Transcriptome Analysis Reveals Genes and Pathways Associated with Salt Tolerance during Seed Germination in <i>Suaeda liaotungensis</i>
Seed germination is susceptible to external environmental factors, especially salt stress. <i>Suaeda liaotungensis</i> is a halophyte with strong salt tolerance, and the germination rate of brown seeds under 1000 mM NaCl treatment still reached 28.9%. To explore the mechanism of salt str...
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MDPI AG
2022-10-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/20/12229 |
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| author | Jieqiong Song Xinjie Liu Xinxin Li Hongfei Wang Ruowen Chu Fangfang Qu Sixue Zhang Qiuli Li |
| author_facet | Jieqiong Song Xinjie Liu Xinxin Li Hongfei Wang Ruowen Chu Fangfang Qu Sixue Zhang Qiuli Li |
| author_sort | Jieqiong Song |
| collection | DOAJ |
| description | Seed germination is susceptible to external environmental factors, especially salt stress. <i>Suaeda liaotungensis</i> is a halophyte with strong salt tolerance, and the germination rate of brown seeds under 1000 mM NaCl treatment still reached 28.9%. To explore the mechanism of salt stress response during brown seed germination in <i>Suaeda liaotungensis</i>, we conducted transcriptomic analysis on the dry seeds (SlD), germinated seeds under the control condition (SlG_C), and salt treatment (SlG_N). Transcriptome analysis revealed that 13314 and 755 differentially expressed genes (DEGs) from SlD vs. SlG_C and SlG_C vs. SlG_N were detected, respectively. Most DEGs were enriched in pathways related to transcription regulation and hormone signal transduction, ROS metabolism, cell wall organization or biogenesis, and carbohydrate metabolic process in two contrasting groups. Compared with the control condition, POD and CAT activity, H<sub>2</sub>O<sub>2</sub>, soluble sugar, and proline contents were increased during germinated seeds under salt stress. Furthermore, functional analysis demonstrated that overexpression of <i>SlNAC2</i> significantly enhanced salt tolerance during the germination stage in <i>Arabidopsis</i>. These results not only revealed the tolerant mechanism of brown seed germination in response to salinity stress but also promoted the exploration and application of salt-tolerant gene resources of <i>Suaeda liaotungensis</i>. |
| first_indexed | 2024-03-09T20:06:40Z |
| format | Article |
| id | doaj.art-52a007c78c8c4e3694c80464182ca02d |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T20:06:40Z |
| publishDate | 2022-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-52a007c78c8c4e3694c80464182ca02d2023-11-24T00:29:38ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-10-0123201222910.3390/ijms232012229Transcriptome Analysis Reveals Genes and Pathways Associated with Salt Tolerance during Seed Germination in <i>Suaeda liaotungensis</i>Jieqiong Song0Xinjie Liu1Xinxin Li2Hongfei Wang3Ruowen Chu4Fangfang Qu5Sixue Zhang6Qiuli Li7Key Laboratory of Plant Biotechnology of Liaoning Province, School of Life Sciences, Liaoning Normal University, Dalian 116081, ChinaKey Laboratory of Plant Biotechnology of Liaoning Province, School of Life Sciences, Liaoning Normal University, Dalian 116081, ChinaKey Laboratory of Plant Biotechnology of Liaoning Province, School of Life Sciences, Liaoning Normal University, Dalian 116081, ChinaKey Laboratory of Plant Biotechnology of Liaoning Province, School of Life Sciences, Liaoning Normal University, Dalian 116081, ChinaKey Laboratory of Plant Biotechnology of Liaoning Province, School of Life Sciences, Liaoning Normal University, Dalian 116081, ChinaKey Laboratory of Plant Biotechnology of Liaoning Province, School of Life Sciences, Liaoning Normal University, Dalian 116081, ChinaKey Laboratory of Plant Biotechnology of Liaoning Province, School of Life Sciences, Liaoning Normal University, Dalian 116081, ChinaKey Laboratory of Plant Biotechnology of Liaoning Province, School of Life Sciences, Liaoning Normal University, Dalian 116081, ChinaSeed germination is susceptible to external environmental factors, especially salt stress. <i>Suaeda liaotungensis</i> is a halophyte with strong salt tolerance, and the germination rate of brown seeds under 1000 mM NaCl treatment still reached 28.9%. To explore the mechanism of salt stress response during brown seed germination in <i>Suaeda liaotungensis</i>, we conducted transcriptomic analysis on the dry seeds (SlD), germinated seeds under the control condition (SlG_C), and salt treatment (SlG_N). Transcriptome analysis revealed that 13314 and 755 differentially expressed genes (DEGs) from SlD vs. SlG_C and SlG_C vs. SlG_N were detected, respectively. Most DEGs were enriched in pathways related to transcription regulation and hormone signal transduction, ROS metabolism, cell wall organization or biogenesis, and carbohydrate metabolic process in two contrasting groups. Compared with the control condition, POD and CAT activity, H<sub>2</sub>O<sub>2</sub>, soluble sugar, and proline contents were increased during germinated seeds under salt stress. Furthermore, functional analysis demonstrated that overexpression of <i>SlNAC2</i> significantly enhanced salt tolerance during the germination stage in <i>Arabidopsis</i>. These results not only revealed the tolerant mechanism of brown seed germination in response to salinity stress but also promoted the exploration and application of salt-tolerant gene resources of <i>Suaeda liaotungensis</i>.https://www.mdpi.com/1422-0067/23/20/12229salt stresshalophyte<i>Suaeda liaotungensis</i>seed germinationtranscriptomic |
| spellingShingle | Jieqiong Song Xinjie Liu Xinxin Li Hongfei Wang Ruowen Chu Fangfang Qu Sixue Zhang Qiuli Li Transcriptome Analysis Reveals Genes and Pathways Associated with Salt Tolerance during Seed Germination in <i>Suaeda liaotungensis</i> International Journal of Molecular Sciences salt stress halophyte <i>Suaeda liaotungensis</i> seed germination transcriptomic |
| title | Transcriptome Analysis Reveals Genes and Pathways Associated with Salt Tolerance during Seed Germination in <i>Suaeda liaotungensis</i> |
| title_full | Transcriptome Analysis Reveals Genes and Pathways Associated with Salt Tolerance during Seed Germination in <i>Suaeda liaotungensis</i> |
| title_fullStr | Transcriptome Analysis Reveals Genes and Pathways Associated with Salt Tolerance during Seed Germination in <i>Suaeda liaotungensis</i> |
| title_full_unstemmed | Transcriptome Analysis Reveals Genes and Pathways Associated with Salt Tolerance during Seed Germination in <i>Suaeda liaotungensis</i> |
| title_short | Transcriptome Analysis Reveals Genes and Pathways Associated with Salt Tolerance during Seed Germination in <i>Suaeda liaotungensis</i> |
| title_sort | transcriptome analysis reveals genes and pathways associated with salt tolerance during seed germination in i suaeda liaotungensis i |
| topic | salt stress halophyte <i>Suaeda liaotungensis</i> seed germination transcriptomic |
| url | https://www.mdpi.com/1422-0067/23/20/12229 |
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