Homogeneous Deformation of Native Erythrocytes During Long-Term Storage
Purpose of the study — to evaluate biomechanical regularities of deep deformation of native erythrocytes’ membranes during long-term (up to 32 days) storage of erythrocyte suspension.Materials and methods. The method for addressing the said problem was atomic-force spectroscopy. The measured value w...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Federal Research and Clinical Center of Intensive Care Medicine and Rehabilitology, Moscow, Russia
2019-11-01
|
| Series: | Общая реаниматология |
| Subjects: | |
| Online Access: | https://www.reanimatology.com/rmt/article/view/1811 |
| _version_ | 1826562164293369856 |
|---|---|
| author | E. A. Manchenko E. K. Kozlova V. A. Sergunova A. M. Chernysh |
| author_facet | E. A. Manchenko E. K. Kozlova V. A. Sergunova A. M. Chernysh |
| author_sort | E. A. Manchenko |
| collection | DOAJ |
| description | Purpose of the study — to evaluate biomechanical regularities of deep deformation of native erythrocytes’ membranes during long-term (up to 32 days) storage of erythrocyte suspension.Materials and methods. The method for addressing the said problem was atomic-force spectroscopy. The measured value was hHz comprizing the depth to which the probe immersion process was described by interaction with a homogeneous medium. Empirical and theoretical dependence of the interaction force F (nN) on the probe immersion depth h (nm) — F (h) were obtained. Bar charts of relative frequency density of Young’s modulus E were built.Results. Modulus E changed from 9.3±3.2 kPa — for 3 days of storage, to 22.7±8.7 kPa — for 32 days. Coefficients of skewness were 0.52±0.04 (for day 3) and 0.82±0.09 (for day 32 d), hHz value remaining constant.Conclusion. Progressively as erythrocyte suspension was stored, erythrocyte membranes to the depth of 700 nm deflected homogeneously in spite of 2.4-fold increase of Young’s modulus. |
| first_indexed | 2024-04-10T01:27:04Z |
| format | Article |
| id | doaj.art-52a731b6a5c7404bb95f55fdf06da310 |
| institution | Directory Open Access Journal |
| issn | 1813-9779 2411-7110 |
| language | English |
| last_indexed | 2025-03-14T09:43:43Z |
| publishDate | 2019-11-01 |
| publisher | Federal Research and Clinical Center of Intensive Care Medicine and Rehabilitology, Moscow, Russia |
| record_format | Article |
| series | Общая реаниматология |
| spelling | doaj.art-52a731b6a5c7404bb95f55fdf06da3102025-03-02T11:29:37ZengFederal Research and Clinical Center of Intensive Care Medicine and Rehabilitology, Moscow, RussiaОбщая реаниматология1813-97792411-71102019-11-0115541010.15360/1813-9779-2019-5-4-101702Homogeneous Deformation of Native Erythrocytes During Long-Term StorageE. A. Manchenko0E. K. Kozlova1V. A. Sergunova2A. M. Chernysh3V. A. Negovsky Research Institute of General Reanimatology, Federal Research and Clinical Center of Intensive Care Medicine and Rehabilitology, I. M. Sechenov First Moscow State Medical University, Ministry of Health of RussiaV. A. Negovsky Research Institute of General Reanimatology, Federal Research and Clinical Center of Intensive Care Medicine and Rehabilitology, I. M. Sechenov First Moscow State Medical University, Ministry of Health of RussiaV. A. Negovsky Research Institute of General Reanimatology, Federal Research and Clinical Center of Intensive Care Medicine and RehabilitologyV. A. Negovsky Research Institute of General Reanimatology, Federal Research and Clinical Center of Intensive Care Medicine and Rehabilitology, I. M. Sechenov First Moscow State Medical University, Ministry of Health of RussiaPurpose of the study — to evaluate biomechanical regularities of deep deformation of native erythrocytes’ membranes during long-term (up to 32 days) storage of erythrocyte suspension.Materials and methods. The method for addressing the said problem was atomic-force spectroscopy. The measured value was hHz comprizing the depth to which the probe immersion process was described by interaction with a homogeneous medium. Empirical and theoretical dependence of the interaction force F (nN) on the probe immersion depth h (nm) — F (h) were obtained. Bar charts of relative frequency density of Young’s modulus E were built.Results. Modulus E changed from 9.3±3.2 kPa — for 3 days of storage, to 22.7±8.7 kPa — for 32 days. Coefficients of skewness were 0.52±0.04 (for day 3) and 0.82±0.09 (for day 32 d), hHz value remaining constant.Conclusion. Progressively as erythrocyte suspension was stored, erythrocyte membranes to the depth of 700 nm deflected homogeneously in spite of 2.4-fold increase of Young’s modulus.https://www.reanimatology.com/rmt/article/view/1811erythrocytesmembrane stiffnessdeformationblood storageatomic-force spectroscopy |
| spellingShingle | E. A. Manchenko E. K. Kozlova V. A. Sergunova A. M. Chernysh Homogeneous Deformation of Native Erythrocytes During Long-Term Storage Общая реаниматология erythrocytes membrane stiffness deformation blood storage atomic-force spectroscopy |
| title | Homogeneous Deformation of Native Erythrocytes During Long-Term Storage |
| title_full | Homogeneous Deformation of Native Erythrocytes During Long-Term Storage |
| title_fullStr | Homogeneous Deformation of Native Erythrocytes During Long-Term Storage |
| title_full_unstemmed | Homogeneous Deformation of Native Erythrocytes During Long-Term Storage |
| title_short | Homogeneous Deformation of Native Erythrocytes During Long-Term Storage |
| title_sort | homogeneous deformation of native erythrocytes during long term storage |
| topic | erythrocytes membrane stiffness deformation blood storage atomic-force spectroscopy |
| url | https://www.reanimatology.com/rmt/article/view/1811 |
| work_keys_str_mv | AT eamanchenko homogeneousdeformationofnativeerythrocytesduringlongtermstorage AT ekkozlova homogeneousdeformationofnativeerythrocytesduringlongtermstorage AT vasergunova homogeneousdeformationofnativeerythrocytesduringlongtermstorage AT amchernysh homogeneousdeformationofnativeerythrocytesduringlongtermstorage |