Differences in molecular characteristics and expression of virulence genes in carbapenem-resistant and sensitive Klebsiella pneumoniae isolates in Ningbo, China
BackgroundIn recent years, Klebsiella pneumoniae has attracted attention because of its increasing drug resistance. At the same time, the migration and pathogenicity caused by its virulence genes also bring many difficulties to the diagnosis and treatment of clinical infections. However, it is curre...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2024-02-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2024.1356229/full |
| _version_ | 1827356004221714432 |
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| author | Min Jiang Xuedan Qiu Siyi Shui Rongqing Zhao Wenjun Lu Chenyao Lin Yanye Tu Yifeng Wu Qingcao Li Qiaoping Wu |
| author_facet | Min Jiang Xuedan Qiu Siyi Shui Rongqing Zhao Wenjun Lu Chenyao Lin Yanye Tu Yifeng Wu Qingcao Li Qiaoping Wu |
| author_sort | Min Jiang |
| collection | DOAJ |
| description | BackgroundIn recent years, Klebsiella pneumoniae has attracted attention because of its increasing drug resistance. At the same time, the migration and pathogenicity caused by its virulence genes also bring many difficulties to the diagnosis and treatment of clinical infections. However, it is currently unclear whether there are differences in virulence and pathogenicity with changes in drug resistance.ObjectiveTo understand the differences in molecular characteristics and expression of virulence genes in carbapenem-resistant Klebsiella pneumoniae (CRKP) and carbapenem-sensitive Klebsiella pneumoniae (CSKP).MethodsUsing polymerase chain reaction (PCR), we examined capsule polysaccharide-related genes and virulence genes in 150 clinical isolates of CRKP and 213 isolates of CSKP from the local area in Ningbo, China. Multilocus sequence typing (MLST) was used to analyze the phylogenetic relationships of clinical Klebsiella pneumoniae isolates. Furthermore, real-time quantitative PCR (RT-qPCR) was used to analyze the expression differences of common virulence genes in CSKP and CRKP, and the virulence was further verified by the larval model of Galleria mellonella.ResultsThe study found that the detection rates of genes rmpA, iroB, peg-344, magA, aerobactin, alls, kfu, and entB were significantly higher in CSKP compared to CRKP. The capsule gene types K1 and K2 were more common in CSKP, while K5 was more common in CRKP. Hypervirulent Klebsiella pneumoniae (hvKP) was predominantly from CSKP. CRKP strains exhibited noticeable homogeneity, with ST11 being the predominant sequence type among the strains. CSKP strains showed greater diversity in ST types, but ST23 was still the predominant sequence type. Carbapenem-sensitive hypervirulent Klebsiella pneumoniae (CS-hvKP) had higher expression of rmpA and rmpA2 genes compared to carbapenem-resistant hypervirulent Klebsiella pneumoniae (CR-hvKP). In the wax moth virulence model, the survival rate of CS-hvKP was significantly lower than that of CR-hvKP.ConclusionThere is a significant difference in the distribution of virulence genes between CSKP and CRKP, with CSKP carrying a significantly greater number of virulence genes. Furthermore, compared to CSKP, CRKP strains exhibit noticeable homogeneity, with ST11 being the predominant sequence type among the strains. Additionally, in terms of virulence gene expression efficiency and virulence, CSKP is significantly higher than CRKP. |
| first_indexed | 2024-03-08T04:51:32Z |
| format | Article |
| id | doaj.art-52be96de4698480da07d740dcf1ada39 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-03-08T04:51:32Z |
| publishDate | 2024-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-52be96de4698480da07d740dcf1ada392024-02-08T02:45:45ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2024-02-011510.3389/fmicb.2024.13562291356229Differences in molecular characteristics and expression of virulence genes in carbapenem-resistant and sensitive Klebsiella pneumoniae isolates in Ningbo, ChinaMin Jiang0Xuedan Qiu1Siyi Shui2Rongqing Zhao3Wenjun Lu4Chenyao Lin5Yanye Tu6Yifeng Wu7Qingcao Li8Qiaoping Wu9Department of Clinical Laboratory, The Affiliated LiHuiLi Hospital of Ningbo University, Ningbo, ChinaDepartment of Clinical Laboratory, The Affiliated LiHuiLi Hospital of Ningbo University, Ningbo, ChinaDepartment of Clinical Laboratory, The Affiliated LiHuiLi Hospital of Ningbo University, Ningbo, ChinaDepartment of Clinical Laboratory, The Affiliated LiHuiLi Hospital of Ningbo University, Ningbo, ChinaDepartment of Intensive Care Units, The Affiliated LiHuiLi Hospital of Ningbo University, Ningbo, ChinaDepartment of Clinical Laboratory, The Affiliated LiHuiLi Hospital of Ningbo University, Ningbo, ChinaDepartment of Clinical Laboratory, The Affiliated LiHuiLi Hospital of Ningbo University, Ningbo, ChinaDepartment of General Surgery, The Affiliated People’s Hospital of Ningbo University, Ningbo, ChinaDepartment of Clinical Laboratory, The Affiliated LiHuiLi Hospital of Ningbo University, Ningbo, ChinaDepartment of Clinical Laboratory, The Affiliated LiHuiLi Hospital of Ningbo University, Ningbo, ChinaBackgroundIn recent years, Klebsiella pneumoniae has attracted attention because of its increasing drug resistance. At the same time, the migration and pathogenicity caused by its virulence genes also bring many difficulties to the diagnosis and treatment of clinical infections. However, it is currently unclear whether there are differences in virulence and pathogenicity with changes in drug resistance.ObjectiveTo understand the differences in molecular characteristics and expression of virulence genes in carbapenem-resistant Klebsiella pneumoniae (CRKP) and carbapenem-sensitive Klebsiella pneumoniae (CSKP).MethodsUsing polymerase chain reaction (PCR), we examined capsule polysaccharide-related genes and virulence genes in 150 clinical isolates of CRKP and 213 isolates of CSKP from the local area in Ningbo, China. Multilocus sequence typing (MLST) was used to analyze the phylogenetic relationships of clinical Klebsiella pneumoniae isolates. Furthermore, real-time quantitative PCR (RT-qPCR) was used to analyze the expression differences of common virulence genes in CSKP and CRKP, and the virulence was further verified by the larval model of Galleria mellonella.ResultsThe study found that the detection rates of genes rmpA, iroB, peg-344, magA, aerobactin, alls, kfu, and entB were significantly higher in CSKP compared to CRKP. The capsule gene types K1 and K2 were more common in CSKP, while K5 was more common in CRKP. Hypervirulent Klebsiella pneumoniae (hvKP) was predominantly from CSKP. CRKP strains exhibited noticeable homogeneity, with ST11 being the predominant sequence type among the strains. CSKP strains showed greater diversity in ST types, but ST23 was still the predominant sequence type. Carbapenem-sensitive hypervirulent Klebsiella pneumoniae (CS-hvKP) had higher expression of rmpA and rmpA2 genes compared to carbapenem-resistant hypervirulent Klebsiella pneumoniae (CR-hvKP). In the wax moth virulence model, the survival rate of CS-hvKP was significantly lower than that of CR-hvKP.ConclusionThere is a significant difference in the distribution of virulence genes between CSKP and CRKP, with CSKP carrying a significantly greater number of virulence genes. Furthermore, compared to CSKP, CRKP strains exhibit noticeable homogeneity, with ST11 being the predominant sequence type among the strains. Additionally, in terms of virulence gene expression efficiency and virulence, CSKP is significantly higher than CRKP.https://www.frontiersin.org/articles/10.3389/fmicb.2024.1356229/fullKlebsiella pneumoniaecarbapenem-resistanthvKpvirulenceMLST |
| spellingShingle | Min Jiang Xuedan Qiu Siyi Shui Rongqing Zhao Wenjun Lu Chenyao Lin Yanye Tu Yifeng Wu Qingcao Li Qiaoping Wu Differences in molecular characteristics and expression of virulence genes in carbapenem-resistant and sensitive Klebsiella pneumoniae isolates in Ningbo, China Frontiers in Microbiology Klebsiella pneumoniae carbapenem-resistant hvKp virulence MLST |
| title | Differences in molecular characteristics and expression of virulence genes in carbapenem-resistant and sensitive Klebsiella pneumoniae isolates in Ningbo, China |
| title_full | Differences in molecular characteristics and expression of virulence genes in carbapenem-resistant and sensitive Klebsiella pneumoniae isolates in Ningbo, China |
| title_fullStr | Differences in molecular characteristics and expression of virulence genes in carbapenem-resistant and sensitive Klebsiella pneumoniae isolates in Ningbo, China |
| title_full_unstemmed | Differences in molecular characteristics and expression of virulence genes in carbapenem-resistant and sensitive Klebsiella pneumoniae isolates in Ningbo, China |
| title_short | Differences in molecular characteristics and expression of virulence genes in carbapenem-resistant and sensitive Klebsiella pneumoniae isolates in Ningbo, China |
| title_sort | differences in molecular characteristics and expression of virulence genes in carbapenem resistant and sensitive klebsiella pneumoniae isolates in ningbo china |
| topic | Klebsiella pneumoniae carbapenem-resistant hvKp virulence MLST |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2024.1356229/full |
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