Encapsulation of Benzyl Isothiocyanate with β-Cyclodextrin Using Ultrasonication: Preparation, Characterization, and Antibacterial Assay
Benzyl isothiocyanate (BITC) is widely utilized in multiple biomedical fields, due to its significant antibacterial properties and low toxicity. However, poor water solubility and pungent odor has limited its application in the food industry. In this study, we first prepared inclusion complexes of B...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-11-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/11/22/3724 |
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| author | Hongbo Li Xujia Ming Zhen Wang Jiaqi Li Yunxia Liang Dan Xu Zhenbin Liu Liangbin Hu Haizhen Mo |
| author_facet | Hongbo Li Xujia Ming Zhen Wang Jiaqi Li Yunxia Liang Dan Xu Zhenbin Liu Liangbin Hu Haizhen Mo |
| author_sort | Hongbo Li |
| collection | DOAJ |
| description | Benzyl isothiocyanate (BITC) is widely utilized in multiple biomedical fields, due to its significant antibacterial properties and low toxicity. However, poor water solubility and pungent odor has limited its application in the food industry. In this study, we first prepared inclusion complexes of BITC in GLU-β-CD and HP-β-CD using ultrasound, which is able to overcome the hindrance of poor water solubility and high volatility. Then, the BITC-β-CD inclusion complexes were characterized by using high-performance liquid chromatography (HPLC), nuclear magnetic resonance hydrogen spectra (<sup>1</sup>H-NMR), infrared absorption spectra (IR), and differential scanning calorimetry (DSC) to confirm their stability. Further, the evaluation of antibacterial and antitumor effects of the BITC-β-CD inclusion complexes showed that they had great bactericidal activity against both <i>Escherichia coli</i> and <i>Staphylococcus aureus</i> cells, and also inhibited the growth of HepG2 cells in vitro. In addition, our results indicated that BITC-β-CD complexes were able to inhibit the growth of <i>S. aureus</i> in broccoli juice and extend the shelf life of broccoli juice, demonstrating the potential of β-cyclodextrin to improve the stability and controlled release of BITC. Taken together, our results show that BITC-β-CD complexes have good potential for application in the food industry. |
| first_indexed | 2024-03-09T18:19:32Z |
| format | Article |
| id | doaj.art-52a632dd08f24c6bb90b92196bd33994 |
| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-03-09T18:19:32Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj.art-52a632dd08f24c6bb90b92196bd339942023-11-24T08:23:17ZengMDPI AGFoods2304-81582022-11-011122372410.3390/foods11223724Encapsulation of Benzyl Isothiocyanate with β-Cyclodextrin Using Ultrasonication: Preparation, Characterization, and Antibacterial AssayHongbo Li0Xujia Ming1Zhen Wang2Jiaqi Li3Yunxia Liang4Dan Xu5Zhenbin Liu6Liangbin Hu7Haizhen Mo8School of Food Science and Engineering, Shaanxi University of Science and Technology, Xi’an 710021, ChinaSchool of Food Science and Engineering, Shaanxi University of Science and Technology, Xi’an 710021, ChinaSchool of Food Science and Engineering, Shaanxi University of Science and Technology, Xi’an 710021, ChinaDepartment of Food Science, Henan Institute of Science and Technology, Xinxiang 453003, ChinaSchool of Food Science and Engineering, Shaanxi University of Science and Technology, Xi’an 710021, ChinaSchool of Food Science and Engineering, Shaanxi University of Science and Technology, Xi’an 710021, ChinaSchool of Food Science and Engineering, Shaanxi University of Science and Technology, Xi’an 710021, ChinaSchool of Food Science and Engineering, Shaanxi University of Science and Technology, Xi’an 710021, ChinaSchool of Food Science and Engineering, Shaanxi University of Science and Technology, Xi’an 710021, ChinaBenzyl isothiocyanate (BITC) is widely utilized in multiple biomedical fields, due to its significant antibacterial properties and low toxicity. However, poor water solubility and pungent odor has limited its application in the food industry. In this study, we first prepared inclusion complexes of BITC in GLU-β-CD and HP-β-CD using ultrasound, which is able to overcome the hindrance of poor water solubility and high volatility. Then, the BITC-β-CD inclusion complexes were characterized by using high-performance liquid chromatography (HPLC), nuclear magnetic resonance hydrogen spectra (<sup>1</sup>H-NMR), infrared absorption spectra (IR), and differential scanning calorimetry (DSC) to confirm their stability. Further, the evaluation of antibacterial and antitumor effects of the BITC-β-CD inclusion complexes showed that they had great bactericidal activity against both <i>Escherichia coli</i> and <i>Staphylococcus aureus</i> cells, and also inhibited the growth of HepG2 cells in vitro. In addition, our results indicated that BITC-β-CD complexes were able to inhibit the growth of <i>S. aureus</i> in broccoli juice and extend the shelf life of broccoli juice, demonstrating the potential of β-cyclodextrin to improve the stability and controlled release of BITC. Taken together, our results show that BITC-β-CD complexes have good potential for application in the food industry.https://www.mdpi.com/2304-8158/11/22/3724benzyl isothiocyanateBITC-β-CD complexantimicrobial activitybroccoli juice |
| spellingShingle | Hongbo Li Xujia Ming Zhen Wang Jiaqi Li Yunxia Liang Dan Xu Zhenbin Liu Liangbin Hu Haizhen Mo Encapsulation of Benzyl Isothiocyanate with β-Cyclodextrin Using Ultrasonication: Preparation, Characterization, and Antibacterial Assay Foods benzyl isothiocyanate BITC-β-CD complex antimicrobial activity broccoli juice |
| title | Encapsulation of Benzyl Isothiocyanate with β-Cyclodextrin Using Ultrasonication: Preparation, Characterization, and Antibacterial Assay |
| title_full | Encapsulation of Benzyl Isothiocyanate with β-Cyclodextrin Using Ultrasonication: Preparation, Characterization, and Antibacterial Assay |
| title_fullStr | Encapsulation of Benzyl Isothiocyanate with β-Cyclodextrin Using Ultrasonication: Preparation, Characterization, and Antibacterial Assay |
| title_full_unstemmed | Encapsulation of Benzyl Isothiocyanate with β-Cyclodextrin Using Ultrasonication: Preparation, Characterization, and Antibacterial Assay |
| title_short | Encapsulation of Benzyl Isothiocyanate with β-Cyclodextrin Using Ultrasonication: Preparation, Characterization, and Antibacterial Assay |
| title_sort | encapsulation of benzyl isothiocyanate with β cyclodextrin using ultrasonication preparation characterization and antibacterial assay |
| topic | benzyl isothiocyanate BITC-β-CD complex antimicrobial activity broccoli juice |
| url | https://www.mdpi.com/2304-8158/11/22/3724 |
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