Short-term cold storage of sperm from six neotropical characiformes fishes
Sperm of the following Neotropical Characiformes fish species were tested for cold storage: Brycon lundii, Piaractus mesopotamicus, Leporinus elongatus, Leporinus friderici, Prochilodus lineatus and Prochilodus marggravii. Each sperm sample was split into two aliquots. The first was placed into a pl...
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| Format: | Article |
| Language: | English |
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Instituto de Tecnologia do Paraná (Tecpar)
2004-09-01
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| Series: | Brazilian Archives of Biology and Technology |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000500016 |
| _version_ | 1819259112862515200 |
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| author | Simone Marques Hugo Pereira Godinho |
| author_facet | Simone Marques Hugo Pereira Godinho |
| author_sort | Simone Marques |
| collection | DOAJ |
| description | Sperm of the following Neotropical Characiformes fish species were tested for cold storage: Brycon lundii, Piaractus mesopotamicus, Leporinus elongatus, Leporinus friderici, Prochilodus lineatus and Prochilodus marggravii. Each sperm sample was split into two aliquots. The first was placed into a plastic bag with air or oxygen and the second, in a plastic tube with air. The samples were maintained at temperatures between 1.7-4.9 ºC. The rate of sperm motility was estimated using a 50 mM NaCl solution as the activating solution. The shortest sperm storage duration (7 h) was recorded for L. friderici, when the sperm motility rate reached ~ 30%, whereas the longest duration (20 h) was obtained with the sperm of P. lineatus. A fertilisation test using Prochilodus marggravii sperm refrigerated for 8 h yielded 88-90% of viable embryos. The refrigerated storage method could be of practical applications, especially in fish reproductive management at hatchery stations.<br>O sêmen das seguintes espécies de Characiformes neotropicais foi testado para armazenamento em ambiente resfriado: Brycon lundii, Piaractus mesopotamicus, Leporinus elongatus, Leporinus friderici, Prochilodus lineatus e Prochilodus marggravii. Amostras de sêmen, obtidas por massagem da parede celômica, foram armazenadas em saco plástico com ar ou oxigênio ou em tubo plástico com ar, e mantidas resfriadas entre 1,7-4,9º C. A taxa de motilidade espermática foi estimada usando-se NaCl 50 mM como solução ativadora. O sêmen com menor duração de viabilidade (7 h) foi o de L. friderici, quando a taxa de motilidade espermática alcançou ~30%, enquanto que o de maior duração (20 h) foi o de P. lineatus. A fertilização de ovócitos utilizando sêmen refrigerado por 8 h de Prochilodus marggravii produziu 88-90% de embriões viáveis. O método de armazenamento desenvolvido neste trabalho tem aplicações práticas, especialmente no manejo reprodutivo de peixes em estações de piscicultura. |
| first_indexed | 2024-12-23T19:04:50Z |
| format | Article |
| id | doaj.art-52afe5626c5e443f903de27abb8bc415 |
| institution | Directory Open Access Journal |
| issn | 1516-8913 1678-4324 |
| language | English |
| last_indexed | 2024-12-23T19:04:50Z |
| publishDate | 2004-09-01 |
| publisher | Instituto de Tecnologia do Paraná (Tecpar) |
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| series | Brazilian Archives of Biology and Technology |
| spelling | doaj.art-52afe5626c5e443f903de27abb8bc4152022-12-21T17:34:37ZengInstituto de Tecnologia do Paraná (Tecpar)Brazilian Archives of Biology and Technology1516-89131678-43242004-09-0147579980410.1590/S1516-89132004000500016Short-term cold storage of sperm from six neotropical characiformes fishesSimone MarquesHugo Pereira GodinhoSperm of the following Neotropical Characiformes fish species were tested for cold storage: Brycon lundii, Piaractus mesopotamicus, Leporinus elongatus, Leporinus friderici, Prochilodus lineatus and Prochilodus marggravii. Each sperm sample was split into two aliquots. The first was placed into a plastic bag with air or oxygen and the second, in a plastic tube with air. The samples were maintained at temperatures between 1.7-4.9 ºC. The rate of sperm motility was estimated using a 50 mM NaCl solution as the activating solution. The shortest sperm storage duration (7 h) was recorded for L. friderici, when the sperm motility rate reached ~ 30%, whereas the longest duration (20 h) was obtained with the sperm of P. lineatus. A fertilisation test using Prochilodus marggravii sperm refrigerated for 8 h yielded 88-90% of viable embryos. The refrigerated storage method could be of practical applications, especially in fish reproductive management at hatchery stations.<br>O sêmen das seguintes espécies de Characiformes neotropicais foi testado para armazenamento em ambiente resfriado: Brycon lundii, Piaractus mesopotamicus, Leporinus elongatus, Leporinus friderici, Prochilodus lineatus e Prochilodus marggravii. Amostras de sêmen, obtidas por massagem da parede celômica, foram armazenadas em saco plástico com ar ou oxigênio ou em tubo plástico com ar, e mantidas resfriadas entre 1,7-4,9º C. A taxa de motilidade espermática foi estimada usando-se NaCl 50 mM como solução ativadora. O sêmen com menor duração de viabilidade (7 h) foi o de L. friderici, quando a taxa de motilidade espermática alcançou ~30%, enquanto que o de maior duração (20 h) foi o de P. lineatus. A fertilização de ovócitos utilizando sêmen refrigerado por 8 h de Prochilodus marggravii produziu 88-90% de embriões viáveis. O método de armazenamento desenvolvido neste trabalho tem aplicações práticas, especialmente no manejo reprodutivo de peixes em estações de piscicultura.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000500016Cool storage of spermsperm motilitysemenNeotropical fishes |
| spellingShingle | Simone Marques Hugo Pereira Godinho Short-term cold storage of sperm from six neotropical characiformes fishes Brazilian Archives of Biology and Technology Cool storage of sperm sperm motility semen Neotropical fishes |
| title | Short-term cold storage of sperm from six neotropical characiformes fishes |
| title_full | Short-term cold storage of sperm from six neotropical characiformes fishes |
| title_fullStr | Short-term cold storage of sperm from six neotropical characiformes fishes |
| title_full_unstemmed | Short-term cold storage of sperm from six neotropical characiformes fishes |
| title_short | Short-term cold storage of sperm from six neotropical characiformes fishes |
| title_sort | short term cold storage of sperm from six neotropical characiformes fishes |
| topic | Cool storage of sperm sperm motility semen Neotropical fishes |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000500016 |
| work_keys_str_mv | AT simonemarques shorttermcoldstorageofspermfromsixneotropicalcharaciformesfishes AT hugopereiragodinho shorttermcoldstorageofspermfromsixneotropicalcharaciformesfishes |