Cardiovascular complications are resolved by tuna protein hydrolysate supplementation in rats fed with a high-fat diet
Abstract This study is aimed to investigate whether tuna protein hydrolysate (TPH) supplementation could alleviate cardiovascular complications induced by a high-fat diet (HFD) in rats. Rats were fed a HFD for 16 weeks and given TPH (100 mg/kg, 300 mg/kg, or 500 mg/kg) or metformin (100 mg/kg) (n = ...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2023-07-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-39538-z |
| _version_ | 1797769566537908224 |
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| author | Putcharawipa Maneesai Jintanaporn Wattanathorn Prapassorn Potue Juthamas Khamseekaew Siwayu Rattanakanokchai Wipawee Thukham-Mee Supaporn Muchimapura Poungrat Pakdeechote |
| author_facet | Putcharawipa Maneesai Jintanaporn Wattanathorn Prapassorn Potue Juthamas Khamseekaew Siwayu Rattanakanokchai Wipawee Thukham-Mee Supaporn Muchimapura Poungrat Pakdeechote |
| author_sort | Putcharawipa Maneesai |
| collection | DOAJ |
| description | Abstract This study is aimed to investigate whether tuna protein hydrolysate (TPH) supplementation could alleviate cardiovascular complications induced by a high-fat diet (HFD) in rats. Rats were fed a HFD for 16 weeks and given TPH (100 mg/kg, 300 mg/kg, or 500 mg/kg) or metformin (100 mg/kg) (n = 8) for the last four weeks. TPH had the following effects: resolved their impaired glucose tolerance, hyperglycemia, dyslipidemia, obesity, and hypertension (p < 0.05); alleviated left ventricular dysfunction and hypertrophy (p < 0.05), and vascular dysfunction and hypertrophy (p < 0.05); adipocyte hypertrophy; increases in circulating leptin and tumor necrosis factor (TNF-α) were mitigated (p < 0.05); increased renin-angiotensin system (RAS), oxidative stress, and decreased nitric oxide metabolites were modulated (p < 0.05). TPH restored the expression of angiotensin II receptor type 1 (AT1R)/NADPH oxidase 2 (NOX2), endothelial nitric oxide synthase (eNOS), nuclear factor erythroid 2-related factor (Nrf2)/heme oxygenase-1 (HO-1), and peroxisome proliferator-activated receptor γ (PPARγ)/the nuclear factor kappa B (NF-κB) protein in cardiovascular tissue (p < 0.05). In metabolic syndrome (MS) rats, metformin and TPH had comparable effects. In conclusion, TPH alleviated cardiovascular complications related to MS. It suppressed RAS, oxidative stress, and inflammation that were associated with modulation of AT1R/NOX2, eNOS, Nrf2/HO-1, and PPARγ/NF-κB expression. |
| first_indexed | 2024-03-12T21:09:58Z |
| format | Article |
| id | doaj.art-52a18e7af479420c87cdc8fc742e7ea9 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-12T21:09:58Z |
| publishDate | 2023-07-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-52a18e7af479420c87cdc8fc742e7ea92023-07-30T11:12:46ZengNature PortfolioScientific Reports2045-23222023-07-0113111710.1038/s41598-023-39538-zCardiovascular complications are resolved by tuna protein hydrolysate supplementation in rats fed with a high-fat dietPutcharawipa Maneesai0Jintanaporn Wattanathorn1Prapassorn Potue2Juthamas Khamseekaew3Siwayu Rattanakanokchai4Wipawee Thukham-Mee5Supaporn Muchimapura6Poungrat Pakdeechote7Department of Physiology, Faculty of Medicine, Khon Kaen UniversityDepartment of Physiology, Faculty of Medicine, Khon Kaen UniversityDepartment of Physiology, Faculty of Medicine, Khon Kaen UniversityDepartment of Physiology, Faculty of Medicine, Khon Kaen UniversityFaculty of Veterinary Medicine, Khon Kaen UniversityDepartment of Physiology, Faculty of Medicine, Khon Kaen UniversityDepartment of Physiology, Faculty of Medicine, Khon Kaen UniversityDepartment of Physiology, Faculty of Medicine, Khon Kaen UniversityAbstract This study is aimed to investigate whether tuna protein hydrolysate (TPH) supplementation could alleviate cardiovascular complications induced by a high-fat diet (HFD) in rats. Rats were fed a HFD for 16 weeks and given TPH (100 mg/kg, 300 mg/kg, or 500 mg/kg) or metformin (100 mg/kg) (n = 8) for the last four weeks. TPH had the following effects: resolved their impaired glucose tolerance, hyperglycemia, dyslipidemia, obesity, and hypertension (p < 0.05); alleviated left ventricular dysfunction and hypertrophy (p < 0.05), and vascular dysfunction and hypertrophy (p < 0.05); adipocyte hypertrophy; increases in circulating leptin and tumor necrosis factor (TNF-α) were mitigated (p < 0.05); increased renin-angiotensin system (RAS), oxidative stress, and decreased nitric oxide metabolites were modulated (p < 0.05). TPH restored the expression of angiotensin II receptor type 1 (AT1R)/NADPH oxidase 2 (NOX2), endothelial nitric oxide synthase (eNOS), nuclear factor erythroid 2-related factor (Nrf2)/heme oxygenase-1 (HO-1), and peroxisome proliferator-activated receptor γ (PPARγ)/the nuclear factor kappa B (NF-κB) protein in cardiovascular tissue (p < 0.05). In metabolic syndrome (MS) rats, metformin and TPH had comparable effects. In conclusion, TPH alleviated cardiovascular complications related to MS. It suppressed RAS, oxidative stress, and inflammation that were associated with modulation of AT1R/NOX2, eNOS, Nrf2/HO-1, and PPARγ/NF-κB expression.https://doi.org/10.1038/s41598-023-39538-z |
| spellingShingle | Putcharawipa Maneesai Jintanaporn Wattanathorn Prapassorn Potue Juthamas Khamseekaew Siwayu Rattanakanokchai Wipawee Thukham-Mee Supaporn Muchimapura Poungrat Pakdeechote Cardiovascular complications are resolved by tuna protein hydrolysate supplementation in rats fed with a high-fat diet Scientific Reports |
| title | Cardiovascular complications are resolved by tuna protein hydrolysate supplementation in rats fed with a high-fat diet |
| title_full | Cardiovascular complications are resolved by tuna protein hydrolysate supplementation in rats fed with a high-fat diet |
| title_fullStr | Cardiovascular complications are resolved by tuna protein hydrolysate supplementation in rats fed with a high-fat diet |
| title_full_unstemmed | Cardiovascular complications are resolved by tuna protein hydrolysate supplementation in rats fed with a high-fat diet |
| title_short | Cardiovascular complications are resolved by tuna protein hydrolysate supplementation in rats fed with a high-fat diet |
| title_sort | cardiovascular complications are resolved by tuna protein hydrolysate supplementation in rats fed with a high fat diet |
| url | https://doi.org/10.1038/s41598-023-39538-z |
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