NcRNA-mediated upregulation of CAMK2N1 is associated with poor prognosis and tumor immune infiltration of gastric cancer
Gastric cancer (GC) is still notorious for its poor prognosis and aggressive characteristics. Though great developments have been made in diagnosis and therapy for GC, the prognosis of patient is still perishing. In this study, differentially expressed genes (DEGs) in GC were first screened using th...
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Frontiers Media S.A.
2022-08-01
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| Series: | Frontiers in Genetics |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fgene.2022.888672/full |
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| author | Kaipeng Peng Kaipeng Peng Kaipeng Peng Xiangqing Ren Xiangqing Ren Xiangqing Ren Qian Ren Qian Ren Qian Ren |
| author_facet | Kaipeng Peng Kaipeng Peng Kaipeng Peng Xiangqing Ren Xiangqing Ren Xiangqing Ren Qian Ren Qian Ren Qian Ren |
| author_sort | Kaipeng Peng |
| collection | DOAJ |
| description | Gastric cancer (GC) is still notorious for its poor prognosis and aggressive characteristics. Though great developments have been made in diagnosis and therapy for GC, the prognosis of patient is still perishing. In this study, differentially expressed genes (DEGs) in GC were first screened using three Gene Expression Omnibus (GEO) datasets (GSE13911, GSE29998, and GSE26899). Second, The Cancer Genome Atlas (TCGA) and Genotype-Tissue Expression (GTEx) data were used to validate expression of these DEGs and perform survival analysis. We selected seven candidate genes (CAMK2N1, OLFML2B, AKR7A3, CYP4X1, FMO5, MT1H, and MT1X) to carry out the next analysis. To construct the ceRNA network, we screened the most potential upstream ncRNAs of the candidate genes. A series of bioinformatics analyses, including expression analysis, correlation analysis, and survival analysis, revealed that the SNHG10–hsa-miR-378a-3p might be the most potential regulatory axis in GC. Then, the expression of CAMK2N1, miR-378a-3p, and SNHG10 was verified in GC cell lines (GES-1, MGC-803, BGC-823, HGC-27, MKN-45, and AGS) by qRT-PCR and Western blotting. We found that SNHG10 and CAMK2N1 were highly expressed in gastric cancer lines, and the miR-378a-3p was lowly expressed in BGC-823, HGC-27, and MKN-45. Furthermore, CAMK2N1 levels were significantly negatively associated with tumor immune cell infiltration, biomarkers of immune cells, and immune checkpoint expression. In summary, our results suggest that the ncRNA-mediated high expression of CAMK2N1 is associated with poor prognosis and tumor immune infiltration of GC. |
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| id | doaj.art-52b990dec7804dbeb148030a110047df |
| institution | Directory Open Access Journal |
| issn | 1664-8021 |
| language | English |
| last_indexed | 2024-04-13T03:09:40Z |
| publishDate | 2022-08-01 |
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| series | Frontiers in Genetics |
| spelling | doaj.art-52b990dec7804dbeb148030a110047df2022-12-22T03:05:06ZengFrontiers Media S.A.Frontiers in Genetics1664-80212022-08-011310.3389/fgene.2022.888672888672NcRNA-mediated upregulation of CAMK2N1 is associated with poor prognosis and tumor immune infiltration of gastric cancerKaipeng Peng0Kaipeng Peng1Kaipeng Peng2Xiangqing Ren3Xiangqing Ren4Xiangqing Ren5Qian Ren6Qian Ren7Qian Ren8The First Clinical Medical College, Lanzhou University, Lanzhou, ChinaDepartment of Gastroenterology, The First Hospital of Lanzhou University, Lanzhou, ChinaKey Laboratory for Gastrointestinal Diseases of Gansu Province, The First Hospital of Lanzhou University, Lanzhou, ChinaThe First Clinical Medical College, Lanzhou University, Lanzhou, ChinaDepartment of Gastroenterology, The First Hospital of Lanzhou University, Lanzhou, ChinaKey Laboratory for Gastrointestinal Diseases of Gansu Province, The First Hospital of Lanzhou University, Lanzhou, ChinaThe First Clinical Medical College, Lanzhou University, Lanzhou, ChinaDepartment of Gastroenterology, The First Hospital of Lanzhou University, Lanzhou, ChinaKey Laboratory for Gastrointestinal Diseases of Gansu Province, The First Hospital of Lanzhou University, Lanzhou, ChinaGastric cancer (GC) is still notorious for its poor prognosis and aggressive characteristics. Though great developments have been made in diagnosis and therapy for GC, the prognosis of patient is still perishing. In this study, differentially expressed genes (DEGs) in GC were first screened using three Gene Expression Omnibus (GEO) datasets (GSE13911, GSE29998, and GSE26899). Second, The Cancer Genome Atlas (TCGA) and Genotype-Tissue Expression (GTEx) data were used to validate expression of these DEGs and perform survival analysis. We selected seven candidate genes (CAMK2N1, OLFML2B, AKR7A3, CYP4X1, FMO5, MT1H, and MT1X) to carry out the next analysis. To construct the ceRNA network, we screened the most potential upstream ncRNAs of the candidate genes. A series of bioinformatics analyses, including expression analysis, correlation analysis, and survival analysis, revealed that the SNHG10–hsa-miR-378a-3p might be the most potential regulatory axis in GC. Then, the expression of CAMK2N1, miR-378a-3p, and SNHG10 was verified in GC cell lines (GES-1, MGC-803, BGC-823, HGC-27, MKN-45, and AGS) by qRT-PCR and Western blotting. We found that SNHG10 and CAMK2N1 were highly expressed in gastric cancer lines, and the miR-378a-3p was lowly expressed in BGC-823, HGC-27, and MKN-45. Furthermore, CAMK2N1 levels were significantly negatively associated with tumor immune cell infiltration, biomarkers of immune cells, and immune checkpoint expression. In summary, our results suggest that the ncRNA-mediated high expression of CAMK2N1 is associated with poor prognosis and tumor immune infiltration of GC.https://www.frontiersin.org/articles/10.3389/fgene.2022.888672/fullCAMK2N1gastric cancertumor immune infiltrationprognosisnoncoding RNA |
| spellingShingle | Kaipeng Peng Kaipeng Peng Kaipeng Peng Xiangqing Ren Xiangqing Ren Xiangqing Ren Qian Ren Qian Ren Qian Ren NcRNA-mediated upregulation of CAMK2N1 is associated with poor prognosis and tumor immune infiltration of gastric cancer Frontiers in Genetics CAMK2N1 gastric cancer tumor immune infiltration prognosis noncoding RNA |
| title | NcRNA-mediated upregulation of CAMK2N1 is associated with poor prognosis and tumor immune infiltration of gastric cancer |
| title_full | NcRNA-mediated upregulation of CAMK2N1 is associated with poor prognosis and tumor immune infiltration of gastric cancer |
| title_fullStr | NcRNA-mediated upregulation of CAMK2N1 is associated with poor prognosis and tumor immune infiltration of gastric cancer |
| title_full_unstemmed | NcRNA-mediated upregulation of CAMK2N1 is associated with poor prognosis and tumor immune infiltration of gastric cancer |
| title_short | NcRNA-mediated upregulation of CAMK2N1 is associated with poor prognosis and tumor immune infiltration of gastric cancer |
| title_sort | ncrna mediated upregulation of camk2n1 is associated with poor prognosis and tumor immune infiltration of gastric cancer |
| topic | CAMK2N1 gastric cancer tumor immune infiltration prognosis noncoding RNA |
| url | https://www.frontiersin.org/articles/10.3389/fgene.2022.888672/full |
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