<i>Viburnum opulus</i> Fruit Phenolic Compounds as Cytoprotective Agents Able to Decrease Free Fatty Acids and Glucose Uptake by Caco-2 Cells
In recent years, there has been increasing interest in studying food-originated phytocompounds with beneficial influences for humans. Amongst the most active natural substances are polyphenols, for which high content has been identified in the <i>Viburnum opulus</i> berry, and which are...
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| Format: | Article |
| Language: | English |
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MDPI AG
2019-08-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/8/8/262 |
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| author | Małgorzata Zakłos-Szyda Nina Pawlik Dominika Polka Adriana Nowak Maria Koziołkiewicz Anna Podsędek |
| author_facet | Małgorzata Zakłos-Szyda Nina Pawlik Dominika Polka Adriana Nowak Maria Koziołkiewicz Anna Podsędek |
| author_sort | Małgorzata Zakłos-Szyda |
| collection | DOAJ |
| description | In recent years, there has been increasing interest in studying food-originated phytocompounds with beneficial influences for humans. Amongst the most active natural substances are polyphenols, for which high content has been identified in the <i>Viburnum opulus</i> berry, and which are unused in Western Europe. Due to its strong antioxidant activity we explored the potential of <i>V. opulus</i> as a preventive agent against diet-related chronic diseases, such as obesity and type 2 diabetes. Among the causes of these ailments is oxidative stress, as well as impaired glucose and free fatty acids (FFA) uptake. Thus, the purpose of this study was to determine biological activity of <i>V. opulus</i> phenolic extracts as cytoprotective agents able to decrease induced oxidative stress, lower lipid accumulation and attenuate glucose and FFA uptake by Caco-2 cells via GLUT2 and CD36/FAT transporters. To determine the source of the most biologically active phenolic compounds, we obtained four phenolic compounds extracts as crude juice, phenolics isolated from juice and two preparations of phenolics obtained with different extraction agents from fruit pomace. Among the studied extracts, the phenolic rich fraction obtained from fruit juice revealed the strongest activity to decrease uptake of glucose, FFA and accumulation of lipid droplets in Caco-2 cells without affecting their viability (IC<sub>0</sub> 50 μg/mL). Observed uptake attenuation was followed by decrease of the <i>CD36/FAT</i> gene expression, without influence on the <i>GLUT2</i> and <i>PPARα</i> levels. We suspect that <i>V. opulus</i> phenolics were able to modulate the cellular membrane dynamic, although that hypothesis requires further, more detailed studies. Extracts revealed strong chemo-preventive activity against oxidative stress induced chemically by tert-butylhydroperoxide (t-BOOH), as well as against DNA damage through the induction of DNA repair after cell exposition to methylnitronitrosoguanidine (MNNG) and H<sub>2</sub>O<sub>2</sub>. Our findings suggest <i>Viburnum opulus</i> fruit as a dietary source of phytocompounds, which could be considered as a tailored design food supplement components for the prevention and treatment of postprandial elevation of glucose and fatty acids through delaying the rate of glucose and fatty acid absorption by intestinal cells. |
| first_indexed | 2024-03-12T08:02:53Z |
| format | Article |
| id | doaj.art-52a40951dbc34eaeadd05f026005c12e |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-12T08:02:53Z |
| publishDate | 2019-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-52a40951dbc34eaeadd05f026005c12e2023-09-02T19:46:07ZengMDPI AGAntioxidants2076-39212019-08-018826210.3390/antiox8080262antiox8080262<i>Viburnum opulus</i> Fruit Phenolic Compounds as Cytoprotective Agents Able to Decrease Free Fatty Acids and Glucose Uptake by Caco-2 CellsMałgorzata Zakłos-Szyda0Nina Pawlik1Dominika Polka2Adriana Nowak3Maria Koziołkiewicz4Anna Podsędek5Institute of Technical Biochemistry, Faculty of Biotechnology and Food Sciences, Lodz University of Technology, Stefanowskiego 4/10, 90-924 Lodz, PolandInstitute of Technical Biochemistry, Faculty of Biotechnology and Food Sciences, Lodz University of Technology, Stefanowskiego 4/10, 90-924 Lodz, PolandInstitute of Technical Biochemistry, Faculty of Biotechnology and Food Sciences, Lodz University of Technology, Stefanowskiego 4/10, 90-924 Lodz, PolandInstitute of Fermentation Technology and Microbiology, Faculty of Biotechnology and Food Sciences, Lodz University of Technology, Wólczańska 171/173, 90-924 Lodz, PolandInstitute of Technical Biochemistry, Faculty of Biotechnology and Food Sciences, Lodz University of Technology, Stefanowskiego 4/10, 90-924 Lodz, PolandInstitute of Technical Biochemistry, Faculty of Biotechnology and Food Sciences, Lodz University of Technology, Stefanowskiego 4/10, 90-924 Lodz, PolandIn recent years, there has been increasing interest in studying food-originated phytocompounds with beneficial influences for humans. Amongst the most active natural substances are polyphenols, for which high content has been identified in the <i>Viburnum opulus</i> berry, and which are unused in Western Europe. Due to its strong antioxidant activity we explored the potential of <i>V. opulus</i> as a preventive agent against diet-related chronic diseases, such as obesity and type 2 diabetes. Among the causes of these ailments is oxidative stress, as well as impaired glucose and free fatty acids (FFA) uptake. Thus, the purpose of this study was to determine biological activity of <i>V. opulus</i> phenolic extracts as cytoprotective agents able to decrease induced oxidative stress, lower lipid accumulation and attenuate glucose and FFA uptake by Caco-2 cells via GLUT2 and CD36/FAT transporters. To determine the source of the most biologically active phenolic compounds, we obtained four phenolic compounds extracts as crude juice, phenolics isolated from juice and two preparations of phenolics obtained with different extraction agents from fruit pomace. Among the studied extracts, the phenolic rich fraction obtained from fruit juice revealed the strongest activity to decrease uptake of glucose, FFA and accumulation of lipid droplets in Caco-2 cells without affecting their viability (IC<sub>0</sub> 50 μg/mL). Observed uptake attenuation was followed by decrease of the <i>CD36/FAT</i> gene expression, without influence on the <i>GLUT2</i> and <i>PPARα</i> levels. We suspect that <i>V. opulus</i> phenolics were able to modulate the cellular membrane dynamic, although that hypothesis requires further, more detailed studies. Extracts revealed strong chemo-preventive activity against oxidative stress induced chemically by tert-butylhydroperoxide (t-BOOH), as well as against DNA damage through the induction of DNA repair after cell exposition to methylnitronitrosoguanidine (MNNG) and H<sub>2</sub>O<sub>2</sub>. Our findings suggest <i>Viburnum opulus</i> fruit as a dietary source of phytocompounds, which could be considered as a tailored design food supplement components for the prevention and treatment of postprandial elevation of glucose and fatty acids through delaying the rate of glucose and fatty acid absorption by intestinal cells.https://www.mdpi.com/2076-3921/8/8/262<i>Viburnum opulus</i>phenolic compoundsfree fatty acids uptakeglucose uptakecytoprotection |
| spellingShingle | Małgorzata Zakłos-Szyda Nina Pawlik Dominika Polka Adriana Nowak Maria Koziołkiewicz Anna Podsędek <i>Viburnum opulus</i> Fruit Phenolic Compounds as Cytoprotective Agents Able to Decrease Free Fatty Acids and Glucose Uptake by Caco-2 Cells Antioxidants <i>Viburnum opulus</i> phenolic compounds free fatty acids uptake glucose uptake cytoprotection |
| title | <i>Viburnum opulus</i> Fruit Phenolic Compounds as Cytoprotective Agents Able to Decrease Free Fatty Acids and Glucose Uptake by Caco-2 Cells |
| title_full | <i>Viburnum opulus</i> Fruit Phenolic Compounds as Cytoprotective Agents Able to Decrease Free Fatty Acids and Glucose Uptake by Caco-2 Cells |
| title_fullStr | <i>Viburnum opulus</i> Fruit Phenolic Compounds as Cytoprotective Agents Able to Decrease Free Fatty Acids and Glucose Uptake by Caco-2 Cells |
| title_full_unstemmed | <i>Viburnum opulus</i> Fruit Phenolic Compounds as Cytoprotective Agents Able to Decrease Free Fatty Acids and Glucose Uptake by Caco-2 Cells |
| title_short | <i>Viburnum opulus</i> Fruit Phenolic Compounds as Cytoprotective Agents Able to Decrease Free Fatty Acids and Glucose Uptake by Caco-2 Cells |
| title_sort | i viburnum opulus i fruit phenolic compounds as cytoprotective agents able to decrease free fatty acids and glucose uptake by caco 2 cells |
| topic | <i>Viburnum opulus</i> phenolic compounds free fatty acids uptake glucose uptake cytoprotection |
| url | https://www.mdpi.com/2076-3921/8/8/262 |
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