The Identification of Prohibitin in the Rat Heart Mitochondria in Heart Failure
Mitochondria are considered the main organelles in the cell. They play an important role in both normal and abnormal heart function. There is a supramolecular organization between the complexes of the respiratory chain (supercomplexes (SCs)), which are involved in mitochondrial respiration. Prohibit...
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| Format: | Article |
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MDPI AG
2021-11-01
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| Series: | Biomedicines |
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| Online Access: | https://www.mdpi.com/2227-9059/9/12/1793 |
| _version_ | 1797506506522886144 |
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| author | Yulia Baburina Roman Krestinin Irina Odinokova Irina Fadeeva Linda Sotnikova Olga Krestinina |
| author_facet | Yulia Baburina Roman Krestinin Irina Odinokova Irina Fadeeva Linda Sotnikova Olga Krestinina |
| author_sort | Yulia Baburina |
| collection | DOAJ |
| description | Mitochondria are considered the main organelles in the cell. They play an important role in both normal and abnormal heart function. There is a supramolecular organization between the complexes of the respiratory chain (supercomplexes (SCs)), which are involved in mitochondrial respiration. Prohibitins (PHBs) participate in the regulation of oxidative phosphorylation (OXPHOS) activity and interact with some subunits of the OXPHOS complexes. In this study, we identified a protein whose level was decreased in the mitochondria of the heart in rats with heart failure. This protein was PHB. Isoproterenol (ISO) has been used as a compound to induce heart failure in rats. We observed that astaxanthin (AX) increased the content of PHB in rat heart mitochondria isolated from ISO-injected rats. Since it is known that PHB forms complexes with some mitochondrial proteins and proteins that are part of the complexes of the respiratory chain, the change in the levels of these proteins was investigated under our experimental conditions. We hypothesized that PHB may be a target for the protective action of AX. |
| first_indexed | 2024-03-10T04:33:38Z |
| format | Article |
| id | doaj.art-52ac6be7ab5e486ba4df6b83803dae40 |
| institution | Directory Open Access Journal |
| issn | 2227-9059 |
| language | English |
| last_indexed | 2024-03-10T04:33:38Z |
| publishDate | 2021-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomedicines |
| spelling | doaj.art-52ac6be7ab5e486ba4df6b83803dae402023-11-23T03:55:39ZengMDPI AGBiomedicines2227-90592021-11-01912179310.3390/biomedicines9121793The Identification of Prohibitin in the Rat Heart Mitochondria in Heart FailureYulia Baburina0Roman Krestinin1Irina Odinokova2Irina Fadeeva3Linda Sotnikova4Olga Krestinina5Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, 142290 Pushchino, Moscow Region, RussiaInstitute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, 142290 Pushchino, Moscow Region, RussiaInstitute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, 142290 Pushchino, Moscow Region, RussiaInstitute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, 142290 Pushchino, Moscow Region, RussiaInstitute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, 142290 Pushchino, Moscow Region, RussiaInstitute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, 142290 Pushchino, Moscow Region, RussiaMitochondria are considered the main organelles in the cell. They play an important role in both normal and abnormal heart function. There is a supramolecular organization between the complexes of the respiratory chain (supercomplexes (SCs)), which are involved in mitochondrial respiration. Prohibitins (PHBs) participate in the regulation of oxidative phosphorylation (OXPHOS) activity and interact with some subunits of the OXPHOS complexes. In this study, we identified a protein whose level was decreased in the mitochondria of the heart in rats with heart failure. This protein was PHB. Isoproterenol (ISO) has been used as a compound to induce heart failure in rats. We observed that astaxanthin (AX) increased the content of PHB in rat heart mitochondria isolated from ISO-injected rats. Since it is known that PHB forms complexes with some mitochondrial proteins and proteins that are part of the complexes of the respiratory chain, the change in the levels of these proteins was investigated under our experimental conditions. We hypothesized that PHB may be a target for the protective action of AX.https://www.mdpi.com/2227-9059/9/12/1793rat heart mitochondria (RHM)heart failure (HF)prohibitin (PHB)astaxantin (AX)isoproterenol (ISO)oxidative stress |
| spellingShingle | Yulia Baburina Roman Krestinin Irina Odinokova Irina Fadeeva Linda Sotnikova Olga Krestinina The Identification of Prohibitin in the Rat Heart Mitochondria in Heart Failure Biomedicines rat heart mitochondria (RHM) heart failure (HF) prohibitin (PHB) astaxantin (AX) isoproterenol (ISO) oxidative stress |
| title | The Identification of Prohibitin in the Rat Heart Mitochondria in Heart Failure |
| title_full | The Identification of Prohibitin in the Rat Heart Mitochondria in Heart Failure |
| title_fullStr | The Identification of Prohibitin in the Rat Heart Mitochondria in Heart Failure |
| title_full_unstemmed | The Identification of Prohibitin in the Rat Heart Mitochondria in Heart Failure |
| title_short | The Identification of Prohibitin in the Rat Heart Mitochondria in Heart Failure |
| title_sort | identification of prohibitin in the rat heart mitochondria in heart failure |
| topic | rat heart mitochondria (RHM) heart failure (HF) prohibitin (PHB) astaxantin (AX) isoproterenol (ISO) oxidative stress |
| url | https://www.mdpi.com/2227-9059/9/12/1793 |
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