Metastasis inhibition by BRMS1 and miR-31 replacement therapy in claudin-low cell lines
<em><strong>Objective(s):</strong></em> The growing trend of research demonstrates that dynamic expression of two metastasis repressor classes (metastasis suppressor genes and anti-metastatic miRNA) has a close relationship with tumor invasion and metastasis. Using different...
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| Format: | Article |
| Language: | English |
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Mashhad University of Medical Sciences
2020-02-01
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| Series: | Iranian Journal of Basic Medical Sciences |
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| Online Access: | http://ijbms.mums.ac.ir/article_14288_f3e7a762440d6352f0c5afff584f1725.pdf |
| _version_ | 1811277807432499200 |
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| author | Samila Farokhimanesh Mehdi Forouzandeh Moghadam Marzieh Ebrahimi |
| author_facet | Samila Farokhimanesh Mehdi Forouzandeh Moghadam Marzieh Ebrahimi |
| author_sort | Samila Farokhimanesh |
| collection | DOAJ |
| description | <em><strong>Objective(s):</strong></em> The growing trend of research demonstrates that dynamic expression of two metastasis repressor classes (metastasis suppressor genes and anti-metastatic miRNA) has a close relationship with tumor invasion and metastasis. Using different strategies, it was revealed that cellular levels of miR-31 and Breast cancer Metastasis Suppressor1 (BRMS1) protein, which are among the most significant modulators of metastasis, have a correlation with the cell’s capability for invading and metastasizing; cells containing higher levels of miR-31 or BRMS1 were less metastatic. This project was carried out to determine whether the combinations of miR-31 and BRMS1 genes are able to enhance the capability of repressing the claudin-low breast cancer cell (MDA-MB-231) invasion. Materials and <em><strong>Methods:</strong></em> This study used a restoration-based approach by miR-31 mimic and optimized BRMS1 gene sequences, which were cloned into a chimeric construct and transfected to the MDA-M231cells. <br /><em><strong>Results:</strong></em> Our data revealed that the simultaneous expression of anti-metastasis miR and metastasis suppressor might inhibit migration and invasion in MDA-MB-231 cells efficiently.<br /><em><strong>Conclusion:</strong></em> This combinatorial use of anti-metastatic miR and gene suggests a new therapeutic intervention for metastasis inhibition in MDA-MB-231. |
| first_indexed | 2024-04-13T00:23:23Z |
| format | Article |
| id | doaj.art-52aad7ccdb6943e9bbc88ebafdaf18c3 |
| institution | Directory Open Access Journal |
| issn | 2008-3866 2008-3874 |
| language | English |
| last_indexed | 2024-04-13T00:23:23Z |
| publishDate | 2020-02-01 |
| publisher | Mashhad University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Basic Medical Sciences |
| spelling | doaj.art-52aad7ccdb6943e9bbc88ebafdaf18c32022-12-22T03:10:41ZengMashhad University of Medical SciencesIranian Journal of Basic Medical Sciences2008-38662008-38742020-02-0123226427010.22038/ijbms.2019.35674.850014288Metastasis inhibition by BRMS1 and miR-31 replacement therapy in claudin-low cell linesSamila Farokhimanesh0Mehdi Forouzandeh Moghadam1Marzieh Ebrahimi2Department of Medical Biotechnology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran|Department of Biotechnology, Science and Research Branch, Islamic Azad Unversity, Tehran, IranDepartment of Medical Biotechnology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, IranDepartment of Stem Cells and Developmental Biology at Cell Science Research Center, Royan Institute for StemCell Biology and Technology, ACECR, Tehran, Iran<em><strong>Objective(s):</strong></em> The growing trend of research demonstrates that dynamic expression of two metastasis repressor classes (metastasis suppressor genes and anti-metastatic miRNA) has a close relationship with tumor invasion and metastasis. Using different strategies, it was revealed that cellular levels of miR-31 and Breast cancer Metastasis Suppressor1 (BRMS1) protein, which are among the most significant modulators of metastasis, have a correlation with the cell’s capability for invading and metastasizing; cells containing higher levels of miR-31 or BRMS1 were less metastatic. This project was carried out to determine whether the combinations of miR-31 and BRMS1 genes are able to enhance the capability of repressing the claudin-low breast cancer cell (MDA-MB-231) invasion. Materials and <em><strong>Methods:</strong></em> This study used a restoration-based approach by miR-31 mimic and optimized BRMS1 gene sequences, which were cloned into a chimeric construct and transfected to the MDA-M231cells. <br /><em><strong>Results:</strong></em> Our data revealed that the simultaneous expression of anti-metastasis miR and metastasis suppressor might inhibit migration and invasion in MDA-MB-231 cells efficiently.<br /><em><strong>Conclusion:</strong></em> This combinatorial use of anti-metastatic miR and gene suggests a new therapeutic intervention for metastasis inhibition in MDA-MB-231.http://ijbms.mums.ac.ir/article_14288_f3e7a762440d6352f0c5afff584f1725.pdfbreast cancerbrms1mir 31neoplasm metastasisreplacement therapy |
| spellingShingle | Samila Farokhimanesh Mehdi Forouzandeh Moghadam Marzieh Ebrahimi Metastasis inhibition by BRMS1 and miR-31 replacement therapy in claudin-low cell lines Iranian Journal of Basic Medical Sciences breast cancer brms1 mir 31 neoplasm metastasis replacement therapy |
| title | Metastasis inhibition by BRMS1 and miR-31 replacement therapy in claudin-low cell lines |
| title_full | Metastasis inhibition by BRMS1 and miR-31 replacement therapy in claudin-low cell lines |
| title_fullStr | Metastasis inhibition by BRMS1 and miR-31 replacement therapy in claudin-low cell lines |
| title_full_unstemmed | Metastasis inhibition by BRMS1 and miR-31 replacement therapy in claudin-low cell lines |
| title_short | Metastasis inhibition by BRMS1 and miR-31 replacement therapy in claudin-low cell lines |
| title_sort | metastasis inhibition by brms1 and mir 31 replacement therapy in claudin low cell lines |
| topic | breast cancer brms1 mir 31 neoplasm metastasis replacement therapy |
| url | http://ijbms.mums.ac.ir/article_14288_f3e7a762440d6352f0c5afff584f1725.pdf |
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