Chemical Modification of Glycoproteins’ Carbohydrate Moiety as a General Strategy for the Synthesis of Efficient Biocatalysts by Biomimetic Mineralization: The Case of Glucose Oxidase
Zeolitic imidazolate framework-8 (ZIF-8) is widely used as a protective coating to encapsulate proteins via biomimetic mineralization. The formation of nucleation centers and further biocomposite crystal growth is entirely governed by the pure electrostatic interactions between the protein’s surface...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-11-01
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| Series: | Polymers |
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| Online Access: | https://www.mdpi.com/2073-4360/13/22/3875 |
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| author | Marija D. Stanišić Nikolina Popović Kokar Predrag Ristić Ana Marija Balaž Milan Senćanski Miloš Ognjanović Veljko R. Đokić Radivoje Prodanović Tamara R. Todorović |
| author_facet | Marija D. Stanišić Nikolina Popović Kokar Predrag Ristić Ana Marija Balaž Milan Senćanski Miloš Ognjanović Veljko R. Đokić Radivoje Prodanović Tamara R. Todorović |
| author_sort | Marija D. Stanišić |
| collection | DOAJ |
| description | Zeolitic imidazolate framework-8 (ZIF-8) is widely used as a protective coating to encapsulate proteins via biomimetic mineralization. The formation of nucleation centers and further biocomposite crystal growth is entirely governed by the pure electrostatic interactions between the protein’s surface and the positively charged Zn(II) metal ions. It was previously shown that enhancing these electrostatic interactions by a chemical modification of surface amino acid residues can lead to a rapid biocomposite crystal formation. However, a chemical modification of carbohydrate components by periodate oxidation for glycoproteins can serve as an alternative strategy. In the present study, an industrially important enzyme glucose oxidase (GOx) was selected as a model system. Periodate oxidation of GOx by 2.5 mM sodium periodate increased negative charge on the enzyme molecule, from −10.2 to −36.9 mV, as shown by zeta potential measurements and native PAGE electrophoresis. Biomineralization experiments with oxidized GOx resulted in higher specific activity, effectiveness factor, and higher thermostability of the ZIF-8 biocomposites. Periodate oxidation of carbohydrate components for glycoproteins can serve as a facile and general method for facilitating the biomimetic mineralization of other industrially relevant glycoproteins. |
| first_indexed | 2024-03-10T05:06:55Z |
| format | Article |
| id | doaj.art-52a1d6f1776d453aa8f977b0b9dae5b0 |
| institution | Directory Open Access Journal |
| issn | 2073-4360 |
| language | English |
| last_indexed | 2024-03-10T05:06:55Z |
| publishDate | 2021-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Polymers |
| spelling | doaj.art-52a1d6f1776d453aa8f977b0b9dae5b02023-11-23T01:07:57ZengMDPI AGPolymers2073-43602021-11-011322387510.3390/polym13223875Chemical Modification of Glycoproteins’ Carbohydrate Moiety as a General Strategy for the Synthesis of Efficient Biocatalysts by Biomimetic Mineralization: The Case of Glucose OxidaseMarija D. Stanišić0Nikolina Popović Kokar1Predrag Ristić2Ana Marija Balaž3Milan Senćanski4Miloš Ognjanović5Veljko R. Đokić6Radivoje Prodanović7Tamara R. Todorović8Faculty of Chemistry, University of Belgrade, Studentski trg 12-16, 11000 Belgrade, SerbiaFaculty of Chemistry, University of Belgrade, Studentski trg 12-16, 11000 Belgrade, SerbiaFaculty of Chemistry, University of Belgrade, Studentski trg 12-16, 11000 Belgrade, SerbiaInstitute of Chemistry, Technology and Metallurgy, National Institute of the Republic of Serbia, University of Belgrade, Njegoševa 6, 11000 Belgrade, SerbiaInstitute of Nuclear Sciences “Vinča”, National Institute of the Republic of Serbia, University of Belgrade, Mike Petrovića Alasa 12-14, 11000 Vinča-Belgrade, SerbiaInstitute of Nuclear Sciences “Vinča”, National Institute of the Republic of Serbia, University of Belgrade, Mike Petrovića Alasa 12-14, 11000 Vinča-Belgrade, SerbiaInnovation Center of the Faculty of Technology and Metallurgy, University of Belgrade, Karnegijeva 4, 11000 Belgrade, SerbiaFaculty of Chemistry, University of Belgrade, Studentski trg 12-16, 11000 Belgrade, SerbiaFaculty of Chemistry, University of Belgrade, Studentski trg 12-16, 11000 Belgrade, SerbiaZeolitic imidazolate framework-8 (ZIF-8) is widely used as a protective coating to encapsulate proteins via biomimetic mineralization. The formation of nucleation centers and further biocomposite crystal growth is entirely governed by the pure electrostatic interactions between the protein’s surface and the positively charged Zn(II) metal ions. It was previously shown that enhancing these electrostatic interactions by a chemical modification of surface amino acid residues can lead to a rapid biocomposite crystal formation. However, a chemical modification of carbohydrate components by periodate oxidation for glycoproteins can serve as an alternative strategy. In the present study, an industrially important enzyme glucose oxidase (GOx) was selected as a model system. Periodate oxidation of GOx by 2.5 mM sodium periodate increased negative charge on the enzyme molecule, from −10.2 to −36.9 mV, as shown by zeta potential measurements and native PAGE electrophoresis. Biomineralization experiments with oxidized GOx resulted in higher specific activity, effectiveness factor, and higher thermostability of the ZIF-8 biocomposites. Periodate oxidation of carbohydrate components for glycoproteins can serve as a facile and general method for facilitating the biomimetic mineralization of other industrially relevant glycoproteins.https://www.mdpi.com/2073-4360/13/22/3875metal–organic frameworksZIF-8biomimetic mineralizationbiocompositesbiocatalysts |
| spellingShingle | Marija D. Stanišić Nikolina Popović Kokar Predrag Ristić Ana Marija Balaž Milan Senćanski Miloš Ognjanović Veljko R. Đokić Radivoje Prodanović Tamara R. Todorović Chemical Modification of Glycoproteins’ Carbohydrate Moiety as a General Strategy for the Synthesis of Efficient Biocatalysts by Biomimetic Mineralization: The Case of Glucose Oxidase Polymers metal–organic frameworks ZIF-8 biomimetic mineralization biocomposites biocatalysts |
| title | Chemical Modification of Glycoproteins’ Carbohydrate Moiety as a General Strategy for the Synthesis of Efficient Biocatalysts by Biomimetic Mineralization: The Case of Glucose Oxidase |
| title_full | Chemical Modification of Glycoproteins’ Carbohydrate Moiety as a General Strategy for the Synthesis of Efficient Biocatalysts by Biomimetic Mineralization: The Case of Glucose Oxidase |
| title_fullStr | Chemical Modification of Glycoproteins’ Carbohydrate Moiety as a General Strategy for the Synthesis of Efficient Biocatalysts by Biomimetic Mineralization: The Case of Glucose Oxidase |
| title_full_unstemmed | Chemical Modification of Glycoproteins’ Carbohydrate Moiety as a General Strategy for the Synthesis of Efficient Biocatalysts by Biomimetic Mineralization: The Case of Glucose Oxidase |
| title_short | Chemical Modification of Glycoproteins’ Carbohydrate Moiety as a General Strategy for the Synthesis of Efficient Biocatalysts by Biomimetic Mineralization: The Case of Glucose Oxidase |
| title_sort | chemical modification of glycoproteins carbohydrate moiety as a general strategy for the synthesis of efficient biocatalysts by biomimetic mineralization the case of glucose oxidase |
| topic | metal–organic frameworks ZIF-8 biomimetic mineralization biocomposites biocatalysts |
| url | https://www.mdpi.com/2073-4360/13/22/3875 |
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