Transcriptomics and Targeted Proteomics Analysis to Gain Insights Into the Immune-control Mechanisms of HIV-1 Infected Elite Controllers
A small subset of HIV-1 infected individuals, the “Elite Controllers” (EC), can control viral replication and restrain progression to immunodeficiency without antiretroviral therapy (ART). In this study, a cross-sectional transcriptomics and targeted proteomics analysis were performed in a well-defi...
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| Format: | Article |
| Language: | English |
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Elsevier
2018-01-01
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| Series: | EBioMedicine |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352396417304735 |
| _version_ | 1819172276168294400 |
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| author | Wang Zhang Anoop T. Ambikan Maike Sperk Robert van Domselaar Piotr Nowak Kajsa Noyan Aman Russom Anders Sönnerborg Ujjwal Neogi |
| author_facet | Wang Zhang Anoop T. Ambikan Maike Sperk Robert van Domselaar Piotr Nowak Kajsa Noyan Aman Russom Anders Sönnerborg Ujjwal Neogi |
| author_sort | Wang Zhang |
| collection | DOAJ |
| description | A small subset of HIV-1 infected individuals, the “Elite Controllers” (EC), can control viral replication and restrain progression to immunodeficiency without antiretroviral therapy (ART). In this study, a cross-sectional transcriptomics and targeted proteomics analysis were performed in a well-defined Swedish cohort of untreated EC (n = 19), treatment naïve patients with viremia (VP, n = 32) and HIV-1-negative healthy controls (HC, n = 23). The blood transcriptome identified 151 protein-coding genes that were differentially expressed (DE) in VP compared to EC. Genes like CXCR6 and SIGLEC1 were downregulated in EC compared to VP. A definite distinction in gene expression between males and females among all patient-groups were observed. The gene expression profile between female EC and the healthy females was similar but did differ between male EC and healthy males. At targeted proteomics analysis, 90% (29/32) of VPs clustered together while EC and HC clustered separately from VP. Among the soluble factors, 33 were distinctive to be statistically significant (False discovery rate = 0.02). Cell surface receptor signaling pathway, programmed cell death, response to cytokine and cytokine-mediated signaling seem to synergistically play an essential role in HIV-1 control in EC. |
| first_indexed | 2024-12-22T20:04:36Z |
| format | Article |
| id | doaj.art-52a544ced231469bb300f542df5dc161 |
| institution | Directory Open Access Journal |
| issn | 2352-3964 |
| language | English |
| last_indexed | 2024-12-22T20:04:36Z |
| publishDate | 2018-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | EBioMedicine |
| spelling | doaj.art-52a544ced231469bb300f542df5dc1612022-12-21T18:14:10ZengElsevierEBioMedicine2352-39642018-01-0127C405010.1016/j.ebiom.2017.11.031Transcriptomics and Targeted Proteomics Analysis to Gain Insights Into the Immune-control Mechanisms of HIV-1 Infected Elite ControllersWang Zhang0Anoop T. Ambikan1Maike Sperk2Robert van Domselaar3Piotr Nowak4Kajsa Noyan5Aman Russom6Anders Sönnerborg7Ujjwal Neogi8Division of Clinical Microbiology, Department of Laboratory Medicine, Karolinska Institutet, Stockholm, SwedenDivision of Clinical Microbiology, Department of Laboratory Medicine, Karolinska Institutet, Stockholm, SwedenDivision of Clinical Microbiology, Department of Laboratory Medicine, Karolinska Institutet, Stockholm, SwedenDepartment of Medicine Huddinge, Unit of Infectious Diseases, Karolinska Institutet, Karolinska University Hospital, Stockholm, SwedenDivision of Clinical Microbiology, Department of Laboratory Medicine, Karolinska Institutet, Stockholm, SwedenDivision of Clinical Microbiology, Department of Laboratory Medicine, Karolinska Institutet, Stockholm, SwedenScience for Life Laboratory, Division of Proteomics and Nanobiotechnology, KTH Royal Institute of Technology, Solna, Stockholm, SwedenDivision of Clinical Microbiology, Department of Laboratory Medicine, Karolinska Institutet, Stockholm, SwedenDivision of Clinical Microbiology, Department of Laboratory Medicine, Karolinska Institutet, Stockholm, SwedenA small subset of HIV-1 infected individuals, the “Elite Controllers” (EC), can control viral replication and restrain progression to immunodeficiency without antiretroviral therapy (ART). In this study, a cross-sectional transcriptomics and targeted proteomics analysis were performed in a well-defined Swedish cohort of untreated EC (n = 19), treatment naïve patients with viremia (VP, n = 32) and HIV-1-negative healthy controls (HC, n = 23). The blood transcriptome identified 151 protein-coding genes that were differentially expressed (DE) in VP compared to EC. Genes like CXCR6 and SIGLEC1 were downregulated in EC compared to VP. A definite distinction in gene expression between males and females among all patient-groups were observed. The gene expression profile between female EC and the healthy females was similar but did differ between male EC and healthy males. At targeted proteomics analysis, 90% (29/32) of VPs clustered together while EC and HC clustered separately from VP. Among the soluble factors, 33 were distinctive to be statistically significant (False discovery rate = 0.02). Cell surface receptor signaling pathway, programmed cell death, response to cytokine and cytokine-mediated signaling seem to synergistically play an essential role in HIV-1 control in EC.http://www.sciencedirect.com/science/article/pii/S2352396417304735HIV-1 Elite ControllersTranscriptomeProteome |
| spellingShingle | Wang Zhang Anoop T. Ambikan Maike Sperk Robert van Domselaar Piotr Nowak Kajsa Noyan Aman Russom Anders Sönnerborg Ujjwal Neogi Transcriptomics and Targeted Proteomics Analysis to Gain Insights Into the Immune-control Mechanisms of HIV-1 Infected Elite Controllers EBioMedicine HIV-1 Elite Controllers Transcriptome Proteome |
| title | Transcriptomics and Targeted Proteomics Analysis to Gain Insights Into the Immune-control Mechanisms of HIV-1 Infected Elite Controllers |
| title_full | Transcriptomics and Targeted Proteomics Analysis to Gain Insights Into the Immune-control Mechanisms of HIV-1 Infected Elite Controllers |
| title_fullStr | Transcriptomics and Targeted Proteomics Analysis to Gain Insights Into the Immune-control Mechanisms of HIV-1 Infected Elite Controllers |
| title_full_unstemmed | Transcriptomics and Targeted Proteomics Analysis to Gain Insights Into the Immune-control Mechanisms of HIV-1 Infected Elite Controllers |
| title_short | Transcriptomics and Targeted Proteomics Analysis to Gain Insights Into the Immune-control Mechanisms of HIV-1 Infected Elite Controllers |
| title_sort | transcriptomics and targeted proteomics analysis to gain insights into the immune control mechanisms of hiv 1 infected elite controllers |
| topic | HIV-1 Elite Controllers Transcriptome Proteome |
| url | http://www.sciencedirect.com/science/article/pii/S2352396417304735 |
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