The Anti-Oxidative and Anti-Neuroinflammatory Effects of <i>Sargassum horneri</i> by Heme Oxygenase-1 Induction in BV2 and HT22 Cells
<i>Sargassum horneri</i> is used as a traditional medicinal agent and exhibits various pharmacological effects. In this study, we found that the 70% EtOH extract contained 34.37 ± 0.75 μg/mg fucosterol. We tested the antioxidant activities of the 70% EtOH extracts and their fractions. Th...
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MDPI AG
2021-05-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/10/6/859 |
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| author | Wonmin Ko Hwan Lee Nayeon Kim Hee Geun Jo Eun-Rhan Woo Kyounghoon Lee Young Seok Han Sang Rul Park Ginnae Ahn Sun Hee Cheong Dong-Sung Lee |
| author_facet | Wonmin Ko Hwan Lee Nayeon Kim Hee Geun Jo Eun-Rhan Woo Kyounghoon Lee Young Seok Han Sang Rul Park Ginnae Ahn Sun Hee Cheong Dong-Sung Lee |
| author_sort | Wonmin Ko |
| collection | DOAJ |
| description | <i>Sargassum horneri</i> is used as a traditional medicinal agent and exhibits various pharmacological effects. In this study, we found that the 70% EtOH extract contained 34.37 ± 0.75 μg/mg fucosterol. We tested the antioxidant activities of the 70% EtOH extracts and their fractions. The CH<sub>2</sub>Cl<sub>2</sub>-soluble fraction showed the strongest DPPH and ABTS radical scavenging activities. Next, we evaluated the anti-neuroinflammatory effects of <i>S. horneri</i> on lipopolysaccharide (LPS)-stimulated BV2 cells. Pretreatment with the extract and fractions suppressed LPS-induced production of nitric oxide (NO) in BV2 cells. The 70% EtOH, CH<sub>2</sub>Cl<sub>2</sub>-soluble fraction, and water-soluble fraction inhibited the production of prostaglandin E2, interleukin-6, and tumor necrosis factor-α, as well as markedly blocking LPS-induced expression of inducible NO synthase and cyclooxygenase-2 via inactivation of the nuclear factor-kappa B pathway. In addition, the CH<sub>2</sub>Cl<sub>2</sub>-soluble fraction showed the most remarkable heme oxygenase (HO)-1 expression effects and increased nuclear erythroid 2-related factor translocation in the nucleus. In HT22 cells, the CH<sub>2</sub>Cl<sub>2</sub>-soluble fraction inhibited cell damage and ROS production caused by glutamate via the regulation of HO-1. Therefore, CH<sub>2</sub>Cl<sub>2</sub>-soluble fractions of <i>S. horneri</i> can attenuate oxidative action and neuroinflammatory responses via HO-1 induction, demonstrating their potential in the treatment of neuroinflammatory diseases. |
| first_indexed | 2024-03-10T10:59:22Z |
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| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-10T10:59:22Z |
| publishDate | 2021-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-52ac24e4b97a4ba7aca24c448d2176472023-11-21T21:36:30ZengMDPI AGAntioxidants2076-39212021-05-0110685910.3390/antiox10060859The Anti-Oxidative and Anti-Neuroinflammatory Effects of <i>Sargassum horneri</i> by Heme Oxygenase-1 Induction in BV2 and HT22 CellsWonmin Ko0Hwan Lee1Nayeon Kim2Hee Geun Jo3Eun-Rhan Woo4Kyounghoon Lee5Young Seok Han6Sang Rul Park7Ginnae Ahn8Sun Hee Cheong9Dong-Sung Lee10Department of Marine Bio-Food Sciences, Chonnam National University, Yeosu 59626, KoreaCollege of Pharmacy, Chosun University, Dong-gu, Gwangju 61452, KoreaCollege of Pharmacy, Chosun University, Dong-gu, Gwangju 61452, KoreaDepartment of Marine Bio-Food Sciences, Chonnam National University, Yeosu 59626, KoreaCollege of Pharmacy, Chosun University, Dong-gu, Gwangju 61452, KoreaDivision of Fisheries Science, Chonnam National University, Yeosu 59626, KoreaNeo Environmental Business Co., Daewoo Technopark, Doyak-ro, Bucheon 14523, KoreaEstuarine and Coastal Ecology Laboratory, Department of Marine Life Sciences, Jeju National University, Jeju 63243, KoreaDepartment of Marine Bio-Food Sciences, Chonnam National University, Yeosu 59626, KoreaDepartment of Marine Bio-Food Sciences, Chonnam National University, Yeosu 59626, KoreaCollege of Pharmacy, Chosun University, Dong-gu, Gwangju 61452, Korea<i>Sargassum horneri</i> is used as a traditional medicinal agent and exhibits various pharmacological effects. In this study, we found that the 70% EtOH extract contained 34.37 ± 0.75 μg/mg fucosterol. We tested the antioxidant activities of the 70% EtOH extracts and their fractions. The CH<sub>2</sub>Cl<sub>2</sub>-soluble fraction showed the strongest DPPH and ABTS radical scavenging activities. Next, we evaluated the anti-neuroinflammatory effects of <i>S. horneri</i> on lipopolysaccharide (LPS)-stimulated BV2 cells. Pretreatment with the extract and fractions suppressed LPS-induced production of nitric oxide (NO) in BV2 cells. The 70% EtOH, CH<sub>2</sub>Cl<sub>2</sub>-soluble fraction, and water-soluble fraction inhibited the production of prostaglandin E2, interleukin-6, and tumor necrosis factor-α, as well as markedly blocking LPS-induced expression of inducible NO synthase and cyclooxygenase-2 via inactivation of the nuclear factor-kappa B pathway. In addition, the CH<sub>2</sub>Cl<sub>2</sub>-soluble fraction showed the most remarkable heme oxygenase (HO)-1 expression effects and increased nuclear erythroid 2-related factor translocation in the nucleus. In HT22 cells, the CH<sub>2</sub>Cl<sub>2</sub>-soluble fraction inhibited cell damage and ROS production caused by glutamate via the regulation of HO-1. Therefore, CH<sub>2</sub>Cl<sub>2</sub>-soluble fractions of <i>S. horneri</i> can attenuate oxidative action and neuroinflammatory responses via HO-1 induction, demonstrating their potential in the treatment of neuroinflammatory diseases.https://www.mdpi.com/2076-3921/10/6/859<i>Sargassum horneri</i>CH<sub>2</sub>Cl<sub>2</sub>-soluble fractionantioxidant activityanti-neuroinflammatory effectsheme oxygenase-1BV2 cells |
| spellingShingle | Wonmin Ko Hwan Lee Nayeon Kim Hee Geun Jo Eun-Rhan Woo Kyounghoon Lee Young Seok Han Sang Rul Park Ginnae Ahn Sun Hee Cheong Dong-Sung Lee The Anti-Oxidative and Anti-Neuroinflammatory Effects of <i>Sargassum horneri</i> by Heme Oxygenase-1 Induction in BV2 and HT22 Cells Antioxidants <i>Sargassum horneri</i> CH<sub>2</sub>Cl<sub>2</sub>-soluble fraction antioxidant activity anti-neuroinflammatory effects heme oxygenase-1 BV2 cells |
| title | The Anti-Oxidative and Anti-Neuroinflammatory Effects of <i>Sargassum horneri</i> by Heme Oxygenase-1 Induction in BV2 and HT22 Cells |
| title_full | The Anti-Oxidative and Anti-Neuroinflammatory Effects of <i>Sargassum horneri</i> by Heme Oxygenase-1 Induction in BV2 and HT22 Cells |
| title_fullStr | The Anti-Oxidative and Anti-Neuroinflammatory Effects of <i>Sargassum horneri</i> by Heme Oxygenase-1 Induction in BV2 and HT22 Cells |
| title_full_unstemmed | The Anti-Oxidative and Anti-Neuroinflammatory Effects of <i>Sargassum horneri</i> by Heme Oxygenase-1 Induction in BV2 and HT22 Cells |
| title_short | The Anti-Oxidative and Anti-Neuroinflammatory Effects of <i>Sargassum horneri</i> by Heme Oxygenase-1 Induction in BV2 and HT22 Cells |
| title_sort | anti oxidative and anti neuroinflammatory effects of i sargassum horneri i by heme oxygenase 1 induction in bv2 and ht22 cells |
| topic | <i>Sargassum horneri</i> CH<sub>2</sub>Cl<sub>2</sub>-soluble fraction antioxidant activity anti-neuroinflammatory effects heme oxygenase-1 BV2 cells |
| url | https://www.mdpi.com/2076-3921/10/6/859 |
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