In Vitro Studies on a Microfluidic Sensor with Embedded Obstacles Using New Antibacterial Synthetic Compounds (1-TDPPO) Mixed Prop-2-en-1-one with Difluoro Phenyl
This paper describes the use of an analytical microfluidic sensor for accelerating chemo-repellent response and strong anti-bacterial 1-(Thien-2-yl)-3-(2, 6-difluoro phenyl) prop-2-en-1-one (1-TDPPO). The chemically-synthesized antimicrobial agent, which included prop-2-en-1-one and difluoro phenyl...
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| Format: | Article |
| Language: | English |
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MDPI AG
2017-04-01
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| Series: | Sensors |
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| Online Access: | http://www.mdpi.com/1424-8220/17/4/803 |
| _version_ | 1798039443609747456 |
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| author | Changhyun Roh Jaewoong Lee Mayank Kinger Chankyu Kang |
| author_facet | Changhyun Roh Jaewoong Lee Mayank Kinger Chankyu Kang |
| author_sort | Changhyun Roh |
| collection | DOAJ |
| description | This paper describes the use of an analytical microfluidic sensor for accelerating chemo-repellent response and strong anti-bacterial 1-(Thien-2-yl)-3-(2, 6-difluoro phenyl) prop-2-en-1-one (1-TDPPO). The chemically-synthesized antimicrobial agent, which included prop-2-en-1-one and difluoro phenyl groups, was moving through an optically transparent polydimethylsiloxane (PDMS) microfluidic sensor with circular obstacles arranged evenly. The response, growth and distribution of fluorescent labeling Pseudomonas aeruginosa PAO1 against the antimicrobial agent were monitored by confocal laser scanning microscope (CLSM). The microfluidic sensor along with 1-TDPPOin this study exhibits the following advantages: (i) Real-time chemo-repellent responses of cell dynamics; (ii) Rapid eradication of biofilm by embedded obstacles and powerful antibacterial agents, which significantly reduce the response time compared to classical methods; (iii) Minimal consumption of cells and antimicrobial agents; and (iv) Simplifying the process of the normalization of the fluorescence intensity and monitoring of biofilm by captured images and datasets. |
| first_indexed | 2024-04-11T21:53:57Z |
| format | Article |
| id | doaj.art-52be39f655254c79a63139449b62beda |
| institution | Directory Open Access Journal |
| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-04-11T21:53:57Z |
| publishDate | 2017-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Sensors |
| spelling | doaj.art-52be39f655254c79a63139449b62beda2022-12-22T04:01:10ZengMDPI AGSensors1424-82202017-04-0117480310.3390/s17040803s17040803In Vitro Studies on a Microfluidic Sensor with Embedded Obstacles Using New Antibacterial Synthetic Compounds (1-TDPPO) Mixed Prop-2-en-1-one with Difluoro PhenylChanghyun Roh0Jaewoong Lee1Mayank Kinger2Chankyu Kang3Biotechnology Research Division, Advanced Radiation Technology Institute (ARTI), Korea Atomic Energy Research Institute (KAERI), 1266, Sinjeong-Dong, Jeongeup, Jeonbuk 580-185, KoreaDepartment of Textile Engineering and Technology, Yeungnam University, 280 Daehak-ro, Gyeongsan, Gyeongbuk 38541, KoreaDepartment of Chemistry, Maharishi Markandeshwar University, Mullana, (Ambala) Haryana 133207, IndiaMinistry of Employment and Labor, Major Industrial Accident Prevention Center, 34 Yeosusandallo, Yeosu-Si, Jeonnam 59631, KoreaThis paper describes the use of an analytical microfluidic sensor for accelerating chemo-repellent response and strong anti-bacterial 1-(Thien-2-yl)-3-(2, 6-difluoro phenyl) prop-2-en-1-one (1-TDPPO). The chemically-synthesized antimicrobial agent, which included prop-2-en-1-one and difluoro phenyl groups, was moving through an optically transparent polydimethylsiloxane (PDMS) microfluidic sensor with circular obstacles arranged evenly. The response, growth and distribution of fluorescent labeling Pseudomonas aeruginosa PAO1 against the antimicrobial agent were monitored by confocal laser scanning microscope (CLSM). The microfluidic sensor along with 1-TDPPOin this study exhibits the following advantages: (i) Real-time chemo-repellent responses of cell dynamics; (ii) Rapid eradication of biofilm by embedded obstacles and powerful antibacterial agents, which significantly reduce the response time compared to classical methods; (iii) Minimal consumption of cells and antimicrobial agents; and (iv) Simplifying the process of the normalization of the fluorescence intensity and monitoring of biofilm by captured images and datasets.http://www.mdpi.com/1424-8220/17/4/803PDMS microfluidic sensorconfocal laser scanning microscope1-TDPPOPseudomonas aeruginosa PAO1fluorescence intensity |
| spellingShingle | Changhyun Roh Jaewoong Lee Mayank Kinger Chankyu Kang In Vitro Studies on a Microfluidic Sensor with Embedded Obstacles Using New Antibacterial Synthetic Compounds (1-TDPPO) Mixed Prop-2-en-1-one with Difluoro Phenyl Sensors PDMS microfluidic sensor confocal laser scanning microscope 1-TDPPO Pseudomonas aeruginosa PAO1 fluorescence intensity |
| title | In Vitro Studies on a Microfluidic Sensor with Embedded Obstacles Using New Antibacterial Synthetic Compounds (1-TDPPO) Mixed Prop-2-en-1-one with Difluoro Phenyl |
| title_full | In Vitro Studies on a Microfluidic Sensor with Embedded Obstacles Using New Antibacterial Synthetic Compounds (1-TDPPO) Mixed Prop-2-en-1-one with Difluoro Phenyl |
| title_fullStr | In Vitro Studies on a Microfluidic Sensor with Embedded Obstacles Using New Antibacterial Synthetic Compounds (1-TDPPO) Mixed Prop-2-en-1-one with Difluoro Phenyl |
| title_full_unstemmed | In Vitro Studies on a Microfluidic Sensor with Embedded Obstacles Using New Antibacterial Synthetic Compounds (1-TDPPO) Mixed Prop-2-en-1-one with Difluoro Phenyl |
| title_short | In Vitro Studies on a Microfluidic Sensor with Embedded Obstacles Using New Antibacterial Synthetic Compounds (1-TDPPO) Mixed Prop-2-en-1-one with Difluoro Phenyl |
| title_sort | in vitro studies on a microfluidic sensor with embedded obstacles using new antibacterial synthetic compounds 1 tdppo mixed prop 2 en 1 one with difluoro phenyl |
| topic | PDMS microfluidic sensor confocal laser scanning microscope 1-TDPPO Pseudomonas aeruginosa PAO1 fluorescence intensity |
| url | http://www.mdpi.com/1424-8220/17/4/803 |
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