg/ml. However, no activity was noted for RAE (EC50 > 1,000 μg/ml). The chemical composition analysis of the methanol extract carried out by the GC–MS technique showed that 4-methylthio-3-butenyl isothiocyanate (raphasatin) was the most abundant compound at 20%. This pure compound strongly induced J2 paralysis with an EC50 of 1.3 ± 0.2 μg/ml after 24 hr. Comparison with other 11 selected isothiocyanates with structure similarity showed that the double bond at position 3 of the alkyl side chain is important for nematicidal activity, followed by the sulfur group at position 5 and the benzyl group at position 2. In addition, raphasatin showed the highest nematicidal activity with the corresponding lowest antioxidant activity of about 92 ± 18 μg/ml. In conclusion, the results of this investigation reveal that R. sativus and its major compound raphasatin can be integrated into the pest management system.https://doi.org/10.2478/jofnem-2022-0050dpphisothiocyanatesmeloidogyne incognitaraphanus sativusraphasatin |
| spellingShingle | Aissani Nadhem Sebai Hichem Nematicidal effect of Raphasatin from Raphanus sativus against Meloidogyne incognita Journal of Nematology dpph isothiocyanates meloidogyne incognita raphanus sativus raphasatin |
| title | Nematicidal effect of Raphasatin from Raphanus sativus against Meloidogyne incognita |
| title_full | Nematicidal effect of Raphasatin from Raphanus sativus against Meloidogyne incognita |
| title_fullStr | Nematicidal effect of Raphasatin from Raphanus sativus against Meloidogyne incognita |
| title_full_unstemmed | Nematicidal effect of Raphasatin from Raphanus sativus against Meloidogyne incognita |
| title_short | Nematicidal effect of Raphasatin from Raphanus sativus against Meloidogyne incognita |
| title_sort | nematicidal effect of raphasatin from raphanus sativus against meloidogyne incognita |
| topic | dpph isothiocyanates meloidogyne incognita raphanus sativus raphasatin |
| url | https://doi.org/10.2478/jofnem-2022-0050 |
| work_keys_str_mv | AT aissaninadhem nematicidaleffectofraphasatinfromraphanussativusagainstmeloidogyneincognita AT sebaihichem nematicidaleffectofraphasatinfromraphanussativusagainstmeloidogyneincognita |