Characterization of biologically active exopolysaccharide produced by Streptomyces sp. NRCG4 and its anti-Alzheimer efficacy: in-vitro targets
Abstract Background Exopolysaccharides are extremely powerful molecules with a wide range of uses in pharmaceuticals due to their structural and compositional complexity. Marine microorganisms often produce bioactive substances with novel functions and structures because of their special living cond...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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SpringerOpen
2023-07-01
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| Series: | Journal of Genetic Engineering and Biotechnology |
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| Online Access: | https://doi.org/10.1186/s43141-023-00530-9 |
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| author | Manal G. Mahmoud Mohamed E. El Awady Manal S. Selim Abeer Y. Ibrahim Faten M. Ibrahim Sahar S. Mohamed |
| author_facet | Manal G. Mahmoud Mohamed E. El Awady Manal S. Selim Abeer Y. Ibrahim Faten M. Ibrahim Sahar S. Mohamed |
| author_sort | Manal G. Mahmoud |
| collection | DOAJ |
| description | Abstract Background Exopolysaccharides are extremely powerful molecules with a wide range of uses in pharmaceuticals due to their structural and compositional complexity. Marine microorganisms often produce bioactive substances with novel functions and structures because of their special living conditions. Polysaccharides from marine microorganisms are of interest to new drug discovery. Results The current research focused on the isolation of bacteria from Red Sea, Egypt, that have the ability to produce a new natural exopolysaccharide in order to be examined in treating Alzheimer’s illness to obviate side effects of synthetic drugs. Properties of exopolysaccharide (EPS) produced by an isolated Streptomyces strain were investigated for its capability to play as anti-Alzheimer. This strain was identified morphologically, physiologically, and biochemically and actually was confirmed by molecularly 16S rRNA analysis as Streptomyces sp. NRCG4 with accession number MK850242. The produced EPS was fractionated by precipitation 1:4 volumes of chilled ethanol and the third major fraction (1:3) listed as NRCG4, and then the functional groups, MW, and chemical evaluation have been detected via Fourier-transform infrared (FTIR), high-performance gel permeation chromatography (HPGPC), and high-performance liquid chromatography (HPLC). The findings showed that NRCG4 was an acidic EPS composed of mannuronic acid, glucose, mannose, and rhamnose in a molar ratio of 1.2:1.5:2.8:1.0, respectively. NRCG4 Mw was determined to be 4.25 × 105 gmol−1 and the Mn to be 1.97 × 105 gmol−1. Also, the NRCG4 included uronic acid (16.0%) and sulfate (0.0%), but no protein was found. In addition, antioxidant and anti-inflammation activity was measured through various methods. This study confirmed that NRCG4 exopolysaccharide exerted anti-Alzheimer’s characters via inhibition of cholinesterase and tyrosinase as well as anti-inflammatory and antioxidant abilities. Additionally, it occurred a potential role in the suppression of Alzheimer’s disease risk factors through its antioxidant (metal chelation, radical scavenging capability), anti-tyrosinase and anti-inflammatory characteristics. The anti-Alzheimer’s disease efficacy of NRCG4 exopolysaccharide may be assigned to its unique determined chemical composition. Conclusions The present study highlighted those exopolysaccharides could be harnessed to improve pharmaceutical industry (anti-Alzheimer, anti-tyrosinase, anti-inflammatory, and antioxidant agents). |
| first_indexed | 2024-03-13T00:40:32Z |
| format | Article |
| id | doaj.art-52a2eb0898144467b92ad42e8e23c56f |
| institution | Directory Open Access Journal |
| issn | 2090-5920 |
| language | English |
| last_indexed | 2024-03-13T00:40:32Z |
| publishDate | 2023-07-01 |
| publisher | SpringerOpen |
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| series | Journal of Genetic Engineering and Biotechnology |
| spelling | doaj.art-52a2eb0898144467b92ad42e8e23c56f2023-07-09T11:20:57ZengSpringerOpenJournal of Genetic Engineering and Biotechnology2090-59202023-07-0121111710.1186/s43141-023-00530-9Characterization of biologically active exopolysaccharide produced by Streptomyces sp. NRCG4 and its anti-Alzheimer efficacy: in-vitro targetsManal G. Mahmoud0Mohamed E. El Awady1Manal S. Selim2Abeer Y. Ibrahim3Faten M. Ibrahim4Sahar S. Mohamed5Microbial Biotechnology Department, National Research CentreMicrobial Biotechnology Department, National Research CentreMicrobial Biotechnology Department, National Research CentreMedicinal and Aromatic Plants Research Department, Pharmaceutical and Drug Industries Research Institute, National Research CentreMedicinal and Aromatic Plants Research Department, Pharmaceutical and Drug Industries Research Institute, National Research CentreMicrobial Biotechnology Department, National Research CentreAbstract Background Exopolysaccharides are extremely powerful molecules with a wide range of uses in pharmaceuticals due to their structural and compositional complexity. Marine microorganisms often produce bioactive substances with novel functions and structures because of their special living conditions. Polysaccharides from marine microorganisms are of interest to new drug discovery. Results The current research focused on the isolation of bacteria from Red Sea, Egypt, that have the ability to produce a new natural exopolysaccharide in order to be examined in treating Alzheimer’s illness to obviate side effects of synthetic drugs. Properties of exopolysaccharide (EPS) produced by an isolated Streptomyces strain were investigated for its capability to play as anti-Alzheimer. This strain was identified morphologically, physiologically, and biochemically and actually was confirmed by molecularly 16S rRNA analysis as Streptomyces sp. NRCG4 with accession number MK850242. The produced EPS was fractionated by precipitation 1:4 volumes of chilled ethanol and the third major fraction (1:3) listed as NRCG4, and then the functional groups, MW, and chemical evaluation have been detected via Fourier-transform infrared (FTIR), high-performance gel permeation chromatography (HPGPC), and high-performance liquid chromatography (HPLC). The findings showed that NRCG4 was an acidic EPS composed of mannuronic acid, glucose, mannose, and rhamnose in a molar ratio of 1.2:1.5:2.8:1.0, respectively. NRCG4 Mw was determined to be 4.25 × 105 gmol−1 and the Mn to be 1.97 × 105 gmol−1. Also, the NRCG4 included uronic acid (16.0%) and sulfate (0.0%), but no protein was found. In addition, antioxidant and anti-inflammation activity was measured through various methods. This study confirmed that NRCG4 exopolysaccharide exerted anti-Alzheimer’s characters via inhibition of cholinesterase and tyrosinase as well as anti-inflammatory and antioxidant abilities. Additionally, it occurred a potential role in the suppression of Alzheimer’s disease risk factors through its antioxidant (metal chelation, radical scavenging capability), anti-tyrosinase and anti-inflammatory characteristics. The anti-Alzheimer’s disease efficacy of NRCG4 exopolysaccharide may be assigned to its unique determined chemical composition. Conclusions The present study highlighted those exopolysaccharides could be harnessed to improve pharmaceutical industry (anti-Alzheimer, anti-tyrosinase, anti-inflammatory, and antioxidant agents).https://doi.org/10.1186/s43141-023-00530-9ExopolysaccharideStreptomycesAnti-AlzheimerAnti-tyrosinaseAnti-inflammatoryAntioxidant |
| spellingShingle | Manal G. Mahmoud Mohamed E. El Awady Manal S. Selim Abeer Y. Ibrahim Faten M. Ibrahim Sahar S. Mohamed Characterization of biologically active exopolysaccharide produced by Streptomyces sp. NRCG4 and its anti-Alzheimer efficacy: in-vitro targets Journal of Genetic Engineering and Biotechnology Exopolysaccharide Streptomyces Anti-Alzheimer Anti-tyrosinase Anti-inflammatory Antioxidant |
| title | Characterization of biologically active exopolysaccharide produced by Streptomyces sp. NRCG4 and its anti-Alzheimer efficacy: in-vitro targets |
| title_full | Characterization of biologically active exopolysaccharide produced by Streptomyces sp. NRCG4 and its anti-Alzheimer efficacy: in-vitro targets |
| title_fullStr | Characterization of biologically active exopolysaccharide produced by Streptomyces sp. NRCG4 and its anti-Alzheimer efficacy: in-vitro targets |
| title_full_unstemmed | Characterization of biologically active exopolysaccharide produced by Streptomyces sp. NRCG4 and its anti-Alzheimer efficacy: in-vitro targets |
| title_short | Characterization of biologically active exopolysaccharide produced by Streptomyces sp. NRCG4 and its anti-Alzheimer efficacy: in-vitro targets |
| title_sort | characterization of biologically active exopolysaccharide produced by streptomyces sp nrcg4 and its anti alzheimer efficacy in vitro targets |
| topic | Exopolysaccharide Streptomyces Anti-Alzheimer Anti-tyrosinase Anti-inflammatory Antioxidant |
| url | https://doi.org/10.1186/s43141-023-00530-9 |
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