The Antiproliferation Activity of Ganoderma formosanum Extracts on Prostate Cancer Cells
Androgen-independent prostate cancer accounts for mortality in the world. In this study, various extracts of a medical fungus dubbed Ganoderma formosanum were screened for inhibition of DU145 cells, an androgen-independent prostate cancer cell line. Results demonstrated that both hexane (GF-EH) and...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2020-05-01
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| Series: | Mycobiology |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/12298093.2020.1746064 |
| _version_ | 1819015981288128512 |
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| author | Cheng-Yen Chiang Kai-Di Hsu Yen-Yi Lin Chang-Wei Hsieh Jui-Ming Liu Tze-Ying Lu Kuan-Chen Cheng |
| author_facet | Cheng-Yen Chiang Kai-Di Hsu Yen-Yi Lin Chang-Wei Hsieh Jui-Ming Liu Tze-Ying Lu Kuan-Chen Cheng |
| author_sort | Cheng-Yen Chiang |
| collection | DOAJ |
| description | Androgen-independent prostate cancer accounts for mortality in the world. In this study, various extracts of a medical fungus dubbed Ganoderma formosanum were screened for inhibition of DU145 cells, an androgen-independent prostate cancer cell line. Results demonstrated that both hexane (GF-EH) and butanol (GF-EB) fraction of G. formosanum ethanol extract inhibited DU145 cell viability in a dose-dependent manner. GF-EH induced cell-cycle arrest in G1 phase of DU145 cells via downregulation of cyclin E2 protein expression. In addition, GF-EB triggered extrinsic apoptosis of DU145 cells by activating caspase 3 gene expression resulting in programed cell death. Above all, both GF-EH and GF-EB show lower toxicity to normal human fibroblast cell line compared to DU145 cell, implying that they possess specific drug action on cancer cells. This study provides a molecular basis of G. formosanum extract as a potential ingredient for treatment of androgen-independent prostate cancer. |
| first_indexed | 2024-12-21T02:40:22Z |
| format | Article |
| id | doaj.art-529b019376214089903124f61180adfe |
| institution | Directory Open Access Journal |
| issn | 1229-8093 2092-9323 |
| language | English |
| last_indexed | 2024-12-21T02:40:22Z |
| publishDate | 2020-05-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Mycobiology |
| spelling | doaj.art-529b019376214089903124f61180adfe2022-12-21T19:18:42ZengTaylor & Francis GroupMycobiology1229-80932092-93232020-05-0148321922710.1080/12298093.2020.17460641746064The Antiproliferation Activity of Ganoderma formosanum Extracts on Prostate Cancer CellsCheng-Yen Chiang0Kai-Di Hsu1Yen-Yi Lin2Chang-Wei Hsieh3Jui-Ming Liu4Tze-Ying Lu5Kuan-Chen Cheng6Taoyuan General Hospital, Ministry of Health and WelfareNational Taiwan UniversityNational Taiwan UniversityNational Chung Hsing UniversityTaoyuan General Hospital, Ministry of Health and WelfareTaipei Medical University–Shuang Ho HospitalNational Taiwan UniversityAndrogen-independent prostate cancer accounts for mortality in the world. In this study, various extracts of a medical fungus dubbed Ganoderma formosanum were screened for inhibition of DU145 cells, an androgen-independent prostate cancer cell line. Results demonstrated that both hexane (GF-EH) and butanol (GF-EB) fraction of G. formosanum ethanol extract inhibited DU145 cell viability in a dose-dependent manner. GF-EH induced cell-cycle arrest in G1 phase of DU145 cells via downregulation of cyclin E2 protein expression. In addition, GF-EB triggered extrinsic apoptosis of DU145 cells by activating caspase 3 gene expression resulting in programed cell death. Above all, both GF-EH and GF-EB show lower toxicity to normal human fibroblast cell line compared to DU145 cell, implying that they possess specific drug action on cancer cells. This study provides a molecular basis of G. formosanum extract as a potential ingredient for treatment of androgen-independent prostate cancer.http://dx.doi.org/10.1080/12298093.2020.1746064prostate cancerganoderma formosanumcell cycle arrestcyclinapoptosis |
| spellingShingle | Cheng-Yen Chiang Kai-Di Hsu Yen-Yi Lin Chang-Wei Hsieh Jui-Ming Liu Tze-Ying Lu Kuan-Chen Cheng The Antiproliferation Activity of Ganoderma formosanum Extracts on Prostate Cancer Cells Mycobiology prostate cancer ganoderma formosanum cell cycle arrest cyclin apoptosis |
| title | The Antiproliferation Activity of Ganoderma formosanum Extracts on Prostate Cancer Cells |
| title_full | The Antiproliferation Activity of Ganoderma formosanum Extracts on Prostate Cancer Cells |
| title_fullStr | The Antiproliferation Activity of Ganoderma formosanum Extracts on Prostate Cancer Cells |
| title_full_unstemmed | The Antiproliferation Activity of Ganoderma formosanum Extracts on Prostate Cancer Cells |
| title_short | The Antiproliferation Activity of Ganoderma formosanum Extracts on Prostate Cancer Cells |
| title_sort | antiproliferation activity of ganoderma formosanum extracts on prostate cancer cells |
| topic | prostate cancer ganoderma formosanum cell cycle arrest cyclin apoptosis |
| url | http://dx.doi.org/10.1080/12298093.2020.1746064 |
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