Circular RNA circRHOBTB3 is downregulated in hepatocellular carcinoma and suppresses cell proliferation by inhibiting miR-18a maturation
Abstract Background Circular RNA circRHOBTB3 has been characterized as a tumor suppressor in gastric cancer, while its role in hepatocellular carcinoma (HCC) is unknown. This study was carried out to analyze the role of circRHOBTB3 in HCC. Methods In this study, circRHOBTB3, mature miR-18a, and miR-...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2021-06-01
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| Series: | Infectious Agents and Cancer |
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| Online Access: | https://doi.org/10.1186/s13027-021-00384-1 |
| _version_ | 1818741098951999488 |
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| author | Gang Hu Shusen Zhai Sheng Yu Zhen Huang Ran Gao |
| author_facet | Gang Hu Shusen Zhai Sheng Yu Zhen Huang Ran Gao |
| author_sort | Gang Hu |
| collection | DOAJ |
| description | Abstract Background Circular RNA circRHOBTB3 has been characterized as a tumor suppressor in gastric cancer, while its role in hepatocellular carcinoma (HCC) is unknown. This study was carried out to analyze the role of circRHOBTB3 in HCC. Methods In this study, circRHOBTB3, mature miR-18a, and miR-18a precursor in HCC and paired non-cancer tissues were detected by RT-qPCR. The role of circRHOBTB3 in the production of mature miR-18a was explored by transfecting circRHOBTB3 expression vector into HCC cells, followed by RT-qPCR to determine the expression of mature miR-18a and miR-18a precursor. The role of circRHOBTB3 and miR-18a in HCC cell proliferation was studied using CCK-8 assay. Results CircRHOBTB3 was under-expressed in HCC compared to normal tissues. In HCC cells, circRHOBTB3 overexpression decreased mature miR-18a level but not miR-18a precursor. Cell proliferation analysis showed that circRHOBTB3 overexpression decreased cell proliferation while miR-18a overexpression increased cell proliferation. Moreover, circRHOBTB3 suppressed the role of miR-18a in cell proliferation. Conclusions CircRHOBTB3 is downregulated in HCC and may suppress cell proliferation by reducing miR-18a production. |
| first_indexed | 2024-12-18T01:51:14Z |
| format | Article |
| id | doaj.art-52ae0ecc84e741d286dfcd1ce94c113f |
| institution | Directory Open Access Journal |
| issn | 1750-9378 |
| language | English |
| last_indexed | 2024-12-18T01:51:14Z |
| publishDate | 2021-06-01 |
| publisher | BMC |
| record_format | Article |
| series | Infectious Agents and Cancer |
| spelling | doaj.art-52ae0ecc84e741d286dfcd1ce94c113f2022-12-21T21:25:02ZengBMCInfectious Agents and Cancer1750-93782021-06-011611710.1186/s13027-021-00384-1Circular RNA circRHOBTB3 is downregulated in hepatocellular carcinoma and suppresses cell proliferation by inhibiting miR-18a maturationGang Hu0Shusen Zhai1Sheng Yu2Zhen Huang3Ran Gao4Department of General Surgery, Strategic Support Force Characteristic Medical CenterDepartment of Oncology, Strategic Support Force Characteristic Medical CenterDivision of Hepatobiliopancreatic Surgery, Department of General Surgery, Nanfang Hospital, Southern Medical UniversityDepartment of General Surgery, Strategic Support Force Characteristic Medical CenterDepartment of General Surgery, Strategic Support Force Characteristic Medical CenterAbstract Background Circular RNA circRHOBTB3 has been characterized as a tumor suppressor in gastric cancer, while its role in hepatocellular carcinoma (HCC) is unknown. This study was carried out to analyze the role of circRHOBTB3 in HCC. Methods In this study, circRHOBTB3, mature miR-18a, and miR-18a precursor in HCC and paired non-cancer tissues were detected by RT-qPCR. The role of circRHOBTB3 in the production of mature miR-18a was explored by transfecting circRHOBTB3 expression vector into HCC cells, followed by RT-qPCR to determine the expression of mature miR-18a and miR-18a precursor. The role of circRHOBTB3 and miR-18a in HCC cell proliferation was studied using CCK-8 assay. Results CircRHOBTB3 was under-expressed in HCC compared to normal tissues. In HCC cells, circRHOBTB3 overexpression decreased mature miR-18a level but not miR-18a precursor. Cell proliferation analysis showed that circRHOBTB3 overexpression decreased cell proliferation while miR-18a overexpression increased cell proliferation. Moreover, circRHOBTB3 suppressed the role of miR-18a in cell proliferation. Conclusions CircRHOBTB3 is downregulated in HCC and may suppress cell proliferation by reducing miR-18a production.https://doi.org/10.1186/s13027-021-00384-1Hepatocellular carcinomacircRHOBTB3miR-18aPrecursor |
| spellingShingle | Gang Hu Shusen Zhai Sheng Yu Zhen Huang Ran Gao Circular RNA circRHOBTB3 is downregulated in hepatocellular carcinoma and suppresses cell proliferation by inhibiting miR-18a maturation Infectious Agents and Cancer Hepatocellular carcinoma circRHOBTB3 miR-18a Precursor |
| title | Circular RNA circRHOBTB3 is downregulated in hepatocellular carcinoma and suppresses cell proliferation by inhibiting miR-18a maturation |
| title_full | Circular RNA circRHOBTB3 is downregulated in hepatocellular carcinoma and suppresses cell proliferation by inhibiting miR-18a maturation |
| title_fullStr | Circular RNA circRHOBTB3 is downregulated in hepatocellular carcinoma and suppresses cell proliferation by inhibiting miR-18a maturation |
| title_full_unstemmed | Circular RNA circRHOBTB3 is downregulated in hepatocellular carcinoma and suppresses cell proliferation by inhibiting miR-18a maturation |
| title_short | Circular RNA circRHOBTB3 is downregulated in hepatocellular carcinoma and suppresses cell proliferation by inhibiting miR-18a maturation |
| title_sort | circular rna circrhobtb3 is downregulated in hepatocellular carcinoma and suppresses cell proliferation by inhibiting mir 18a maturation |
| topic | Hepatocellular carcinoma circRHOBTB3 miR-18a Precursor |
| url | https://doi.org/10.1186/s13027-021-00384-1 |
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