Genotyping on ALDH2: comparison of four different technologies.
<h4>Objectives</h4>This study aimed to compare the accuracy and performance of four genotyping methods for detecting single nucleotide polymorphisms (SNPs) in aldehyde dehydrogenase-2 (ALDH2), which is the principal enzyme involved in alcohol metabolism.<h4>Design and methods</h...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2015-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0122745 |
| _version_ | 1819042669804912640 |
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| author | Lina Zhang Jinzhao Zhao Guanglin Cui Hong Wang Dao Wen Wang |
| author_facet | Lina Zhang Jinzhao Zhao Guanglin Cui Hong Wang Dao Wen Wang |
| author_sort | Lina Zhang |
| collection | DOAJ |
| description | <h4>Objectives</h4>This study aimed to compare the accuracy and performance of four genotyping methods for detecting single nucleotide polymorphisms (SNPs) in aldehyde dehydrogenase-2 (ALDH2), which is the principal enzyme involved in alcohol metabolism.<h4>Design and methods</h4>We genotyped rs671 of ALDH2 in 96 coronary heart disease (CHD) patients with four methods including high resolution melting analysis (HRM), TaqMan allelic discrimination assay (TaqMan), allele-specific PCR (AS-PCR) and pyrosequencing. Meanwhile, we compared the accuracy and performance of these methods.<h4>Results</h4>All selected patients were successfully genotyped with referred methods. The results of these four assays showed 100% concordant results and had 100% accuracy as verified by Sanger sequencing.<h4>Conclusions</h4>All of the referred methods can be used for genotyping ALDH2 rs671 with the same accuracy compared to Sanger sequencing. In small size of clinical samples, HRM and AS-PCR outperform over others due to their lower cost and less hands-on operation, which are suitable for clinical application. |
| first_indexed | 2024-12-21T09:44:34Z |
| format | Article |
| id | doaj.art-52a16c851324439e927cc02c33fbdc69 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-21T09:44:34Z |
| publishDate | 2015-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-52a16c851324439e927cc02c33fbdc692022-12-21T19:08:21ZengPublic Library of Science (PLoS)PLoS ONE1932-62032015-01-01103e012274510.1371/journal.pone.0122745Genotyping on ALDH2: comparison of four different technologies.Lina ZhangJinzhao ZhaoGuanglin CuiHong WangDao Wen Wang<h4>Objectives</h4>This study aimed to compare the accuracy and performance of four genotyping methods for detecting single nucleotide polymorphisms (SNPs) in aldehyde dehydrogenase-2 (ALDH2), which is the principal enzyme involved in alcohol metabolism.<h4>Design and methods</h4>We genotyped rs671 of ALDH2 in 96 coronary heart disease (CHD) patients with four methods including high resolution melting analysis (HRM), TaqMan allelic discrimination assay (TaqMan), allele-specific PCR (AS-PCR) and pyrosequencing. Meanwhile, we compared the accuracy and performance of these methods.<h4>Results</h4>All selected patients were successfully genotyped with referred methods. The results of these four assays showed 100% concordant results and had 100% accuracy as verified by Sanger sequencing.<h4>Conclusions</h4>All of the referred methods can be used for genotyping ALDH2 rs671 with the same accuracy compared to Sanger sequencing. In small size of clinical samples, HRM and AS-PCR outperform over others due to their lower cost and less hands-on operation, which are suitable for clinical application.https://doi.org/10.1371/journal.pone.0122745 |
| spellingShingle | Lina Zhang Jinzhao Zhao Guanglin Cui Hong Wang Dao Wen Wang Genotyping on ALDH2: comparison of four different technologies. PLoS ONE |
| title | Genotyping on ALDH2: comparison of four different technologies. |
| title_full | Genotyping on ALDH2: comparison of four different technologies. |
| title_fullStr | Genotyping on ALDH2: comparison of four different technologies. |
| title_full_unstemmed | Genotyping on ALDH2: comparison of four different technologies. |
| title_short | Genotyping on ALDH2: comparison of four different technologies. |
| title_sort | genotyping on aldh2 comparison of four different technologies |
| url | https://doi.org/10.1371/journal.pone.0122745 |
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