New synthetic PEKK/bioceramic hybrids and their surface sulfonation counterparts have increased cellular osteogenic capacity and promoted osseointegration
PEKK has good biocompatibility as an implantable material, but its mechanical properties and osseointegration are not satisfied enough. Aluminum oxide (Al2O3) is highly wear-resistant, while hydroxyapatite (HA) is a biologically active. However all previous biocomposites made via mechanical mixing o...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-12-01
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| Series: | Materials & Design |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0264127522009054 |
| _version_ | 1828265398163734528 |
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| author | Pengzhen Gu Zhijing Wen Lifeng Bao Yibin Wang Pengrong Ouyang Teng Lu Jialiang Li Jiaxi Li Meng Jiang Na Li Xiangcheng Zhang Jian Wu Xijing He |
| author_facet | Pengzhen Gu Zhijing Wen Lifeng Bao Yibin Wang Pengrong Ouyang Teng Lu Jialiang Li Jiaxi Li Meng Jiang Na Li Xiangcheng Zhang Jian Wu Xijing He |
| author_sort | Pengzhen Gu |
| collection | DOAJ |
| description | PEKK has good biocompatibility as an implantable material, but its mechanical properties and osseointegration are not satisfied enough. Aluminum oxide (Al2O3) is highly wear-resistant, while hydroxyapatite (HA) is a biologically active. However all previous biocomposites made via mechanical mixing of these two bioceramic materials have not been able to meet clinical standards. The aim of this work is to resolve these problems by using newly synthesized bioceramic hybrids. In vitro and in vivo experiments have demonstrated that these new biomaterials have significantly improved biocompatibility and biomechanical properties when compared to neat PEKK. Surface sulfonation of the hybrids also leads to further improvements of these two properties. S-PEKK/Al2O3 and S-PEKK/HA promote MC3T3-E1 adhesion. PEKK/HA and S-PEKK/HA significantly promote cell proliferation by upregulating both mRNA and protein levels of cell cycle genes to facilitate cells shift from G0/G1 phase to S phase. PEKK/HA hybrids and S-PEKK/Al2O3 promote cell differentiation by upregulating the expression of osteogenic differentiation genes and proteins. PEKK/HA, S-PEKK/Al2O3 and S-PEKK/HA are able to strongly bind to the new bone and exhibit good in vivo osseointegration properties. We conclude that PEKK/HA, S-PEKK/Al2O3 and S-PEKK/HA show good osteogenic ability, especially S-PEKK/HA, and could be promising materials for bone implants. |
| first_indexed | 2024-04-13T04:38:46Z |
| format | Article |
| id | doaj.art-52a8931aa9a64eecadd33854c3651a16 |
| institution | Directory Open Access Journal |
| issn | 0264-1275 |
| language | English |
| last_indexed | 2024-04-13T04:38:46Z |
| publishDate | 2022-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Materials & Design |
| spelling | doaj.art-52a8931aa9a64eecadd33854c3651a162022-12-22T03:02:04ZengElsevierMaterials & Design0264-12752022-12-01224111283New synthetic PEKK/bioceramic hybrids and their surface sulfonation counterparts have increased cellular osteogenic capacity and promoted osseointegrationPengzhen Gu0Zhijing Wen1Lifeng Bao2Yibin Wang3Pengrong Ouyang4Teng Lu5Jialiang Li6Jiaxi Li7Meng Jiang8Na Li9Xiangcheng Zhang10Jian Wu11Xijing He12Department of Orthopedics, The Second Affiliated Hospital of Xi’an Jiaotong University, Xi’an, Shaanxi 710004, ChinaDepartment of Orthopedics, The Second Affiliated Hospital of Xi’an Jiaotong University, Xi’an, Shaanxi 710004, ChinaXiangcheng Medical Materials Technology, Ltd, Suzhou, Jiangsu 215123, ChinaDepartment of Orthopedics, The Second Affiliated Hospital of Xi’an Jiaotong University, Xi’an, Shaanxi 710004, ChinaDepartment of Orthopedics, The Second Affiliated Hospital of Xi’an Jiaotong University, Xi’an, Shaanxi 710004, ChinaDepartment of Orthopedics, The Second Affiliated Hospital of Xi’an Jiaotong University, Xi’an, Shaanxi 710004, ChinaDepartment of Orthopedics, The Second Affiliated Hospital of Xi’an Jiaotong University, Xi’an, Shaanxi 710004, ChinaDepartment of Orthopedics, The Second Affiliated Hospital of Xi’an Jiaotong University, Xi’an, Shaanxi 710004, ChinaDepartment of Spine Surgery, Xi’an International Medical Center Hospital, Xi’an, Shaanxi 710000, ChinaDepartment of Radiology, Xi'an Ninth Hospital, Xi’an, Shaanxi 710054, ChinaCambridge Victoria College, Cambridge Science Park, Cambridge CB4 OFN, UK; Corresponding authors.Laboratory of Multifunctional Nanomaterials and Smart Systems, Advanced Materials Division, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Suzhou 215123, China; Corresponding authors.Department of Orthopedics, The Second Affiliated Hospital of Xi’an Jiaotong University, Xi’an, Shaanxi 710004, China; Corresponding authors.PEKK has good biocompatibility as an implantable material, but its mechanical properties and osseointegration are not satisfied enough. Aluminum oxide (Al2O3) is highly wear-resistant, while hydroxyapatite (HA) is a biologically active. However all previous biocomposites made via mechanical mixing of these two bioceramic materials have not been able to meet clinical standards. The aim of this work is to resolve these problems by using newly synthesized bioceramic hybrids. In vitro and in vivo experiments have demonstrated that these new biomaterials have significantly improved biocompatibility and biomechanical properties when compared to neat PEKK. Surface sulfonation of the hybrids also leads to further improvements of these two properties. S-PEKK/Al2O3 and S-PEKK/HA promote MC3T3-E1 adhesion. PEKK/HA and S-PEKK/HA significantly promote cell proliferation by upregulating both mRNA and protein levels of cell cycle genes to facilitate cells shift from G0/G1 phase to S phase. PEKK/HA hybrids and S-PEKK/Al2O3 promote cell differentiation by upregulating the expression of osteogenic differentiation genes and proteins. PEKK/HA, S-PEKK/Al2O3 and S-PEKK/HA are able to strongly bind to the new bone and exhibit good in vivo osseointegration properties. We conclude that PEKK/HA, S-PEKK/Al2O3 and S-PEKK/HA show good osteogenic ability, especially S-PEKK/HA, and could be promising materials for bone implants.http://www.sciencedirect.com/science/article/pii/S0264127522009054PEKKHydroxyapatiteAluminaBioceramic hybridsOsseointegration |
| spellingShingle | Pengzhen Gu Zhijing Wen Lifeng Bao Yibin Wang Pengrong Ouyang Teng Lu Jialiang Li Jiaxi Li Meng Jiang Na Li Xiangcheng Zhang Jian Wu Xijing He New synthetic PEKK/bioceramic hybrids and their surface sulfonation counterparts have increased cellular osteogenic capacity and promoted osseointegration Materials & Design PEKK Hydroxyapatite Alumina Bioceramic hybrids Osseointegration |
| title | New synthetic PEKK/bioceramic hybrids and their surface sulfonation counterparts have increased cellular osteogenic capacity and promoted osseointegration |
| title_full | New synthetic PEKK/bioceramic hybrids and their surface sulfonation counterparts have increased cellular osteogenic capacity and promoted osseointegration |
| title_fullStr | New synthetic PEKK/bioceramic hybrids and their surface sulfonation counterparts have increased cellular osteogenic capacity and promoted osseointegration |
| title_full_unstemmed | New synthetic PEKK/bioceramic hybrids and their surface sulfonation counterparts have increased cellular osteogenic capacity and promoted osseointegration |
| title_short | New synthetic PEKK/bioceramic hybrids and their surface sulfonation counterparts have increased cellular osteogenic capacity and promoted osseointegration |
| title_sort | new synthetic pekk bioceramic hybrids and their surface sulfonation counterparts have increased cellular osteogenic capacity and promoted osseointegration |
| topic | PEKK Hydroxyapatite Alumina Bioceramic hybrids Osseointegration |
| url | http://www.sciencedirect.com/science/article/pii/S0264127522009054 |
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