Efficient pH and dissolved CO2 conditions for indoor and outdoor cultures of green alga Parachlorella
Efficient pH and dissolved CO2 conditions for indoor (50–450 mL scale) and outdoor (100–500 L scale) culture of a green alga BX1.5 strain that can produce useful intracellular lipids and extracellular polysaccharides were investigated for the first time in Parachlorella sp. The cultures harvested un...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-09-01
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| Series: | Frontiers in Bioengineering and Biotechnology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fbioe.2023.1233944/full |
| _version_ | 1827820867549134848 |
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| author | Akari Takagi Misato Nagao Yuya Uejima Daisaku Sasaki Munehiko Asayama Munehiko Asayama |
| author_facet | Akari Takagi Misato Nagao Yuya Uejima Daisaku Sasaki Munehiko Asayama Munehiko Asayama |
| author_sort | Akari Takagi |
| collection | DOAJ |
| description | Efficient pH and dissolved CO2 conditions for indoor (50–450 mL scale) and outdoor (100–500 L scale) culture of a green alga BX1.5 strain that can produce useful intracellular lipids and extracellular polysaccharides were investigated for the first time in Parachlorella sp. The cultures harvested under 26 different conditions were analysed for pH, dissolved CO2 concentration, and the biomass of extracellular polysaccharides. The BX1.5 strain could thrive in a wide range of initial medium pH ranging from 3 to 11 and produced valuable lipids such as C16:0, C18:2, and C18:3 under indoor and outdoor culture conditions when supplied with 2.0% dissolved CO2. Particularly, the acidic BG11 medium effectively increased the biomass of extracellular polysaccharides during short-term outdoor cultivation. The BG11 liquid medium also led to extracellular polysaccharide production, independent of acidity and alkalinity, proportional to the increase in total sugars derived from cells supplied with high CO2 concentrations. These results suggest Parachlorella as a promising strain for indoor and outdoor cultivation to produce valuable materials. |
| first_indexed | 2024-03-12T01:34:08Z |
| format | Article |
| id | doaj.art-52b63fed59674ef295213b962f2e1d1f |
| institution | Directory Open Access Journal |
| issn | 2296-4185 |
| language | English |
| last_indexed | 2024-03-12T01:34:08Z |
| publishDate | 2023-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj.art-52b63fed59674ef295213b962f2e1d1f2023-09-11T12:23:07ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852023-09-011110.3389/fbioe.2023.12339441233944Efficient pH and dissolved CO2 conditions for indoor and outdoor cultures of green alga ParachlorellaAkari Takagi0Misato Nagao1Yuya Uejima2Daisaku Sasaki3Munehiko Asayama4Munehiko Asayama5College of Agriculture, Ibaraki University, Ibaraki, JapanCollege of Agriculture, Ibaraki University, Ibaraki, JapanCollege of Agriculture, Ibaraki University, Ibaraki, JapanBioX Chemical Industries Co., Ltd., Hiroshima, JapanCollege of Agriculture, Ibaraki University, Ibaraki, JapanUnited Graduate School of Agricultural Science, Tokyo University of Agriculture and Technology, Tokyo, JapanEfficient pH and dissolved CO2 conditions for indoor (50–450 mL scale) and outdoor (100–500 L scale) culture of a green alga BX1.5 strain that can produce useful intracellular lipids and extracellular polysaccharides were investigated for the first time in Parachlorella sp. The cultures harvested under 26 different conditions were analysed for pH, dissolved CO2 concentration, and the biomass of extracellular polysaccharides. The BX1.5 strain could thrive in a wide range of initial medium pH ranging from 3 to 11 and produced valuable lipids such as C16:0, C18:2, and C18:3 under indoor and outdoor culture conditions when supplied with 2.0% dissolved CO2. Particularly, the acidic BG11 medium effectively increased the biomass of extracellular polysaccharides during short-term outdoor cultivation. The BG11 liquid medium also led to extracellular polysaccharide production, independent of acidity and alkalinity, proportional to the increase in total sugars derived from cells supplied with high CO2 concentrations. These results suggest Parachlorella as a promising strain for indoor and outdoor cultivation to produce valuable materials.https://www.frontiersin.org/articles/10.3389/fbioe.2023.1233944/fullalgaebiorefinerycarbohydratelipidParachlorellapolysaccharide |
| spellingShingle | Akari Takagi Misato Nagao Yuya Uejima Daisaku Sasaki Munehiko Asayama Munehiko Asayama Efficient pH and dissolved CO2 conditions for indoor and outdoor cultures of green alga Parachlorella Frontiers in Bioengineering and Biotechnology algae biorefinery carbohydrate lipid Parachlorella polysaccharide |
| title | Efficient pH and dissolved CO2 conditions for indoor and outdoor cultures of green alga Parachlorella |
| title_full | Efficient pH and dissolved CO2 conditions for indoor and outdoor cultures of green alga Parachlorella |
| title_fullStr | Efficient pH and dissolved CO2 conditions for indoor and outdoor cultures of green alga Parachlorella |
| title_full_unstemmed | Efficient pH and dissolved CO2 conditions for indoor and outdoor cultures of green alga Parachlorella |
| title_short | Efficient pH and dissolved CO2 conditions for indoor and outdoor cultures of green alga Parachlorella |
| title_sort | efficient ph and dissolved co2 conditions for indoor and outdoor cultures of green alga parachlorella |
| topic | algae biorefinery carbohydrate lipid Parachlorella polysaccharide |
| url | https://www.frontiersin.org/articles/10.3389/fbioe.2023.1233944/full |
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