In vitro incorporation of radiolabeled cholesteryl esters into high and low density lipoproteins.
We have developed and validated a method for in vitro incorporation of radiolabeled cholesteryl esters into low density (LDL) and high density lipoproteins (HDL). Radiolabeled cholesteryl esters dissolved in absolute ethanol were mixed with LDL or HDL in the presence of lipoprotein-deficient serum (...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Elsevier
1989-11-01
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| Series: | Journal of Lipid Research |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520382286 |
| _version_ | 1819058267947532288 |
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| author | A H Terpstra R J Nicolosi P N Herbert |
| author_facet | A H Terpstra R J Nicolosi P N Herbert |
| author_sort | A H Terpstra |
| collection | DOAJ |
| description | We have developed and validated a method for in vitro incorporation of radiolabeled cholesteryl esters into low density (LDL) and high density lipoproteins (HDL). Radiolabeled cholesteryl esters dissolved in absolute ethanol were mixed with LDL or HDL in the presence of lipoprotein-deficient serum (LPDS) as a source of core lipid transfer activity. The efficiency of incorporation was dependent on: a) the core lipid transfer activity and quantity of LPDS, b) the mass of added radiolabeled cholesteryl esters, c) the length of incubation, and d) the amount of acceptor lipoprotein cholesterol. The tracer incorporation was documented by repeat density gradient ultracentrifugation, agarose gel electrophoresis, and precipitation with heparin-MnCl2. The radiolabeling conditions did not affect the following properties of the lipoproteins: 1) chemical composition, 2) electrophoretic mobility on agarose gels, 3) hydrated density, 4) distribution of apoproteins on SDS gels, 5) plasma clearance rates, and 6) immunoprecipitability of HDL apoproteins A-I and A-II. Rat HDL containing radiolabeled cholesteryl esters incorporated in vitro had plasma disappearance rates identical to HDL radiolabeled in vivo. |
| first_indexed | 2024-12-21T13:52:30Z |
| format | Article |
| id | doaj.art-52b116b731f9455e8cc7912b0c463d74 |
| institution | Directory Open Access Journal |
| issn | 0022-2275 |
| language | English |
| last_indexed | 2024-12-21T13:52:30Z |
| publishDate | 1989-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Lipid Research |
| spelling | doaj.art-52b116b731f9455e8cc7912b0c463d742022-12-21T19:01:40ZengElsevierJournal of Lipid Research0022-22751989-11-01301116631671In vitro incorporation of radiolabeled cholesteryl esters into high and low density lipoproteins.A H Terpstra0R J Nicolosi1P N Herbert2Brown University Program in Medicine, Miriam Hospital, Providence, RI 02906.Brown University Program in Medicine, Miriam Hospital, Providence, RI 02906.Brown University Program in Medicine, Miriam Hospital, Providence, RI 02906.We have developed and validated a method for in vitro incorporation of radiolabeled cholesteryl esters into low density (LDL) and high density lipoproteins (HDL). Radiolabeled cholesteryl esters dissolved in absolute ethanol were mixed with LDL or HDL in the presence of lipoprotein-deficient serum (LPDS) as a source of core lipid transfer activity. The efficiency of incorporation was dependent on: a) the core lipid transfer activity and quantity of LPDS, b) the mass of added radiolabeled cholesteryl esters, c) the length of incubation, and d) the amount of acceptor lipoprotein cholesterol. The tracer incorporation was documented by repeat density gradient ultracentrifugation, agarose gel electrophoresis, and precipitation with heparin-MnCl2. The radiolabeling conditions did not affect the following properties of the lipoproteins: 1) chemical composition, 2) electrophoretic mobility on agarose gels, 3) hydrated density, 4) distribution of apoproteins on SDS gels, 5) plasma clearance rates, and 6) immunoprecipitability of HDL apoproteins A-I and A-II. Rat HDL containing radiolabeled cholesteryl esters incorporated in vitro had plasma disappearance rates identical to HDL radiolabeled in vivo.http://www.sciencedirect.com/science/article/pii/S0022227520382286 |
| spellingShingle | A H Terpstra R J Nicolosi P N Herbert In vitro incorporation of radiolabeled cholesteryl esters into high and low density lipoproteins. Journal of Lipid Research |
| title | In vitro incorporation of radiolabeled cholesteryl esters into high and low density lipoproteins. |
| title_full | In vitro incorporation of radiolabeled cholesteryl esters into high and low density lipoproteins. |
| title_fullStr | In vitro incorporation of radiolabeled cholesteryl esters into high and low density lipoproteins. |
| title_full_unstemmed | In vitro incorporation of radiolabeled cholesteryl esters into high and low density lipoproteins. |
| title_short | In vitro incorporation of radiolabeled cholesteryl esters into high and low density lipoproteins. |
| title_sort | in vitro incorporation of radiolabeled cholesteryl esters into high and low density lipoproteins |
| url | http://www.sciencedirect.com/science/article/pii/S0022227520382286 |
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