No Causal Association Between Adiponectin and the Risk of Rheumatoid Arthritis: A Mendelian Randomization Study

Background: Accumulating evidence from observational studies suggested that circulating adiponectin levels are associated with the risk of rheumatoid arthritis (RA), but the causality remains unknown. We aimed to assess the causal relationship of adiponectin with RA risk.Methods: Based on summary st...

Full description

Bibliographic Details
Main Authors: Hanzhu Chen, Shuai Mi, Jiahao Zhu, Weidong Jin, Yasong Li, Tianle Wang, Yingjun Li, Chunhong Fan
Format: Article
Language:English
Published: Frontiers Media S.A. 2021-09-01
Series:Frontiers in Genetics
Subjects:
Online Access:https://www.frontiersin.org/articles/10.3389/fgene.2021.670282/full
_version_ 1818595431652786176
author Hanzhu Chen
Shuai Mi
Jiahao Zhu
Weidong Jin
Yasong Li
Tianle Wang
Yingjun Li
Chunhong Fan
author_facet Hanzhu Chen
Shuai Mi
Jiahao Zhu
Weidong Jin
Yasong Li
Tianle Wang
Yingjun Li
Chunhong Fan
author_sort Hanzhu Chen
collection DOAJ
description Background: Accumulating evidence from observational studies suggested that circulating adiponectin levels are associated with the risk of rheumatoid arthritis (RA), but the causality remains unknown. We aimed to assess the causal relationship of adiponectin with RA risk.Methods: Based on summary statistics from large-scale genome-wide association studies (GWAS), we quantified the genetic correlation between adiponectin and RA. Then bidirectional Mendelian randomization (MR) analysis was performed to assess the causal relationship. Twenty single-nucleotide polymorphisms (SNPs) associated with adiponectin were selected as instrumental variables from a recent GWAS (n = 67,739). We applied theses SNPs to a large-scale GWAS for RA (14,361 cases and 43,923 controls) with replication using RA data from the FinnGen consortium (6,236 cases and 147,221 controls) and the UK Biobank (5,201 cases and 457,732 controls). The inverse-variance weighted (IVW) and multiple pleiotropy-robust methods were used for two-sample MR analyses.Results: Our analyses showed no significant genetic correlation between circulating adiponectin levels and RA [rG = 0.127, 95% confidence interval (CI): –0.012 to 0.266, P = 0.074]. In MR analyses, genetically predicted adiponectin levels were not significantly associated with the RA risk (odds ratio: 0.98, 95% CI: 0.88–1.09, P = 0.669). In the reverse direction analysis, there is little evidence supporting an association of genetic susceptibility to RA with adiponectin (β: 0.007, 95% CI: –0.003 to 0.018, P = 0.177). Replication analyses and sensitivity analyses using different models yielded consistent results.Conclusions: Our findings provided no evidence to support the causal effect of adiponectin levels on RA risk and of RA on circulating adiponectin levels.
first_indexed 2024-12-16T11:15:55Z
format Article
id doaj.art-5296f38d69c94e06a9dd00ddf97f0052
institution Directory Open Access Journal
issn 1664-8021
language English
last_indexed 2024-12-16T11:15:55Z
publishDate 2021-09-01
publisher Frontiers Media S.A.
record_format Article
series Frontiers in Genetics
spelling doaj.art-5296f38d69c94e06a9dd00ddf97f00522022-12-21T22:33:36ZengFrontiers Media S.A.Frontiers in Genetics1664-80212021-09-011210.3389/fgene.2021.670282670282No Causal Association Between Adiponectin and the Risk of Rheumatoid Arthritis: A Mendelian Randomization StudyHanzhu Chen0Shuai Mi1Jiahao Zhu2Weidong Jin3Yasong Li4Tianle Wang5Yingjun Li6Chunhong Fan7School of Public Health, Hangzhou Medical College, Hangzhou, ChinaSchool of Public Health, Hangzhou Medical College, Hangzhou, ChinaSchool of Public Health, Hangzhou Medical College, Hangzhou, ChinaDepartment of Clinical Laboratory, Zhejiang Provincial People’s Hospital, People’s Hospital of Hangzhou Medical College, Hangzhou, ChinaDepartment of Rheumatology and Immunology, Zhejiang Provincial People’s Hospital, People’s Hospital of Hangzhou Medical College, Hangzhou, ChinaDepartment of Rheumatology and Immunology, Zhejiang Provincial People’s Hospital, People’s Hospital of Hangzhou Medical College, Hangzhou, ChinaSchool of Public Health, Hangzhou Medical College, Hangzhou, ChinaSchool of Public Health, Hangzhou Medical College, Hangzhou, ChinaBackground: Accumulating evidence from observational studies suggested that circulating adiponectin levels are associated with the risk of rheumatoid arthritis (RA), but the causality remains unknown. We aimed to assess the causal relationship of adiponectin with RA risk.Methods: Based on summary statistics from large-scale genome-wide association studies (GWAS), we quantified the genetic correlation between adiponectin and RA. Then bidirectional Mendelian randomization (MR) analysis was performed to assess the causal relationship. Twenty single-nucleotide polymorphisms (SNPs) associated with adiponectin were selected as instrumental variables from a recent GWAS (n = 67,739). We applied theses SNPs to a large-scale GWAS for RA (14,361 cases and 43,923 controls) with replication using RA data from the FinnGen consortium (6,236 cases and 147,221 controls) and the UK Biobank (5,201 cases and 457,732 controls). The inverse-variance weighted (IVW) and multiple pleiotropy-robust methods were used for two-sample MR analyses.Results: Our analyses showed no significant genetic correlation between circulating adiponectin levels and RA [rG = 0.127, 95% confidence interval (CI): –0.012 to 0.266, P = 0.074]. In MR analyses, genetically predicted adiponectin levels were not significantly associated with the RA risk (odds ratio: 0.98, 95% CI: 0.88–1.09, P = 0.669). In the reverse direction analysis, there is little evidence supporting an association of genetic susceptibility to RA with adiponectin (β: 0.007, 95% CI: –0.003 to 0.018, P = 0.177). Replication analyses and sensitivity analyses using different models yielded consistent results.Conclusions: Our findings provided no evidence to support the causal effect of adiponectin levels on RA risk and of RA on circulating adiponectin levels.https://www.frontiersin.org/articles/10.3389/fgene.2021.670282/fullrheumatoid arthritisadiponectincausalityMendelian randomizationbidirectional
spellingShingle Hanzhu Chen
Shuai Mi
Jiahao Zhu
Weidong Jin
Yasong Li
Tianle Wang
Yingjun Li
Chunhong Fan
No Causal Association Between Adiponectin and the Risk of Rheumatoid Arthritis: A Mendelian Randomization Study
Frontiers in Genetics
rheumatoid arthritis
adiponectin
causality
Mendelian randomization
bidirectional
title No Causal Association Between Adiponectin and the Risk of Rheumatoid Arthritis: A Mendelian Randomization Study
title_full No Causal Association Between Adiponectin and the Risk of Rheumatoid Arthritis: A Mendelian Randomization Study
title_fullStr No Causal Association Between Adiponectin and the Risk of Rheumatoid Arthritis: A Mendelian Randomization Study
title_full_unstemmed No Causal Association Between Adiponectin and the Risk of Rheumatoid Arthritis: A Mendelian Randomization Study
title_short No Causal Association Between Adiponectin and the Risk of Rheumatoid Arthritis: A Mendelian Randomization Study
title_sort no causal association between adiponectin and the risk of rheumatoid arthritis a mendelian randomization study
topic rheumatoid arthritis
adiponectin
causality
Mendelian randomization
bidirectional
url https://www.frontiersin.org/articles/10.3389/fgene.2021.670282/full
work_keys_str_mv AT hanzhuchen nocausalassociationbetweenadiponectinandtheriskofrheumatoidarthritisamendelianrandomizationstudy
AT shuaimi nocausalassociationbetweenadiponectinandtheriskofrheumatoidarthritisamendelianrandomizationstudy
AT jiahaozhu nocausalassociationbetweenadiponectinandtheriskofrheumatoidarthritisamendelianrandomizationstudy
AT weidongjin nocausalassociationbetweenadiponectinandtheriskofrheumatoidarthritisamendelianrandomizationstudy
AT yasongli nocausalassociationbetweenadiponectinandtheriskofrheumatoidarthritisamendelianrandomizationstudy
AT tianlewang nocausalassociationbetweenadiponectinandtheriskofrheumatoidarthritisamendelianrandomizationstudy
AT yingjunli nocausalassociationbetweenadiponectinandtheriskofrheumatoidarthritisamendelianrandomizationstudy
AT chunhongfan nocausalassociationbetweenadiponectinandtheriskofrheumatoidarthritisamendelianrandomizationstudy