Homogeneous Deformation of Native Erythrocytes During Long-Term Storage
Purpose of the study — to evaluate biomechanical regularities of deep deformation of native erythrocytes’ membranes during long-term (up to 32 days) storage of erythrocyte suspension.Materials and methods. The method for addressing the said problem was atomic-force spectroscopy. The measured value w...
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| Format: | Article |
| Language: | English |
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Federal Research and Clinical Center of Intensive Care Medicine and Rehabilitology, Moscow, Russia
2019-11-01
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| Series: | Общая реаниматология |
| Subjects: | |
| Online Access: | https://www.reanimatology.com/rmt/article/view/1811 |
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| author | E. A. Manchenko E. K. Kozlova V. A. Sergunova A. M. Chernysh |
| author_facet | E. A. Manchenko E. K. Kozlova V. A. Sergunova A. M. Chernysh |
| author_sort | E. A. Manchenko |
| collection | DOAJ |
| description | Purpose of the study — to evaluate biomechanical regularities of deep deformation of native erythrocytes’ membranes during long-term (up to 32 days) storage of erythrocyte suspension.Materials and methods. The method for addressing the said problem was atomic-force spectroscopy. The measured value was hHz comprizing the depth to which the probe immersion process was described by interaction with a homogeneous medium. Empirical and theoretical dependence of the interaction force F (nN) on the probe immersion depth h (nm) — F (h) were obtained. Bar charts of relative frequency density of Young’s modulus E were built.Results. Modulus E changed from 9.3±3.2 kPa — for 3 days of storage, to 22.7±8.7 kPa — for 32 days. Coefficients of skewness were 0.52±0.04 (for day 3) and 0.82±0.09 (for day 32 d), hHz value remaining constant.Conclusion. Progressively as erythrocyte suspension was stored, erythrocyte membranes to the depth of 700 nm deflected homogeneously in spite of 2.4-fold increase of Young’s modulus. |
| first_indexed | 2024-04-10T01:27:04Z |
| format | Article |
| id | doaj.art-52a731b6a5c7404bb95f55fdf06da310 |
| institution | Directory Open Access Journal |
| issn | 1813-9779 2411-7110 |
| language | English |
| last_indexed | 2024-04-10T01:27:04Z |
| publishDate | 2019-11-01 |
| publisher | Federal Research and Clinical Center of Intensive Care Medicine and Rehabilitology, Moscow, Russia |
| record_format | Article |
| series | Общая реаниматология |
| spelling | doaj.art-52a731b6a5c7404bb95f55fdf06da3102023-03-13T09:32:56ZengFederal Research and Clinical Center of Intensive Care Medicine and Rehabilitology, Moscow, RussiaОбщая реаниматология1813-97792411-71102019-11-0115541010.15360/1813-9779-2019-5-4-101702Homogeneous Deformation of Native Erythrocytes During Long-Term StorageE. A. Manchenko0E. K. Kozlova1V. A. Sergunova2A. M. Chernysh3НИИ общей реаниматологии им. В. А. Неговского ФНКЦ РР, Первый Московский государственный медицинский университет им. И. М. Сеченова Минздрава РоссииНИИ общей реаниматологии им. В. А. Неговского ФНКЦ РР, Первый Московский государственный медицинский университет им. И. М. Сеченова Минздрава РоссииНИИ общей реаниматологии им. В. А. Неговского ФНКЦ РРНИИ общей реаниматологии им. В. А. Неговского ФНКЦ РР, Первый Московский государственный медицинский университет им. И. М. Сеченова Минздрава РоссииPurpose of the study — to evaluate biomechanical regularities of deep deformation of native erythrocytes’ membranes during long-term (up to 32 days) storage of erythrocyte suspension.Materials and methods. The method for addressing the said problem was atomic-force spectroscopy. The measured value was hHz comprizing the depth to which the probe immersion process was described by interaction with a homogeneous medium. Empirical and theoretical dependence of the interaction force F (nN) on the probe immersion depth h (nm) — F (h) were obtained. Bar charts of relative frequency density of Young’s modulus E were built.Results. Modulus E changed from 9.3±3.2 kPa — for 3 days of storage, to 22.7±8.7 kPa — for 32 days. Coefficients of skewness were 0.52±0.04 (for day 3) and 0.82±0.09 (for day 32 d), hHz value remaining constant.Conclusion. Progressively as erythrocyte suspension was stored, erythrocyte membranes to the depth of 700 nm deflected homogeneously in spite of 2.4-fold increase of Young’s modulus.https://www.reanimatology.com/rmt/article/view/1811эритроцитыжесткость мембраныдеформацияхранение кровиатомно-силовая спектроскопия |
| spellingShingle | E. A. Manchenko E. K. Kozlova V. A. Sergunova A. M. Chernysh Homogeneous Deformation of Native Erythrocytes During Long-Term Storage Общая реаниматология эритроциты жесткость мембраны деформация хранение крови атомно-силовая спектроскопия |
| title | Homogeneous Deformation of Native Erythrocytes During Long-Term Storage |
| title_full | Homogeneous Deformation of Native Erythrocytes During Long-Term Storage |
| title_fullStr | Homogeneous Deformation of Native Erythrocytes During Long-Term Storage |
| title_full_unstemmed | Homogeneous Deformation of Native Erythrocytes During Long-Term Storage |
| title_short | Homogeneous Deformation of Native Erythrocytes During Long-Term Storage |
| title_sort | homogeneous deformation of native erythrocytes during long term storage |
| topic | эритроциты жесткость мембраны деформация хранение крови атомно-силовая спектроскопия |
| url | https://www.reanimatology.com/rmt/article/view/1811 |
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