<i>UME6</i> Is Involved in the Suppression of Basal Transcription of ABC Transporters and Drug Resistance in the ρ<sup>+</sup> Cells of <i>Saccharomyces cerevisiae</i>
In <i>Saccharomyces</i><i>cerevisiae</i>, the Rpd3L complex contains a histone deacetylase, Rpd3, and the DNA binding proteins, Ume6 and Ash1, and acts as a transcriptional repressor or activator. We previously showed that <i>RPD3</i> and <i>UME6</i> a...
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| Format: | Article |
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MDPI AG
2022-03-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/10/3/601 |
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| author | Yoichi Yamada |
| author_facet | Yoichi Yamada |
| author_sort | Yoichi Yamada |
| collection | DOAJ |
| description | In <i>Saccharomyces</i><i>cerevisiae</i>, the Rpd3L complex contains a histone deacetylase, Rpd3, and the DNA binding proteins, Ume6 and Ash1, and acts as a transcriptional repressor or activator. We previously showed that <i>RPD3</i> and <i>UME6</i> are required for the activation of <i>PDR5</i>, which encodes a major efflux pump, and pleiotropic drug resistance (PDR) in ρ<sup>0/−</sup> cells, which lack mitochondrial DNA. However, there are inconsistent reports regarding whether <i>RPD3</i> and <i>UME6</i> are required for Pdr5-mediated PDR in ρ<sup>+</sup> cells with mitochondrial DNA. Since <i>PDR5</i> expression or PDR in the ρ<sup>+</sup> cells of the <i>rpd3</i>Δ and <i>ume6</i>Δ mutants have primarily been examined using fermentable media, mixed cultures of ρ<sup>+</sup> and ρ<sup>0/−</sup> cells could be used. Therefore, we examined whether <i>RPD3</i> and <i>UME6</i> are required for basal and drug-induced <i>PDR5</i> transcription and PDR in ρ<sup>+</sup> cells using fermentable and nonfermentable media. <i>UME6</i> suppresses the basal transcription levels of the ABC transporters, including <i>PDR5</i>, and drug resistance in ρ<sup>+</sup> cells independent of the carbon source used in the growth medium. In contrast, <i>RPD3</i> is required for drug resistance but did not interfere with the basal <i>PDR5</i> mRNA levels. <i>UME6</i> is also required for the cycloheximide-induced transcription of <i>PDR5</i> in nonfermentable media but not in fermentable media. |
| first_indexed | 2024-03-09T13:15:25Z |
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| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-09T13:15:25Z |
| publishDate | 2022-03-01 |
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| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-529dd90727b6487fb9b58e85f73b2fc02023-11-30T21:36:44ZengMDPI AGMicroorganisms2076-26072022-03-0110360110.3390/microorganisms10030601<i>UME6</i> Is Involved in the Suppression of Basal Transcription of ABC Transporters and Drug Resistance in the ρ<sup>+</sup> Cells of <i>Saccharomyces cerevisiae</i>Yoichi Yamada0Faculty of Biological Science and Technology, Institute of Science and Engineering, Kanazawa University, Kanazawa 920-1192, JapanIn <i>Saccharomyces</i><i>cerevisiae</i>, the Rpd3L complex contains a histone deacetylase, Rpd3, and the DNA binding proteins, Ume6 and Ash1, and acts as a transcriptional repressor or activator. We previously showed that <i>RPD3</i> and <i>UME6</i> are required for the activation of <i>PDR5</i>, which encodes a major efflux pump, and pleiotropic drug resistance (PDR) in ρ<sup>0/−</sup> cells, which lack mitochondrial DNA. However, there are inconsistent reports regarding whether <i>RPD3</i> and <i>UME6</i> are required for Pdr5-mediated PDR in ρ<sup>+</sup> cells with mitochondrial DNA. Since <i>PDR5</i> expression or PDR in the ρ<sup>+</sup> cells of the <i>rpd3</i>Δ and <i>ume6</i>Δ mutants have primarily been examined using fermentable media, mixed cultures of ρ<sup>+</sup> and ρ<sup>0/−</sup> cells could be used. Therefore, we examined whether <i>RPD3</i> and <i>UME6</i> are required for basal and drug-induced <i>PDR5</i> transcription and PDR in ρ<sup>+</sup> cells using fermentable and nonfermentable media. <i>UME6</i> suppresses the basal transcription levels of the ABC transporters, including <i>PDR5</i>, and drug resistance in ρ<sup>+</sup> cells independent of the carbon source used in the growth medium. In contrast, <i>RPD3</i> is required for drug resistance but did not interfere with the basal <i>PDR5</i> mRNA levels. <i>UME6</i> is also required for the cycloheximide-induced transcription of <i>PDR5</i> in nonfermentable media but not in fermentable media.https://www.mdpi.com/2076-2607/10/3/601<i>Saccharomyces cerevisiae</i><i>UME6</i><i>RPD3</i><i>PDR5</i>pleiotropic drug resistanceρ<sup>+</sup> cells |
| spellingShingle | Yoichi Yamada <i>UME6</i> Is Involved in the Suppression of Basal Transcription of ABC Transporters and Drug Resistance in the ρ<sup>+</sup> Cells of <i>Saccharomyces cerevisiae</i> Microorganisms <i>Saccharomyces cerevisiae</i> <i>UME6</i> <i>RPD3</i> <i>PDR5</i> pleiotropic drug resistance ρ<sup>+</sup> cells |
| title | <i>UME6</i> Is Involved in the Suppression of Basal Transcription of ABC Transporters and Drug Resistance in the ρ<sup>+</sup> Cells of <i>Saccharomyces cerevisiae</i> |
| title_full | <i>UME6</i> Is Involved in the Suppression of Basal Transcription of ABC Transporters and Drug Resistance in the ρ<sup>+</sup> Cells of <i>Saccharomyces cerevisiae</i> |
| title_fullStr | <i>UME6</i> Is Involved in the Suppression of Basal Transcription of ABC Transporters and Drug Resistance in the ρ<sup>+</sup> Cells of <i>Saccharomyces cerevisiae</i> |
| title_full_unstemmed | <i>UME6</i> Is Involved in the Suppression of Basal Transcription of ABC Transporters and Drug Resistance in the ρ<sup>+</sup> Cells of <i>Saccharomyces cerevisiae</i> |
| title_short | <i>UME6</i> Is Involved in the Suppression of Basal Transcription of ABC Transporters and Drug Resistance in the ρ<sup>+</sup> Cells of <i>Saccharomyces cerevisiae</i> |
| title_sort | i ume6 i is involved in the suppression of basal transcription of abc transporters and drug resistance in the ρ sup sup cells of i saccharomyces cerevisiae i |
| topic | <i>Saccharomyces cerevisiae</i> <i>UME6</i> <i>RPD3</i> <i>PDR5</i> pleiotropic drug resistance ρ<sup>+</sup> cells |
| url | https://www.mdpi.com/2076-2607/10/3/601 |
| work_keys_str_mv | AT yoichiyamada iume6iisinvolvedinthesuppressionofbasaltranscriptionofabctransportersanddrugresistanceinthersupsupcellsofisaccharomycescerevisiaei |