Comparative profiling of the transcriptional response to infection in two species of Drosophila by short-read cDNA sequencing
<p>Abstract</p> <p>Background</p> <p>Homology-based comparisons of the genes involved in innate immunity across many insect taxa with fully sequenced genomes has revealed a striking pattern of gene gain and loss, particularly among genes that encode proteins involved in...
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| Format: | Article |
| Language: | English |
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BMC
2009-06-01
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| Series: | BMC Genomics |
| Online Access: | http://www.biomedcentral.com/1471-2164/10/259 |
| _version_ | 1818807219313967104 |
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| author | Sackton Timothy B Clark Andrew G |
| author_facet | Sackton Timothy B Clark Andrew G |
| author_sort | Sackton Timothy B |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Homology-based comparisons of the genes involved in innate immunity across many insect taxa with fully sequenced genomes has revealed a striking pattern of gene gain and loss, particularly among genes that encode proteins involved in clearing pathogens (effectors). However, limited functional annotation in non-model systems has hindered understanding of evolutionary novelties in the insect innate immune system.</p> <p>Results</p> <p>We use short read sequencing technology (Illumina/Solexa) to compare the transcriptional response to infection between the well studied model system <it>Drosophila melanogaster </it>and the distantly related drosophilid <it>D. virilis</it>. We first demonstrate that Illumina/Solexa sequencing of cDNA from infected and uninfected <it>D. melanogaster </it>recapitulates previously published microarray studies of the transcriptional response to infection in this species, validating our approach. We then show that patterns of transcription of homologous genes differ considerably between <it>D. melanogaster </it>and <it>D. virilis</it>, and identify potential candidates for novel components of the <it>D. virilis </it>immune system based on transcriptional data. Finally, we use a proteomic approach to characterize the protein constituents of the <it>D. virilis </it>hemolymph and validate our transcriptional data.</p> <p>Conclusion</p> <p>These results suggest that the acquisition of novel components of the immune system, and particularly novel effector proteins, may be a common evolutionary phenomenon.</p> |
| first_indexed | 2024-12-18T19:22:11Z |
| format | Article |
| id | doaj.art-52ab6af308bb421b9876a99c0c59ab2c |
| institution | Directory Open Access Journal |
| issn | 1471-2164 |
| language | English |
| last_indexed | 2024-12-18T19:22:11Z |
| publishDate | 2009-06-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Genomics |
| spelling | doaj.art-52ab6af308bb421b9876a99c0c59ab2c2022-12-21T20:55:58ZengBMCBMC Genomics1471-21642009-06-0110125910.1186/1471-2164-10-259Comparative profiling of the transcriptional response to infection in two species of Drosophila by short-read cDNA sequencingSackton Timothy BClark Andrew G<p>Abstract</p> <p>Background</p> <p>Homology-based comparisons of the genes involved in innate immunity across many insect taxa with fully sequenced genomes has revealed a striking pattern of gene gain and loss, particularly among genes that encode proteins involved in clearing pathogens (effectors). However, limited functional annotation in non-model systems has hindered understanding of evolutionary novelties in the insect innate immune system.</p> <p>Results</p> <p>We use short read sequencing technology (Illumina/Solexa) to compare the transcriptional response to infection between the well studied model system <it>Drosophila melanogaster </it>and the distantly related drosophilid <it>D. virilis</it>. We first demonstrate that Illumina/Solexa sequencing of cDNA from infected and uninfected <it>D. melanogaster </it>recapitulates previously published microarray studies of the transcriptional response to infection in this species, validating our approach. We then show that patterns of transcription of homologous genes differ considerably between <it>D. melanogaster </it>and <it>D. virilis</it>, and identify potential candidates for novel components of the <it>D. virilis </it>immune system based on transcriptional data. Finally, we use a proteomic approach to characterize the protein constituents of the <it>D. virilis </it>hemolymph and validate our transcriptional data.</p> <p>Conclusion</p> <p>These results suggest that the acquisition of novel components of the immune system, and particularly novel effector proteins, may be a common evolutionary phenomenon.</p>http://www.biomedcentral.com/1471-2164/10/259 |
| spellingShingle | Sackton Timothy B Clark Andrew G Comparative profiling of the transcriptional response to infection in two species of Drosophila by short-read cDNA sequencing BMC Genomics |
| title | Comparative profiling of the transcriptional response to infection in two species of Drosophila by short-read cDNA sequencing |
| title_full | Comparative profiling of the transcriptional response to infection in two species of Drosophila by short-read cDNA sequencing |
| title_fullStr | Comparative profiling of the transcriptional response to infection in two species of Drosophila by short-read cDNA sequencing |
| title_full_unstemmed | Comparative profiling of the transcriptional response to infection in two species of Drosophila by short-read cDNA sequencing |
| title_short | Comparative profiling of the transcriptional response to infection in two species of Drosophila by short-read cDNA sequencing |
| title_sort | comparative profiling of the transcriptional response to infection in two species of drosophila by short read cdna sequencing |
| url | http://www.biomedcentral.com/1471-2164/10/259 |
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