Hemostatic and Tissue Regeneration Performance of Novel Electrospun Chitosan-Based Materials
The application of chitosan (Ch) as a promising biopolymer with hemostatic properties and high biocompatibility is limited due to its prolonged degradation time, which, in turn, slows the repair process. In the present research, we aimed to develop new technologies to reduce the biodegradation time...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-05-01
|
| Series: | Biomedicines |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2227-9059/9/6/588 |
| _version_ | 1827691841914404864 |
|---|---|
| author | Volodymyr Deineka Oksana Sulaieva Mykola Pernakov Viktoriia Korniienko Yevheniia Husak Anna Yanovska Aziza Yusupova Yuliia Tkachenko Oksana Kalinkevich Alena Zlatska Maksym Pogorielov |
| author_facet | Volodymyr Deineka Oksana Sulaieva Mykola Pernakov Viktoriia Korniienko Yevheniia Husak Anna Yanovska Aziza Yusupova Yuliia Tkachenko Oksana Kalinkevich Alena Zlatska Maksym Pogorielov |
| author_sort | Volodymyr Deineka |
| collection | DOAJ |
| description | The application of chitosan (Ch) as a promising biopolymer with hemostatic properties and high biocompatibility is limited due to its prolonged degradation time, which, in turn, slows the repair process. In the present research, we aimed to develop new technologies to reduce the biodegradation time of Ch-based materials for hemostatic application. This study was undertaken to assess the biocompatibility and hemostatic and tissue-regeneration performance of Ch-PEO-copolymer prepared by electrospinning technique. Chitosan electrospinning membranes (ChEsM) were made from Ch and polyethylene oxide (PEO) powders for rich high-porous material with sufficient hemostatic parameters. The structure, porosity, density, antibacterial properties, in vitro degradation and biocompatibility of ChEsM were evaluated and compared to the conventional Ch sponge (ChSp). In addition, the hemostatic and bioactive performance of both materials were examined in vivo, using the liver-bleeding model in rats. A penetrating punch biopsy of the left liver lobe was performed to simulate bleeding from a non-compressible irregular wound. Appropriately shaped ChSp or ChEsM were applied to tissue lesions. Electrospinning allows us to produce high-porous membranes with relevant ChSp degradation and swelling properties. Both materials demonstrated high biocompatibility and hemostatic effectiveness in vitro. However, the antibacterial properties of ChEsM were not as good when compared to the ChSp. In vivo studies confirmed superior ChEsM biocompatibility and sufficient hemostatic performance, with tight interplay with host cells and tissues. The in vivo model showed a higher biodegradation rate of ChEsM and advanced liver repair. |
| first_indexed | 2024-03-10T11:10:15Z |
| format | Article |
| id | doaj.art-52a023b242f746598f6d9520b709266b |
| institution | Directory Open Access Journal |
| issn | 2227-9059 |
| language | English |
| last_indexed | 2024-03-10T11:10:15Z |
| publishDate | 2021-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomedicines |
| spelling | doaj.art-52a023b242f746598f6d9520b709266b2023-11-21T20:48:16ZengMDPI AGBiomedicines2227-90592021-05-019658810.3390/biomedicines9060588Hemostatic and Tissue Regeneration Performance of Novel Electrospun Chitosan-Based MaterialsVolodymyr Deineka0Oksana Sulaieva1Mykola Pernakov2Viktoriia Korniienko3Yevheniia Husak4Anna Yanovska5Aziza Yusupova6Yuliia Tkachenko7Oksana Kalinkevich8Alena Zlatska9Maksym Pogorielov10Medical Institute, Sumy State University, 40007 Sumy, UkraineMedical Institute, Sumy State University, 40007 Sumy, UkraineMedical Institute, Sumy State University, 40007 Sumy, UkraineMedical Institute, Sumy State University, 40007 Sumy, UkraineMedical Institute, Sumy State University, 40007 Sumy, UkraineMedical Institute, Sumy State University, 40007 Sumy, UkraineMedical Institute, Sumy State University, 40007 Sumy, UkraineMedical Institute, Sumy State University, 40007 Sumy, UkraineInstitute of Applied Physics, 40000 Sumy, UkraineBiotechnology Laboratory Ilaya Regeneration, Medical Company Ilaya, 03115 Kyiv, UkraineMedical Institute, Sumy State University, 40007 Sumy, UkraineThe application of chitosan (Ch) as a promising biopolymer with hemostatic properties and high biocompatibility is limited due to its prolonged degradation time, which, in turn, slows the repair process. In the present research, we aimed to develop new technologies to reduce the biodegradation time of Ch-based materials for hemostatic application. This study was undertaken to assess the biocompatibility and hemostatic and tissue-regeneration performance of Ch-PEO-copolymer prepared by electrospinning technique. Chitosan electrospinning membranes (ChEsM) were made from Ch and polyethylene oxide (PEO) powders for rich high-porous material with sufficient hemostatic parameters. The structure, porosity, density, antibacterial properties, in vitro degradation and biocompatibility of ChEsM were evaluated and compared to the conventional Ch sponge (ChSp). In addition, the hemostatic and bioactive performance of both materials were examined in vivo, using the liver-bleeding model in rats. A penetrating punch biopsy of the left liver lobe was performed to simulate bleeding from a non-compressible irregular wound. Appropriately shaped ChSp or ChEsM were applied to tissue lesions. Electrospinning allows us to produce high-porous membranes with relevant ChSp degradation and swelling properties. Both materials demonstrated high biocompatibility and hemostatic effectiveness in vitro. However, the antibacterial properties of ChEsM were not as good when compared to the ChSp. In vivo studies confirmed superior ChEsM biocompatibility and sufficient hemostatic performance, with tight interplay with host cells and tissues. The in vivo model showed a higher biodegradation rate of ChEsM and advanced liver repair.https://www.mdpi.com/2227-9059/9/6/588chitosanelectrospinninghemostasisregeneration |
| spellingShingle | Volodymyr Deineka Oksana Sulaieva Mykola Pernakov Viktoriia Korniienko Yevheniia Husak Anna Yanovska Aziza Yusupova Yuliia Tkachenko Oksana Kalinkevich Alena Zlatska Maksym Pogorielov Hemostatic and Tissue Regeneration Performance of Novel Electrospun Chitosan-Based Materials Biomedicines chitosan electrospinning hemostasis regeneration |
| title | Hemostatic and Tissue Regeneration Performance of Novel Electrospun Chitosan-Based Materials |
| title_full | Hemostatic and Tissue Regeneration Performance of Novel Electrospun Chitosan-Based Materials |
| title_fullStr | Hemostatic and Tissue Regeneration Performance of Novel Electrospun Chitosan-Based Materials |
| title_full_unstemmed | Hemostatic and Tissue Regeneration Performance of Novel Electrospun Chitosan-Based Materials |
| title_short | Hemostatic and Tissue Regeneration Performance of Novel Electrospun Chitosan-Based Materials |
| title_sort | hemostatic and tissue regeneration performance of novel electrospun chitosan based materials |
| topic | chitosan electrospinning hemostasis regeneration |
| url | https://www.mdpi.com/2227-9059/9/6/588 |
| work_keys_str_mv | AT volodymyrdeineka hemostaticandtissueregenerationperformanceofnovelelectrospunchitosanbasedmaterials AT oksanasulaieva hemostaticandtissueregenerationperformanceofnovelelectrospunchitosanbasedmaterials AT mykolapernakov hemostaticandtissueregenerationperformanceofnovelelectrospunchitosanbasedmaterials AT viktoriiakorniienko hemostaticandtissueregenerationperformanceofnovelelectrospunchitosanbasedmaterials AT yevheniiahusak hemostaticandtissueregenerationperformanceofnovelelectrospunchitosanbasedmaterials AT annayanovska hemostaticandtissueregenerationperformanceofnovelelectrospunchitosanbasedmaterials AT azizayusupova hemostaticandtissueregenerationperformanceofnovelelectrospunchitosanbasedmaterials AT yuliiatkachenko hemostaticandtissueregenerationperformanceofnovelelectrospunchitosanbasedmaterials AT oksanakalinkevich hemostaticandtissueregenerationperformanceofnovelelectrospunchitosanbasedmaterials AT alenazlatska hemostaticandtissueregenerationperformanceofnovelelectrospunchitosanbasedmaterials AT maksympogorielov hemostaticandtissueregenerationperformanceofnovelelectrospunchitosanbasedmaterials |