Leonurus japonicus Houtt Attenuates Nonalcoholic Fatty Liver Disease in Free Fatty Acid-Induced HepG2 Cells and Mice Fed a High-Fat Diet
We investigated the effects of a Leonurus japonicus ethanol extract (LJE) on nonalcoholic fatty liver disease (NAFLD). An in vitro model of hepatic steatosis was treated with 1 mM free fatty acid (FFA) in HepG2 cells. An in vivo NAFLD model was established using C57BL/6 mice fed a high-fat diet (HFD...
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MDPI AG
2017-12-01
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| Series: | Nutrients |
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| Online Access: | https://www.mdpi.com/2072-6643/10/1/20 |
| _version_ | 1811282018725527552 |
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| author | Mi-Ra Lee Kwang Il Park Jin Yeul Ma |
| author_facet | Mi-Ra Lee Kwang Il Park Jin Yeul Ma |
| author_sort | Mi-Ra Lee |
| collection | DOAJ |
| description | We investigated the effects of a Leonurus japonicus ethanol extract (LJE) on nonalcoholic fatty liver disease (NAFLD). An in vitro model of hepatic steatosis was treated with 1 mM free fatty acid (FFA) in HepG2 cells. An in vivo NAFLD model was established using C57BL/6 mice fed a high-fat diet (HFD) and administered LJE (100 or 200 mg/kg) orally for 14 weeks. LJE treatment suppressed lipid accumulation and intracellular triglyceride levels significantly in a concentration-dependent manner in HepG2 cells. Moreover, LJE significantly reduced the expression of sterol regulatory element binding protein 1-c, and its downstream genes, which are associated with lipogenesis, in HepG2 cells. In HFD-fed mice, LJE treatment decreased body weight significantly and decreased serum alanine transaminase levels to normal values, concurrent with a decrease in hepatic lipid accumulation. Furthermore, LJE supplementation ameliorated insulin sensitivity by decreasing serum glucose and insulin levels. LJE improved hepatic steatosis by increasing the expression of phosphorylated AMP-activated protein kinase and peroxisome proliferator-activated receptor-α in HFD-fed mice and FFA-treated HepG2 cells. The results suggested that LJE might be a potential therapeutic agent to treat NAFLD. |
| first_indexed | 2024-04-13T01:44:08Z |
| format | Article |
| id | doaj.art-52b0cc6eef6843918ef9b43fe35b67c3 |
| institution | Directory Open Access Journal |
| issn | 2072-6643 |
| language | English |
| last_indexed | 2024-04-13T01:44:08Z |
| publishDate | 2017-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nutrients |
| spelling | doaj.art-52b0cc6eef6843918ef9b43fe35b67c32022-12-22T03:08:05ZengMDPI AGNutrients2072-66432017-12-011012010.3390/nu10010020nu10010020Leonurus japonicus Houtt Attenuates Nonalcoholic Fatty Liver Disease in Free Fatty Acid-Induced HepG2 Cells and Mice Fed a High-Fat DietMi-Ra Lee0Kwang Il Park1Jin Yeul Ma2Korea Institute of Oriental Medicine, 70 Cheomdan-Ro, Dong-Gu, Daegu 41062, KoreaKorea Institute of Oriental Medicine, 70 Cheomdan-Ro, Dong-Gu, Daegu 41062, KoreaKorea Institute of Oriental Medicine, 70 Cheomdan-Ro, Dong-Gu, Daegu 41062, KoreaWe investigated the effects of a Leonurus japonicus ethanol extract (LJE) on nonalcoholic fatty liver disease (NAFLD). An in vitro model of hepatic steatosis was treated with 1 mM free fatty acid (FFA) in HepG2 cells. An in vivo NAFLD model was established using C57BL/6 mice fed a high-fat diet (HFD) and administered LJE (100 or 200 mg/kg) orally for 14 weeks. LJE treatment suppressed lipid accumulation and intracellular triglyceride levels significantly in a concentration-dependent manner in HepG2 cells. Moreover, LJE significantly reduced the expression of sterol regulatory element binding protein 1-c, and its downstream genes, which are associated with lipogenesis, in HepG2 cells. In HFD-fed mice, LJE treatment decreased body weight significantly and decreased serum alanine transaminase levels to normal values, concurrent with a decrease in hepatic lipid accumulation. Furthermore, LJE supplementation ameliorated insulin sensitivity by decreasing serum glucose and insulin levels. LJE improved hepatic steatosis by increasing the expression of phosphorylated AMP-activated protein kinase and peroxisome proliferator-activated receptor-α in HFD-fed mice and FFA-treated HepG2 cells. The results suggested that LJE might be a potential therapeutic agent to treat NAFLD.https://www.mdpi.com/2072-6643/10/1/20free fatty acidsHepG2 cellshigh-fat dietobesityLeonurus japonicusnonalcoholic fatty liver disease |
| spellingShingle | Mi-Ra Lee Kwang Il Park Jin Yeul Ma Leonurus japonicus Houtt Attenuates Nonalcoholic Fatty Liver Disease in Free Fatty Acid-Induced HepG2 Cells and Mice Fed a High-Fat Diet Nutrients free fatty acids HepG2 cells high-fat diet obesity Leonurus japonicus nonalcoholic fatty liver disease |
| title | Leonurus japonicus Houtt Attenuates Nonalcoholic Fatty Liver Disease in Free Fatty Acid-Induced HepG2 Cells and Mice Fed a High-Fat Diet |
| title_full | Leonurus japonicus Houtt Attenuates Nonalcoholic Fatty Liver Disease in Free Fatty Acid-Induced HepG2 Cells and Mice Fed a High-Fat Diet |
| title_fullStr | Leonurus japonicus Houtt Attenuates Nonalcoholic Fatty Liver Disease in Free Fatty Acid-Induced HepG2 Cells and Mice Fed a High-Fat Diet |
| title_full_unstemmed | Leonurus japonicus Houtt Attenuates Nonalcoholic Fatty Liver Disease in Free Fatty Acid-Induced HepG2 Cells and Mice Fed a High-Fat Diet |
| title_short | Leonurus japonicus Houtt Attenuates Nonalcoholic Fatty Liver Disease in Free Fatty Acid-Induced HepG2 Cells and Mice Fed a High-Fat Diet |
| title_sort | leonurus japonicus houtt attenuates nonalcoholic fatty liver disease in free fatty acid induced hepg2 cells and mice fed a high fat diet |
| topic | free fatty acids HepG2 cells high-fat diet obesity Leonurus japonicus nonalcoholic fatty liver disease |
| url | https://www.mdpi.com/2072-6643/10/1/20 |
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