Homogeneous Deformation of Native Erythrocytes During Long-Term Storage
Purpose of the study — to evaluate biomechanical regularities of deep deformation of native erythrocytes’ membranes during long-term (up to 32 days) storage of erythrocyte suspension.Materials and methods. The method for addressing the said problem was atomic-force spectroscopy. The measured value w...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Federal Research and Clinical Center of Intensive Care Medicine and Rehabilitology, Moscow, Russia
2019-11-01
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| Series: | Общая реаниматология |
| Subjects: | |
| Online Access: | https://www.reanimatology.com/rmt/article/view/1811 |
| Summary: | Purpose of the study — to evaluate biomechanical regularities of deep deformation of native erythrocytes’ membranes during long-term (up to 32 days) storage of erythrocyte suspension.Materials and methods. The method for addressing the said problem was atomic-force spectroscopy. The measured value was hHz comprizing the depth to which the probe immersion process was described by interaction with a homogeneous medium. Empirical and theoretical dependence of the interaction force F (nN) on the probe immersion depth h (nm) — F (h) were obtained. Bar charts of relative frequency density of Young’s modulus E were built.Results. Modulus E changed from 9.3±3.2 kPa — for 3 days of storage, to 22.7±8.7 kPa — for 32 days. Coefficients of skewness were 0.52±0.04 (for day 3) and 0.82±0.09 (for day 32 d), hHz value remaining constant.Conclusion. Progressively as erythrocyte suspension was stored, erythrocyte membranes to the depth of 700 nm deflected homogeneously in spite of 2.4-fold increase of Young’s modulus. |
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| ISSN: | 1813-9779 2411-7110 |