Beta-lactams susceptibility testing of penicillin-resistant, ampicillin-susceptible Enterococcus faecalis isolates: a comparative assessment of Etest and disk diffusion methods against broth dilution
Abstract This study aimed to evaluate the accuracy of disk diffusion and Etest methods, compared to that of the broth dilution reference method for identifying beta-lactam susceptibilities of Penicillin-Resistant, Ampicillin-Susceptible Enterococcus faecalis (PRASEF) isolates. Fifty-nine PRASEF and...
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| Format: | Article |
| Language: | English |
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BMC
2020-09-01
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| Series: | Annals of Clinical Microbiology and Antimicrobials |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12941-020-00386-8 |
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| author | Natália Conceição Wellington Francisco Rodrigues Kessys Lorrânya Peralta de Oliveira Lucas Emanuel Pinheiro da Silva Laís Rezende Cardoso de Souza Cristina da de Cunha Hueb Barata Oliveira Adriana Gonçalves de Oliveira |
| author_facet | Natália Conceição Wellington Francisco Rodrigues Kessys Lorrânya Peralta de Oliveira Lucas Emanuel Pinheiro da Silva Laís Rezende Cardoso de Souza Cristina da de Cunha Hueb Barata Oliveira Adriana Gonçalves de Oliveira |
| author_sort | Natália Conceição |
| collection | DOAJ |
| description | Abstract This study aimed to evaluate the accuracy of disk diffusion and Etest methods, compared to that of the broth dilution reference method for identifying beta-lactam susceptibilities of Penicillin-Resistant, Ampicillin-Susceptible Enterococcus faecalis (PRASEF) isolates. Fifty-nine PRASEF and 15 Penicillin-Susceptible, Ampicillin-Susceptible E. faecalis (PSASEF) clinical nonrepetitive isolates were evaluated. The effectiveness of five beta-lactams (ampicillin, amoxicillin, imipenem, penicillin, and piperacillin) was tested. All antimicrobial susceptibility tests were performed and interpreted according to the Clinical and Laboratory Standards Institute guidelines. Interpretative discrepancies, such as essential agreement, categorical agreement, and errors, were assessed. The acceptability was ≥ 90% for both categorical agreement and essential agreement. Etest proved to be an accurate method for testing beta-lactam susceptibilities of the emerging PRASEF isolates, disk diffusion presented poor performance, particularly for imipenem and piperacillin. |
| first_indexed | 2024-12-12T19:02:38Z |
| format | Article |
| id | doaj.art-529a485d56b440a1a041e871596255e8 |
| institution | Directory Open Access Journal |
| issn | 1476-0711 |
| language | English |
| last_indexed | 2024-12-12T19:02:38Z |
| publishDate | 2020-09-01 |
| publisher | BMC |
| record_format | Article |
| series | Annals of Clinical Microbiology and Antimicrobials |
| spelling | doaj.art-529a485d56b440a1a041e871596255e82022-12-22T00:15:03ZengBMCAnnals of Clinical Microbiology and Antimicrobials1476-07112020-09-011911510.1186/s12941-020-00386-8Beta-lactams susceptibility testing of penicillin-resistant, ampicillin-susceptible Enterococcus faecalis isolates: a comparative assessment of Etest and disk diffusion methods against broth dilutionNatália Conceição0Wellington Francisco Rodrigues1Kessys Lorrânya Peralta de Oliveira2Lucas Emanuel Pinheiro da Silva3Laís Rezende Cardoso de Souza4Cristina da de Cunha Hueb Barata Oliveira5Adriana Gonçalves de Oliveira6Instituto de Ciências Biológicas e Naturais, Universidade Federal do Triângulo MineiroPrograma de Pós-graduação em Ciências da Saúde, Universidade Federal do Triângulo MineiroInstituto Federal de Educação, Ciência e Tecnologia de RondôniaInstituto de Ciências Biológicas e Naturais, Universidade Federal do Triângulo MineiroInstituto de Ciências Biológicas e Naturais, Universidade Federal do Triângulo MineiroInstituto de Ciências da Saúde, Universidade Federal do Triângulo MineiroInstituto de Ciências Biológicas e Naturais, Universidade Federal do Triângulo MineiroAbstract This study aimed to evaluate the accuracy of disk diffusion and Etest methods, compared to that of the broth dilution reference method for identifying beta-lactam susceptibilities of Penicillin-Resistant, Ampicillin-Susceptible Enterococcus faecalis (PRASEF) isolates. Fifty-nine PRASEF and 15 Penicillin-Susceptible, Ampicillin-Susceptible E. faecalis (PSASEF) clinical nonrepetitive isolates were evaluated. The effectiveness of five beta-lactams (ampicillin, amoxicillin, imipenem, penicillin, and piperacillin) was tested. All antimicrobial susceptibility tests were performed and interpreted according to the Clinical and Laboratory Standards Institute guidelines. Interpretative discrepancies, such as essential agreement, categorical agreement, and errors, were assessed. The acceptability was ≥ 90% for both categorical agreement and essential agreement. Etest proved to be an accurate method for testing beta-lactam susceptibilities of the emerging PRASEF isolates, disk diffusion presented poor performance, particularly for imipenem and piperacillin.http://link.springer.com/article/10.1186/s12941-020-00386-8EnterococciEtestDisk diffusionBeta-lactamsPiperacillinImipenem |
| spellingShingle | Natália Conceição Wellington Francisco Rodrigues Kessys Lorrânya Peralta de Oliveira Lucas Emanuel Pinheiro da Silva Laís Rezende Cardoso de Souza Cristina da de Cunha Hueb Barata Oliveira Adriana Gonçalves de Oliveira Beta-lactams susceptibility testing of penicillin-resistant, ampicillin-susceptible Enterococcus faecalis isolates: a comparative assessment of Etest and disk diffusion methods against broth dilution Annals of Clinical Microbiology and Antimicrobials Enterococci Etest Disk diffusion Beta-lactams Piperacillin Imipenem |
| title | Beta-lactams susceptibility testing of penicillin-resistant, ampicillin-susceptible Enterococcus faecalis isolates: a comparative assessment of Etest and disk diffusion methods against broth dilution |
| title_full | Beta-lactams susceptibility testing of penicillin-resistant, ampicillin-susceptible Enterococcus faecalis isolates: a comparative assessment of Etest and disk diffusion methods against broth dilution |
| title_fullStr | Beta-lactams susceptibility testing of penicillin-resistant, ampicillin-susceptible Enterococcus faecalis isolates: a comparative assessment of Etest and disk diffusion methods against broth dilution |
| title_full_unstemmed | Beta-lactams susceptibility testing of penicillin-resistant, ampicillin-susceptible Enterococcus faecalis isolates: a comparative assessment of Etest and disk diffusion methods against broth dilution |
| title_short | Beta-lactams susceptibility testing of penicillin-resistant, ampicillin-susceptible Enterococcus faecalis isolates: a comparative assessment of Etest and disk diffusion methods against broth dilution |
| title_sort | beta lactams susceptibility testing of penicillin resistant ampicillin susceptible enterococcus faecalis isolates a comparative assessment of etest and disk diffusion methods against broth dilution |
| topic | Enterococci Etest Disk diffusion Beta-lactams Piperacillin Imipenem |
| url | http://link.springer.com/article/10.1186/s12941-020-00386-8 |
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